ORF plasmid collections

Libraries such as UAS-cDNA collections can be incorporated into cell-based screening assays, so that you can test the effect of ectopic expression either alongside or instead of taking an RNAi-based knockdown approach.

UAS-ORF Collection. A set of 2,600 UAS-ORFs for over-expression screening in cells at the DRSC (collaboration with S. Celniker at BDGP and D. Root at the Broad RNAi Platform). The ORFs are searchable withGeneLookup. Please contact us for more information.

UAS-miRNA Collection. Purified DNA or bacterial glycerol stocks for approximately 150 UAS-miRNAs. On-site screening or off-site use. Reference: Silver SJ, Hagen JW, Okamura K, Perrimon N, Lai EC. Functional screening identifies miR-315 as a potent activator of Wingless signaling. Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18151-6. PubMed ID: 17989227 

See also, TRiP VALIUM series vectors for overexpression.