Features of all vectors used for in vivo sgRNA expression in TRIP-CRISPR stocks:

• vermilion as a selectable marker
• attB sequence to allow for phiC31 targeted integration at genomic attP landing sites
• ubiquitous sgRNA expression driven by one or more RNA polymerase III-dependent promoters from U6 snRNA genes

flySAM2.0 (Jia et al., 2018)

• ubiquitously expresses one sgRNA containing MS2 loops from the U6:2 promoter
• UAS control of dCas9-VP64-T2A-MCP
• vector for the TRiP-CRISPR Overexpression (TriP-OE) flySAM2.0 Collection
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U6B-sgRNA2.0 (Jia et al., 2018)

• ubiquitously expresses one sgRNA containing MS2 loops from the U6:2 promoter
• vector for the TRiP-CRISPR Overexpression (TriP-OE) flySAM1.0 Collection
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pCFD3 (Port, Chen, Lee, & Bullock, 2014)

• ubiquitously expresses one sgRNA from the U6:3 promoter
• vector for TRiP-CRISPR Knockout (TriP-KO) Collection
• For more information visit http://www.crisprflydesign.org/plasmids/ and addgene
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pCFD4 (Port, Chen, Lee, & Bullock, 2014)

• ubiquitously expresses two sgRNAs from the U6:1 and U6:3 promoters
• primary vector of the TRiP-CRISPR Overexpression (TRiP-OE) VPR Collection
• For more information visit http://www.crisprflydesign.org/plasmids/ and addgene
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pCFD4-MS2

• ubiquitously expresses two sgRNAs containing MS2 loops from the U6:1 and U6:3 promoters
• compatible with the Synergistic Activation Mediator (SAM) system (Konermann et al., 2015)
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pl100 (Kondo and Ueda, 2013)

• ubiquitously expresses one sgRNA from the U6:1 promoter
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