Cell CRISPR screen reagents
Three sgRNA libraries for CRISPR knockout screening in Drosophila cells that together cover the full Drosophila genome are available from Addgene. A Cas9 cell line useful for screening is available at the DGRC. We also published a protocol manuscript in Current Protocols in Molecular Biology describing the pooled screen process in detail (Viswanatha et al. 2019; please see below).
We are here to help! Please reach out to DRSC Director S. Mohr for more information about pooled-format screening in Drosophila cultured cells. We are developing new assays, libraries, and cell lines, and we welcome inquiries about these new technologies.
Publications
2024
Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595
Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595
Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
2023
Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells”. Elife, 12. doi:10.7554/eLife.85542
Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells”. Elife, 12. doi:10.7554/eLife.85542
Nisha Singh, Agustin Rolandelli, Anya O’Neal, Rainer Butler, Sourabh Samaddar, Hanna Laukaitis-Yousey, Matthew Butnaru, Stephanie Mohr, Norbert Perrimon, and Joao Pedra. 2023. “Genetic Manipulation of an Ixodes Scapularis Cell Line”. BioRxiv, Pp. 2023.09.08.556855
Nisha Singh, Agustin Rolandelli, Anya O’Neal, Rainer Butler, Sourabh Samaddar, Hanna Laukaitis-Yousey, Matthew Butnaru, Stephanie Mohr, Norbert Perrimon, and Joao Pedra. 2023. “Genetic Manipulation of an Ixodes Scapularis Cell Line”. BioRxiv, Pp. 2023.09.08.556855
Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness”. BioRxiv, Pp. 2023.08.31.555785
Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness”. BioRxiv, Pp. 2023.08.31.555785
2022
Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7
Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7
Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430
Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430
Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
2021
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3
J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “Precise Genome Engineering in Drosophila Using Prime Editing”. Proc Natl Acad Sci U S A, 118
J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “Precise Genome Engineering in Drosophila Using Prime Editing”. Proc Natl Acad Sci U S A, 118
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos [NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.]”. BioRxiv. doi:10.1101/2021.03.29.437496
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos [NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.]”. BioRxiv. doi:10.1101/2021.03.29.437496
Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. BioRxiv
Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. BioRxiv