High-throughput RNAi screening in cultured cells: a user's guide.


Christophe J Echeverri and Norbert Perrimon. 2006. “High-throughput RNAi screening in cultured cells: a user's guide.” Nat Rev Genet, 7, 5, Pp. 373-84.


RNA interference has re-energized the field of functional genomics by enabling genome-scale loss-of-function screens in cultured cells. Looking back on the lessons that have been learned from the first wave of technology developments and applications in this exciting field, we provide both a user's guide for newcomers to the field and a detailed examination of some more complex issues, particularly concerning optimization and quality control, for more advanced users. From a discussion of cell lines, screening paradigms, reagent types and read-out methodologies, we explore in particular the complexities of designing optimal controls and normalization strategies for these challenging but extremely powerful studies.

Last updated on 08/31/2016