Publications
Sort & Filters
Filters
221 results
221 results
2024
Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
CRISPR screens enable systematic, scalable genotype-to-phenotype mapping. We previously developed a pooled CRISPR screening method for and mosquito cell lines using plasmid transfection and site-specific integration to introduce single guide (sgRNA)...
Ah-Ram Kim, Yanhui Hu, Aram Comjean, Jonathan Rodiger, Stephanie Mohr, and Norbert Perrimon. 2024. “Enhanced Protein-Protein Interaction Discovery via AlphaFold-Multimer”. BioRxiv, Pp. 2024.02.19.580970
Ah-Ram Kim, Yanhui Hu, Aram Comjean, Jonathan Rodiger, Stephanie Mohr, and Norbert Perrimon. 2024. “Enhanced Protein-Protein Interaction Discovery via AlphaFold-Multimer”. BioRxiv, Pp. 2024.02.19.580970
Accurately mapping protein-protein interactions (PPIs) is critical for elucidating cellular functions and has significant implications for health and disease. Conventional experimental approaches, while foundational, often fall short in capturing direct...
2023
Nikki Coleman-Gosser, Yanhui Hu, Shiva Raghuvanshi, Shane Stitzinger, Weihang Chen, Arthur Luhur, Daniel Mariyappa, Molly Josifov, Andrew Zelhof, Stephanie Mohr, Norbert Perrimon, and Amanda Simcox. 2023. “Continuous Muscle, Glial, Epithelial, Neuronal, and Hemocyte Cell Lines for Research”. Elife, 12. doi:10.7554/eLife.85814
Nikki Coleman-Gosser, Yanhui Hu, Shiva Raghuvanshi, Shane Stitzinger, Weihang Chen, Arthur Luhur, Daniel Mariyappa, Molly Josifov, Andrew Zelhof, Stephanie Mohr, Norbert Perrimon, and Amanda Simcox. 2023. “Continuous Muscle, Glial, Epithelial, Neuronal, and Hemocyte Cell Lines for Research”. Elife, 12. doi:10.7554/eLife.85814
Expression of activated Ras, Ras, provides cultured cells with a proliferation and survival advantage that simplifies the generation of continuous cell lines. Here, we used lineage-restricted Ras expression to generate continuous cell lines of muscle...
Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells”. Elife, 12. doi:10.7554/eLife.85542
Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells”. Elife, 12. doi:10.7554/eLife.85542
Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes...
Hong-Wen Tang, Kerstin Spirohn, Yanhui Hu, Tong Hao, István Kovács, Yue Gao, Richard Binari, Donghui Yang-Zhou, Kenneth Wan, Joel Bader, Dawit Balcha, Wenting Bian, Benjamin Booth, Atina Coté, Steffi de Rouck, Alice Desbuleux, Kah Goh, Dae-Kyum Kim, Jennifer Knapp, Wen Lee, Irma Lemmens, Cathleen Li, Mian Li, Roujia Li, Hyobin Lim, Yifang Liu, Katja Luck, Dylan Markey, Carl Pollis, Sudharshan Rangarajan, Jonathan Rodiger, Sadie Schlabach, Yun Shen, Dayag Sheykhkarimli, Bridget TeeKing, Frederick P. Roth, Jan Tavernier, Michael Calderwood, David Hill, Susan Celniker, Marc Vidal, Norbert Perrimon, and Stephanie E. Mohr. 2023. “Next-Generation Large-Scale Binary Protein Interaction Network for Drosophila Melanogaster”, 14, 1, Pp. 2162
Hong-Wen Tang, Kerstin Spirohn, Yanhui Hu, Tong Hao, István Kovács, Yue Gao, Richard Binari, Donghui Yang-Zhou, Kenneth Wan, Joel Bader, Dawit Balcha, Wenting Bian, Benjamin Booth, Atina Coté, Steffi de Rouck, Alice Desbuleux, Kah Goh, Dae-Kyum Kim, Jennifer Knapp, Wen Lee, Irma Lemmens, Cathleen Li, Mian Li, Roujia Li, Hyobin Lim, Yifang Liu, Katja Luck, Dylan Markey, Carl Pollis, Sudharshan Rangarajan, Jonathan Rodiger, Sadie Schlabach, Yun Shen, Dayag Sheykhkarimli, Bridget TeeKing, Frederick P. Roth, Jan Tavernier, Michael Calderwood, David Hill, Susan Celniker, Marc Vidal, Norbert Perrimon, and Stephanie E. Mohr. 2023. “Next-Generation Large-Scale Binary Protein Interaction Network for Drosophila Melanogaster”, 14, 1, Pp. 2162
Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein...
Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness”. BioRxiv, Pp. 2023.08.31.555785
Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness”. BioRxiv, Pp. 2023.08.31.555785
Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account for a subset of existing arthropod species in nature. Here, we...
Nisha Singh, Agustin Rolandelli, Anya O’Neal, Rainer Butler, Sourabh Samaddar, Hanna Laukaitis-Yousey, Matthew Butnaru, Stephanie Mohr, Norbert Perrimon, and Joao Pedra. 2023. “Genetic Manipulation of an Ixodes Scapularis Cell Line”. BioRxiv, Pp. 2023.09.08.556855
Nisha Singh, Agustin Rolandelli, Anya O’Neal, Rainer Butler, Sourabh Samaddar, Hanna Laukaitis-Yousey, Matthew Butnaru, Stephanie Mohr, Norbert Perrimon, and Joao Pedra. 2023. “Genetic Manipulation of an Ixodes Scapularis Cell Line”. BioRxiv, Pp. 2023.09.08.556855
Although genetic manipulation is one of the hallmarks in model organisms, its applicability to non-model species has remained difficult due to our limited understanding of their fundamental biology. For instance, manipulation of a cell line originated...
Stephanie Mohr, Ah-Ram Kim, Yanhui Hu, and Norbert Perrimon. 2023. “Finding Information about Uncharacterized Drosophila Melanogaster Genes”. Genetics
Stephanie Mohr, Ah-Ram Kim, Yanhui Hu, and Norbert Perrimon. 2023. “Finding Information about Uncharacterized Drosophila Melanogaster Genes”. Genetics
Genes that have been identified in the genome but remain uncharacterized with regards to function offer an opportunity to uncover novel biological information. Novelty is exciting but can also be a barrier. If nothing is known, how does one start...
2022
Ah-Ram Kim, Jun Xu, Ross Cheloha, Stephanie Mohr, Jonathan Zirin, Hidde Ploegh, and Norbert Perrimon. 2022. “NanoTag Nanobody Tools for Drosophila In Vitro and In Vivo Studies”. Curr Protoc, 2, 12, Pp. e628. doi:10.1002/cpz1.628
Ah-Ram Kim, Jun Xu, Ross Cheloha, Stephanie Mohr, Jonathan Zirin, Hidde Ploegh, and Norbert Perrimon. 2022. “NanoTag Nanobody Tools for Drosophila In Vitro and In Vivo Studies”. Curr Protoc, 2, 12, Pp. e628. doi:10.1002/cpz1.628
Nanobodies have emerged as powerful protein-binding tools to uncover protein functions. Using functionalized protein binders, proteins of interest can be visualized, degraded, delocalized, or post-translationally modified in vivo. We recently reported the...
Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7
Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7
Entomopathogenic nematodes are widely used as biopesticides1,2. Their insecticidal activity depends on symbiotic bacteria such as Photorhabdus luminescens, which produces toxin complex (Tc) toxins as major virulence factors3-6. No protein receptors are...
Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430
Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430
Partial loss-of-function mutations in glycosylation pathways underlie a set of rare diseases called Congenital Disorders of Glycosylation (CDGs). In particular, DPAGT1-CDG is caused by mutations in the gene encoding the first step in N-glycosylation...
Arpan Ghosh, Yanhui Hu, Sudhir Gopal Tattikota, Yifang Liu, Aram Comjean, and Norbert Perrimon. 2022. “Modeling Exercise Using Optogenetically Contractible Drosophila Larvae”. BMC Genomics, 23, 1, Pp. 623. doi:10.1186/s12864-022-08845-6
Arpan Ghosh, Yanhui Hu, Sudhir Gopal Tattikota, Yifang Liu, Aram Comjean, and Norbert Perrimon. 2022. “Modeling Exercise Using Optogenetically Contractible Drosophila Larvae”. BMC Genomics, 23, 1, Pp. 623. doi:10.1186/s12864-022-08845-6
The pathophysiological effects of a number of metabolic and age-related disorders can be prevented to some extent by exercise and increased physical activity. However, the molecular mechanisms that contribute to the beneficial effects of muscle activity...