TRiP RNAi control fly stocks

The following TRiP stocks can be used as experimental controls. These lines are available to order from the The following TRiP stocks can be used as experimental controls. These lines are available to order from the Bloomington Drosophila Stock Center. 

Background Stocks
Site
Vector
Name
Description
BDSC#
attP2
 
y v; attP2, y+
y[1] v[1] ; P{y[+t7.7]=CaryP}attP2
36303
attP40
 
y v; attP40, y+
y[1] v[1] ; P{y[+t7.7]=CaryP}attP40
36304
Overexpression Stocks
Site
Vector
Name
Description
BDSC#
attP2
VALIUM10 
GFP (from Wild Type)
Overexpression of GFP in VALIUM10 attP2.Can be used as a control for VALIUM10 or VALIUM20.
35786
VALIUM20
attP2
VALIUM10 
mCherry
Overexpression of mCherry in VALIUM10 attP2. Can be used as a control for VALIUM10 or VALIUM20.
35787
VALIUM20
attP2
VALIUM10 
Luciferase
Overexpresses luciferase in VALIUM10 attP2. Can be used as a control for VALIUM10 or VALIUM20.
35788
VALIUM20
attP2
VALIUM1
Luciferase
Overexpresses luciferase in a VALIUM1 attP2 background.
35789
Hairpin Stocks
Site
Vector
Name
Description
BDSC#
attP2
VALIUM20
Gal4-1
Hairpin targeting Gal4 in VALIUM20.
35784
 
Top: 
ctagcagtCAGGATTATTTGTACAAGATAtagttatattcaagcata TATCTTGTACAAATAATCCTGgcg 
Bottom: aattcgcCAGGATTATTTGTACAAGATAtatgcttgaatataacta TATCTTGTACAAATAATCCTGactg
attP2
VALIUM20
Gal4-2
Hairpin targeting Gal4 in VALIUM20.
35783
 
Top: ctagcagtTCGGAAGAGAGTAGTAACAAAtagttatattcaagcata TTTGTTACTACTCTCTTCCGAgcg 
Bottom: aattcgcTCGGAAGAGAGTAGTAACAAAtatgcttgaatataacta TTTGTTACTACTCTCTTCCGAactg
attP2
VALIUM20
mCherry
Hairpin targeting mCherry in VALIUM20.
35785
 
Top: ctagcagtCGAGTTCATCTACAAGGTGAAtagttatattcaagcata TTCACCTTGTAGATGAACTCGgcg 
Bottom: aattcgcCGAGTTCATCTACAAGGTGAAtatgcttgaatataacta TTCACCTTGTAGATGAACTCGactg
attP2
VALIUM20
YFP
Hairpin targeting YFP in VALIUM20.
TBD
 
Top: ctagcagtCAAGCTGGAGTACAACTACAAtagttatattcaagcata TTGTAGTTGTACTCCAGCTTGgcg
Bottom: aattcgcCAAGCTGGAGTACAACTACAAtatgcttgaatataacta TTGTAGTTGTACTCCAGCTTGactg
attP2
VALIUM1
Luciferase
Hairpin against firefly luciferase from the firefly Photinus pyralis in VALIUM1.
31603