TRiP-CRISPR toolbox fly stocks

Cas9 stocks for use with TRiP-CRISPR sgRNA fly stock collections

Completed TRiP-CRISPR/CAS9 Toolbox stocks are available at the BDSC.

Click here to download list in .XLS format or see below

Gal4+VPR (tissue-specific gene overexpression)

TRiP-OE VPR cross

Cross Gal4+dCas9-VPR stocks to sgRNA stocks from the TRiP-OE VPR collection (sgRNAs cloned into pCFD4) to overexpress target gene.

GAL4/UAS expression of Cas9 proteins with dead nuclease activity (dCas9), fused to VPR transcriptional activator (dCas9-VPR).

Not compatible with TRiP-OE sgRNA stocks from the flySAM collections (sgRNAs cloned into U6BsgRNA2.0 or flySAM2.0).

Gene activation - cross to TRiP-OE sgRNA stocks (VPR colletion)

Nickname
Availability
Genotype
elav>VPR
in progress
P{w[+mW.hs]=GawB}elav[C155];P{UAS-3XFLAG-dCas9-VPR}attp40/CyO
MS1096>VPR
67039
w[1118] P{w[+mW.hs]=GawB}Bx[MS1096];P{UAS-3XFLAG-dCas9-VPR}attp40/CyO
pnr>VPR
67040
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/(TM6B,Tb[1])
dMef2 > VPR
67041
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mC]=GAL4-Mef2.R}R1/(TM6B,Tb[1])
C96 > VPR
67042
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mW.hs]=GawB}bbg[C96]/TM6B,Tb[1]
Lpp > VPR
67043
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO;P{Lpp-GAL4.B}/TM6B,Tb[1]
Mhc > VPR
67044
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO;P{[ry+]MHC-82-Gal4}/TM6B, Tb[1]
dpp > VPR
67045
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mW.hs]=GAL4-dpp.blk1}TM6B,Tb[1]
hh > VPR
67046
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; hh-Gal4 /TM6B,Tb[1]
Delta[ts] > VPR
in progress
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO;P{w[+mW.hs]=GawB}Dl[05151] ,P{w[+mC]=tubP-GAL80[ts]}[*] /TM6B,Tb[1]
tubulin > VPR
in progress
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; tub-Gal4 /TM6B,Tb[1]
tubulin > VPR
67048
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40; tub-Gal4 / SM5;TM6B Tb[1]
en > VPR
67049
w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/ CyO;UASP{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1]
spalt > VPR
67050
w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1]
blistered > VPR
67051
w[*]; P{w[+mC]=bs-GAL4.Term}G1/ CyO;UAS:P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1]
nos > VPR
67052
w[*]; P{w[+mC]=GAL4-nos.NGT}40 / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1]
twi > VPR
67053
w[*]; P{w[+mC]=GAL4-twi.2xPE}1 / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1]
esg > VPR + GFP
67054
w[*];esg-Gal4,P{ UAS-GFP} / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1]
nubbin > VPR
67055
w[*]; P{w[+mW.hs]=GawB}nubbin-AC-62 / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1]
3.1Lsp2 > VPR
in progress
w[*]; P{w{[+mC]=Gal4-Lsp2-3.1} / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1]
Myo1A > VPR
67057
w[*]; P{GawB}Myo31DFNP0001/ CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1]

Temporal control of gene activation - cross to TRiP-OE sgRNA stocks (VPR colletion)

Nickname
Availability
Genotype
elav[ts] > VPR
67058
P{w[+mW.hs]=GawB}elav[C155]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO
Lpp[ts] > VPR
67061
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO;P{Lpp-GAL4.B}/TM6B,Tb[1]
Mhc[ts] > VPR
67062
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO; P{[ry+]MHC-82-Gal4}/TM6B, Tb[1]
dMef2[ts] > VPR
67063
w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO; P{w[+mC]=GAL4-Mef2.R}R1/(TM6B,Tb[1])
Myo1A[ts] > VPR
67067
w[*]; P{GawB}Myo31DFNP0001/CyO; P{UAS-3XFLAG-dCas9-VPR}attp2, P{w[+mC]=tubP-GAL80[ts]}2/TM6B,Tb[1]
3.1Lsp2 > VPR
in progress
w[*]; P{w{[+mC]=Gal4-Lsp2-3.1}/CyO; P{UAS-3XFLAG-dCas9-VPR}attp2, P{w[+mC]=tubP-GAL80[ts]}2/TM6B,Tb[1]
bs[ts]> VPR
67071
w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
en[ts]> VPR
67069
w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
spalt[ts] > VPR
67070
w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2
dpp[ts]> VPR
67066
w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GAL4-dpp.blk1}40C.6/TM6B, Tb[1]
C96[ts] > VPR
67064
w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mW.hs]=GawB}bbg[C96]
MS1096[ts]>VPR
67059
w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10
Dl[ts]> VPR
67047
w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mW.hs]=GawB}Dl[05151-G], P{w[+mC]=tubP-GAL80[ts]}2/TM6B, Tb[1]
pnr[ts]>VPR
67060
w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/TM6B, Tb[1]
tubulin > VPR
67065
w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[1]
esg[ts]> VPR
67072
w[*]; P{w[+mW.hs]=GawB}NP5130 P{w[+*]=UAS-GFP.U}2; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2

 

 

Gal4+Cas9 (for tissue specific gene KO)

Gal4+Cas9 stocks

Cross Gal4+Cas9 stocks to sgRNA stocks from the TRiP-KO collection (sgRNAs cloned into pCFD3 or pCFD4) to cut target gene.

Somatic tissue-specific GAL4/UAS expression of Cas9

Gene cutting/knockout in somatic cells to make mutant tissue - cross to TRiP-KO sgRNA stocks

Nickname
Availability
Genotype
elav > Cas9
67073
P{w[+mW.hs]=GawB}elav[C155]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO
ms1096 > Cas9
67074
w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO
dMef2 > Cas9
67075
w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mC]=GAL4-Mef2.R}R1/(TM6B,Tb[1])
C96 > Cas9
67076
w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mW.hs]=GawB}bbg[C96]/TM6B,Tb[1]
Pannier > Cas9
67077
w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/(TM6B,Tb[1])
Lpp > Cas9
in progress
w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{Lpp-GAL4.B}/TM6B,Tb[1]
Mhc > Cas9
67079
w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{[ry+]MHC-82-Gal4}/TM6B, Tb[1]
actin > Cas9
in progress
w[*]; P{w[+mC]=Act5C-GAL4}25FO1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu
en > Cas9
in progress
w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu
spalt > Cas9
67081
w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu
blistered > Cas9
67082
w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu
twi > Cas9
67084
w[*]; P{w[+mC]=GAL4-twi.2xPE}1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu
esg > Cas9 + GFP
67085
w[*];esg-Gal4,P{ UAS-GFP}/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B
nubbin > Cas9
67086
w[*]; P{w[+mW.hs]=GawB}nubbin-AC-62/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu
3.1Lsp2 > Cas9
67087
w[*]; P{w{[+mC]=Gal4-Lsp2-3.1}/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B
Myo1A > Cas9
67088
w[*]; P{GawB}Myo31DFNP0001/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu

 

nanos-Cas9 (for gene KO in germline)

Germline+Cas9

Cross nanos+Cas9 stocks to sgRNA stocks from the TRiP-KO collection (sgRNAs cloned into pCFD3 or pCFD4) to cut target gene.

germline-specific GAL4/UAS expression of Cas9 to make mutant animals

Gene cutting/knockout in somatic cells to make mutant tissue - cross to TRiP-KO sgRNA stocks

Nickname
Availability
Genotype
nos-Cas9
in progress
y[1] sc[*] v[1]; P{Nos-Cas9}Attp40
nos-Cas9
in progress
y[1] sc[*] v[1]; P{Nos-Cas9}Attp2
nos > Cas9
67083
w[*]; P{w[+mC]=GAL4-nos.NGT}40/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu