TRiP-CRISPR Overexpression (TRiP-OE)

For activation of target gene expression

 

TRiP-OE: The flySAM2.0 Collection

  • New simplified strategy for in vivo CRISPR activation
  • flySAM generates stronger overexpression phenotypes, comparable to Gal4/UAS
  • Activate expression of target gene by crossing to any Gal4 line

flySAM

 

 

TRiP-OE: The VPR Collection

  • Activate expression of target gene by crossing to Gal4>dCas9-VPR flies
  • Generate InDels and larger genomic deletions by crossing to Gal4>Cas9 flies
  • For a detailed description of the the TRiP-OE gene activation approach click here

VPR

 

About TRiP-OE stocks

flySAM

  • A single gene is targeted by expression of one sgRNA from the U6:2 promoter
  • Stocks are made in the flySAM2.0 vector, developed by Jian-quan Ni and colleagues
  • TRiP-OE/flySAM stocks contain UAS-Cas9, so simply crossing to a Gal4 induces expression of the target gene
  • To learn more about the TRiP-OE vectors, sgRNA design, and  gene activation please visit the in vivo CRISPR protocols page

VPR

  • A single gene is targeted by tandem expression of two sgRNAs from independent U6 promoters
  • Stocks are made in the pCFD4 vector, developed by Fillip Port and colleagues (http://www.crisprflydesign.org/plasmids)
  • Crossing TRiP-OE stocks to a Gal4 line expressing dCas9 fused to the chimeric activator domain VPR induces expression of the target gene
  • To learn more about the TRiP-OE vectors, sgRNA design, and  gene activation please visit the in vivo CRISPR protocols page

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