DRSC 2.0 genome-wide screening library

The DRSC Genome-wide RNAi Library (DRSC 2.0)

The DRSC 2.0 is a collection of dsRNAs for genome-wide RNAi knockdown. It improves uponversion 1.0 in several ways, most significantly in terms of minimizing the potential for off-target effects due to sequence similarity to genes other than the target. The library is regularly updated to accommodate newly identified genes and updated gene models.

Features include:

  • About 13,900 genes are represented in the collection (66 assay plates)
  • Average of one to two dsRNAs per gene
  • Coverage includes genes that encode proteins and non-coding RNAs
  • Genome-wide coverage lets you screen in an unbiased manner
  • Adheres to current rules for minimizing off-target effects
  • Most-used library at the DRSC, with many successful publications resulting from work on hits identified using this library

The control well layout for genome assay plates are available

Availability. The DRSC 2.0 is available for on-site screening. In addition, a limited number of amplicons can be requested as PCR-amplified DNA

The DRSC Collection for Follow-Up with Distinct Reagents (DRSC Follow-Up)

DRSC Follow-Up is a collection of amplicons for RNAi knockdown of genes that have previously scored as hits in any screen at the DRSC.

As of spring 2008, DRSC Follow-Up covered about 6,000 amplicons. Amplicons directed against unrepresented genes are generated when new lists of screen hits submitted by screeners. Our ultimate goal is for genome-wide coverage.

Importantly, the DRSC Follow-Up amplicons are distinct from the amplicons in the 2.0 amplicons (i.e. target a different region of the gene), providing independent validation of a positive RNAi result. Please note that confirmation of the RNAi result does not eliminate the need for other follow-up, such as in vivo confirmation of biological relevance.

Availability. Screen hits can be requested as "cherry picks" from the DRSC Follow-Up collection. The requested amplicons are provided as PCR-amplified DNA templates. These can be used for dsRNA production at the home institution.