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Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos [NOTE: A modified final version was published in Nat Comm and is now available.]

Citation:

Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 3/30/2021. “Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos [NOTE: A modified final version was published in Nat Comm and is now available.].” bioRxiv. Publisher's Version
2021.03.29.437496v2.full_.pdf2.99 MB

Abstract:

Mosquito-borne diseases present a worldwide public health burden. Genome-scale screening tools that could inform our understanding of mosquitos and their control are lacking. Here, we adapt a recombination-mediated cassette exchange system for delivery of CRISPR sgRNA libraries into cell lines from several mosquito species and perform pooled CRISPR screens in an Anopheles cell line. To implement this method, we engineered modified mosquito cell lines, validated promoters and developed bioinformatics tools for multiple mosquito species.Competing Interest StatementThe authors have declared no competing interest.
See also: DRSC-BTRR, CRISPR gRNA design, Cell-based CRISPR, CRISPR gRNA design, CRISPR genome editing, DRSC-BTRR, Bioinformatics, DRSC-BTRR, Dataset or data portal, Research report, Aedes aegypti, Culex quinquefasciatus, Aedes albopictus, Anopheles gambiae, Drosophila (fly)
Last updated on 09/06/2022