CRISPR Efficiency Prediction

Part of our "Find CRISPRs" design suite, our Efficiency Predictor evaluation tool compares the nucleotide sequence of short guide RNA (gRNA) designs against our algorithms for optimal efficiency. Note that this tool does not evaluate potential off targets.





To request a tutorial video, please make the suggestion on our bug report form.


Please see our CRISPR online tools documentation page.

Additional Resources

You might also be interested in our Find CRISPRs tool for identifying gRNAs and our high-resolution melt analysis (HRMA) tool relevant to detection of indels. For lists of additional resources for applying CRISPR technologies in Drosophila, we recommend visiting the FlyBase CRISPR resources page, the CRISPR fly design website (F. Port), and the flyCRISPR website

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