RNAi validation

Content tagged with RNAi validation

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Important new considerations for TRiP stock users

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A handful of recent papers and preprints have highlighted some important considerations for using TRiP shRNA and sgRNA stocks. First, the Weake lab at Purdue University discovered that TRiP stocks contain a previously uncharacterized loss-of-function...

DRSC/TRiP and DRSC-BTRR Office Hours

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New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...

New publications from the DRSC bioinformatics team

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The DRSC bioinformatics team, led by Dr. Claire Yanhui Hu, has recently published two new papers. One reports development of BioLitMine, an advanced literature mining resource. The other provides an overview of our online resources, which can be grouped...

Micropublication relevant to TRiP fly stocks

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Users of TRiP RNAi and sgRNA fly stocks take note: the Weake lab at Purdue University brought to our attention that some TRiP fly stocks carry a mutant allele of seveneless. Jonathan Zirin worked with Spencer Escobedo and Vikki Weake, as well as with...

Now Available: Our 2017 NAR database issue update

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Read about the DRSC/TRiP Functional Genomics Resources online tools and more in our 2017 Nucleic Acids Research database issue update publication. Tables 1 & 2 in particular are meant to give you a quick look at what online tools we offer and how they fit...

Publication describes TRiP resources

News
Liz Perkins and colleagues have published a paper describing the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School. The article, published in the November 1, 2015 issue of Genetics, details the TRiP production pipeline , reagents...

Bibliographic References tagged with RNAi validation

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Shue Chen, Leah Rosin, Gianluca Pegoraro, Nellie Moshkovich, Patrick Murphy, Guoyun Yu, and Elissa Lei. 2022. “NURF301 Contributes to Gypsy Chromatin Insulator-Mediated Nuclear Organization”. Nucleic Acids Res, 50, 14, Pp. 7906-24. doi:10.1093/nar/gkac600
Shue Chen, Leah Rosin, Gianluca Pegoraro, Nellie Moshkovich, Patrick Murphy, Guoyun Yu, and Elissa Lei. 2022. “NURF301 Contributes to Gypsy Chromatin Insulator-Mediated Nuclear Organization”. Nucleic Acids Res, 50, 14, Pp. 7906-24. doi:10.1093/nar/gkac600
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Chieh-Han Lu, Stephanie E. Mohr, Jr Robert V. Farese, and Tobias C. Walther. 2021. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets [NOTE: A Modified Final Version Was Published in Nat Cell Biol and Is Now Available]”
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Chieh-Han Lu, Stephanie E. Mohr, Jr Robert V. Farese, and Tobias C. Walther. 2021. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets [NOTE: A Modified Final Version Was Published in Nat Cell Biol and Is Now Available]”
Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila”. Insect Sci. doi:10.1111/1744-7917.12954
Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila”. Insect Sci. doi:10.1111/1744-7917.12954
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
Spencer E. Escobedo, Jonathan Zirin, and Vikki M. Weake. 2019. “TRiP Stocks Contain a Previously Uncharacterized Loss-of-Function Sevenless Allele”. MicroPublication Biology
Spencer E. Escobedo, Jonathan Zirin, and Vikki M. Weake. 2019. “TRiP Stocks Contain a Previously Uncharacterized Loss-of-Function Sevenless Allele”. MicroPublication Biology
Zanet, Benrabah, Li, Pélissier-Monier, Chanut-Delalande, Ronsin, Bellen, Payre, and Plaza. 2015. “Pri SORF Peptides Induce Selective Proteasome-Mediated Protein Processing”. Science, 349, 6254, Pp. 1356-8. doi:10.1126/science.aac5677
Zanet, Benrabah, Li, Pélissier-Monier, Chanut-Delalande, Ronsin, Bellen, Payre, and Plaza. 2015. “Pri SORF Peptides Induce Selective Proteasome-Mediated Protein Processing”. Science, 349, 6254, Pp. 1356-8. doi:10.1126/science.aac5677
Chen X and Xu L. 2016. “Genome-Wide RNAi Screening to Dissect the TGF-β Signal Transduction Pathway”. Methods in Molecular Biology
Chen X and Xu L. 2016. “Genome-Wide RNAi Screening to Dissect the TGF-β Signal Transduction Pathway”. Methods in Molecular Biology
Yanhui Hu, Aram Comjean, Charles Roesel, Arunachalam Vinayagam, Ian Flockhart, Jonathan Zirin, Lizabeth Perkins, Norbert Perrimon, and Stephanie Mohr. 2016. “FlyRNAi.Org—the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2017 Update”. Nucleic Acids Research
Yanhui Hu, Aram Comjean, Charles Roesel, Arunachalam Vinayagam, Ian Flockhart, Jonathan Zirin, Lizabeth Perkins, Norbert Perrimon, and Stephanie Mohr. 2016. “FlyRNAi.Org—the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2017 Update”. Nucleic Acids Research
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet
Arunachalam Vinayagam, Meghana Kulkarni, Richelle Sopko, Xiaoyun Sun, Yanhui Hu, Ankita Nand, Christians Villalta, Ahmadali Moghimi, Xuemei Yang, Stephanie Mohr, Pengyu Hong, John Asara, and Norbert Perrimon. 2016. “An Integrative Analysis of the InR PI3K Akt Network Identifies the Dynamic Response to Insulin Signaling”. Cell Reports, 16, 11, Pp. 3062-74
Arunachalam Vinayagam, Meghana Kulkarni, Richelle Sopko, Xiaoyun Sun, Yanhui Hu, Ankita Nand, Christians Villalta, Ahmadali Moghimi, Xuemei Yang, Stephanie Mohr, Pengyu Hong, John Asara, and Norbert Perrimon. 2016. “An Integrative Analysis of the InR PI3K Akt Network Identifies the Dynamic Response to Insulin Signaling”. Cell Reports, 16, 11, Pp. 3062-74