TRiP toolbox fly stocks

Through the BDSC, the TRiP provides the community with the “TRiP Toolbox”, which includes injection stocks for labs wishing to generate their own RNAi lines and commonly used GAL4 lines with UAS-Dcr2 to enhance message knockdown.

Injection Stocks Genotype BL Stock#
y sc v nanos-integrase; attP40 y[1] sc[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X; P{y[+t7.7]=CaryP}attP40  
y v nanos-integrase; attP40 y[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X; P{y[+t7.7]=CaryP}attP40 25709
y sc v nanos-integrase; attP2 y[1] sc[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X; P{y[+t7.7]=CaryP}attP2 25710
Gal4, UAS dcr2 Stocks Genotype BL Stock#
w, elav-Gal4; UAS-dcr2 w[1118], P{w[+mC]=GAL4-elav.L}; P{w[+mC]=UAS-Dcr-2.D}2 25750
w, ms1096-Gal4; UAS-dcr2 w[1118], P{w[+mW.hs]=GawB}Bx[MS1096]; P{w[+mC]=UAS-Dcr-2.D}2 25706
w, UAS-dcr2; twist-Gal4 P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mC]=GAL4-twi.2xPE}1 25707
w, UAS-dcr2; actin-Gal4/CyO P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mC]=Act5C-GAL4}25FO1 / CyO, Cy[1] 25708
w, UAS-dcr2; nanos-Gal4 P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mC]=GAL4-nos.NGT}40 25751
w, UAS-dcr2; engrailed-Gal4, UAS-GFP P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2 25752
w, UAS-dcr2; blistered-Gal4/CyO P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mC}=bs-GAL4.Term}G1 25753
w, UAS-dcr2; nubbin-Gal4 P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mW.hs]=GawB}nubbin-AC-62 25754
w, UAS-dcr2; spalt-Gal4 P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mW.hs]=GawB}salm[LP39] 25755
w, UAS-dcr2; Dmef2-Gal4 P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mC]=GAL4-Mef2.R}R1 25756
w, UAS-dcr2; C96-Gal4 P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mW.hs]=GawB}bbg[C96] 25757
w, UAS-dcr2; pannier-Gal4/TM3, Ser P{w[+mC]=UAS-Dcr-2.D}1, w[1118]; P{w[+mW.hs]=GawB}pnr[MD237] /TM3, Ser[1] 25758
Gal4 Stocks Genotype BL Stock#
MTD-Gal4 P{otu-GAL4::VP16.R}1, w[*]; P{GAL4-nos.NGT}40; P{GAL4::VP16-nos.UTR}CG6325[MVD1] (also known as MTD-GAL4) 31777
Mapping Stocks Genotype BL Stock#
y sc v; Gla Bc/CyO y[1] sc[1] v[1]; wg[Gla-1], Bc[1] / CyO, Cy[1]  
y v; Sco/CyO y[1] v[1]; noc[Sco] / CyO, Cy[1]  
y v; TM3, Sb/TM6, Tb y[1] v[1]; TM3, Sb[1] / TM6, Tb[1]  
y v; Ly/TM3, Sb y[1] v[1]; sens[Ly-1] / TM3, Sb[1]  
y v; Sb/TM3, Ser y[1] v[1]; Sb[1] / TM3, Ser[1]  
y v; Dr, e/ TM3, Sb y[1] v[1]; Dr[1] e[1] / TM3, Sb[1]  
y sc v; Dr, e/ TM3, Sb y[1] sc[1] v[1]; Dr[1] e[1] / TM3, Sb[1] 32261