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DRSC/TRiP Functional Genomics Resources & DRSC-BTRR
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DRSC/TRiP Functional Genomics Resources & DRSC-BTRR
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  • Reagents
    • in vivo RNAi fly stocks and vectors
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Protocols

Protocols

  • in vivo RNAi
    • Knockdown vectors
    • Cloning and sequencing
    • Using TRiP lines
    • GAL4-UAS in the germline
    • C911 controls
  • in vivo CRISPR
    • sgRNA vectors
    • sgRNA cloning and sequencing
    • Using TRiP-CRISPR lines
  • UAS overexpression
  • Cell culture (fly)
    • Primary cell isolation and screening
    • Single-cell isolation
    • Stable cell lines
  • Cell-based assays
    • Cell assay, fix and stain (DAPI, Phalloidin)
    • Cell assay, total ATP
  • Cell-based RNAi
    • dsRNA synthesis
    • Cell RNAi (6-well)
    • Cell RNAi (384-well)
  • Cell-based CRISPR
  • Drosophila Protocols Portal
  • Shipping reagent library plates

Protocols for in vivo studies include

  • RNAi
  • CRISPR approaches
  • overexpression

Protocols for cell-based approaches include

  • single-cell isolation
  • making stable cell lines
  • isolation of primary cells from embryos
  • fix and stain for cell imaging
  • treatment of cells with RNAi (6-well format; 384-well format)

Looking in general for Drosophila protocols?

Check out our beta test of a Drosophila protocols portal.

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DRSC/TRiP Functional Genomics Resources & DRSC-BTRR
Harvard Medical School
Dept. of Genetics | Blavatnik Institute
New Research Building Room 336

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77 Avenue Louis Pasteur
Boston, MA 02115
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Fax: (617) 432-7688

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