Three sgRNA libraries for CRISPR knockout screening in Drosophila cells that together cover the full Drosophila genome are available from Addgene. A Cas9 cell line useful for screening is available at the DGRC. We also published a protocol manuscript in Current Protocols in Molecular Biology describing the pooled screen process in detail (Viswanatha et al. 2019; please see below).
We are here to help! Please reach out to DRSC Director S. Mohr for more information about pooled-format screening in Drosophila cultured cells. We are developing new assays, libraries, and cell lines, and we welcome inquiries about these new technologies.