Quick link to Find CRISPR sgRNA search
Quick link to TRiP-CRISPR sgRNA stock database
Using the TRiP pipeline, we are actively developing CRISPR fly stocks and resources for gene editing and other applications. These resources facilitate knockout, up-regulation (CRIPSRa), and other Drosophila in vivo CRISPR applications.
TRiP-CRISPR fly stock collections |
Function |
sgRNA Vectors |
Cross to |
Notes |
|
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Gene Activation |
Gal4+dCas9-VPR |
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Gene Activation |
Gal4+flySAM |
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Gene Activation |
Gal4 |
flySAM2.0 lines contain both sgRNA and dCas9 (flySAM) |
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Gene Cutting |
Gal4+Cas9 |
Search the DRSC/TRiP sgRNA Stock Database for TRiP-OE and TRiP-KO stocks
Nominate genes for production for TRiP-OE and TRiP-KO stocks
Download a .XLS file of currently available TRiP-OE and TRiP-KO stocks
Protocols for cloning, sequencing, and using TRiP-CRISPR lines
Online tools for gRNA design and efficiency prediction
See links below to relevant online tools, reagents, protocols, publications, and more.