September 13, 2021
New this fall: Online office hours!
Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly stocks? Questions about our new protocols and resources for CRISPR mosquito cell lines? Pop into our Zoom office hours to say hello and get our expert input! Registration is required (see below).
DRSC/TRiP & DRSC-BTRR Office Hours Schedule:
Mon. Sept. 27, 2021, 12 pm EST, Drosophila cell technologies
[Missed it? View a summary info file here.]
· Drosophila cell culture and media
· Drosophila cell transfection by lipofection or electroporation
· Drosophila cell knockout, knock-in, and RNAi
· Drosophila cell CRISPR knockout screening
· Drosophila cell CRISPR activation screening
Mon. Oct. 4, 2021, 12 pm EST, Drosophila in vivo RNAi technologies
[Missed it? View a summary info file here.]
[Missed it? View a summary info file here.]
· In vivo RNAi knockdown using TRiP fly stocks
· Controls and caveats to in vivo RNAi
· RSVP Plus online resource for lookup of TRiP RNAi stock info
· TRiP plasmid vectors
Mon. Oct. 18, 2021, 12 pm EST, Drosophila in vivo CRISPR technologies
[Missed it? View a summary info file here.]
[Missed it? View a summary info file here.]
· In vivo CRISPR knockout using TRiP fly stocks
· In vivo CRISPR activation using TRiP fly stocks
· RSVP Plus online resource for lookup of TRiP CRISPR stock info
· In vivo prime editing
Mon. Nov. 1, 2021, 12 pm EST, Drosophila bioinformatics resources
[Missed it? See our user-centric NAR database issue paper here and 'tools overview' page here.]
[Missed it? See our user-centric NAR database issue paper here and 'tools overview' page here.]
· Using DRSC online tools for building ‘gene lists’
· Using DRSC online tools for designing and identifying reagents
· Using DRSC online tools for data analysis and integration
· Using DRSC online tools for data mining
Mon. Nov. 8, 2021, 12 pm EST, Mosquito CRISPR cell technologies
[Missed it? View our mosquito cell tech page here]
[Missed it? View our mosquito cell tech page here]
· How we handle mosquito cell culture and media at the DRSC
· Mosquito cell transfection by lipofection or electroporation
· Modified mosquito cells for low- or high-throughput CRISPR modification
· Mosquito cell CRISPR knockout screening
Registration: [now closed]
See also: Cell-based assays, DRSC-BTRR, CRISPR gRNA design, Cell-based CRISPR, in vivo fly CRISPR, Cell-based RNAi, in vivo fly RNAi, CRISPR gRNA design, CRISPR genome editing, CRISPRa, CRISPRi, DRSC-BTRR, Data mining, Data visualization, Disease models, Disease-related studies, Genome-wide screen, Ortholog identification, RNAi reagent design, RNAi rescue, RNAi validation, Screening technologies, Aedes aegypti, Culex quinquefasciatus, Aedes albopictus, Anopheles gambiae, Drosophila (fly)