Cell RNAi libraries

We have an extensive collection of DNA templates for production of double stranded RNA (dsRNA) via in vitro transcription, as well as many assay-ready libraries for RNAi screening of Drosophila cells using dsRNAs.

Cell RNAi reagents and libraries are now considered a legacy service that we are ramping down as CRISPR screens have emerged as an excellent alternative, from scientific, feasibility, and cost perspectives. Please contact DRSC Director S. Mohr for more information.

  • DRSC 2.0 genome-wide screening library
  • Focused sub-libraries, including kinases and phosphatases; transcription factors; RNA binding proteins; transmembrane domain-containing proteins; ubiquitin-related genes; and more
  • approximately 3-fold coverage of fly genes with templates for dsRNA synthesis, available by request (small or large 'cherry-pick' requests) and used for building additional custom or ready-made dsRNA libraries (one or more 96-well plate of a custom selection of dsRNAs)

Please visit a specific page for more information or place a request for a cherry-picks (1, a few, or many) and custom dsRNA synthesis (96-well format) (these are ACTIVE SERVICES as of early 2024--use PPMS to request or contact the director), or, a focused library or genome-wide library (LEGACY SERVICES as of early 2024, inquire with the director, not at PPMS) online at our DRSC-FGR PPMS online ordering and instrument signup system.

liquid handling robotics is used for cherry pick and library production