in vivo fly RNAi

Content tagged with in vivo fly RNAi

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Important new considerations for TRiP stock users

News
A handful of recent papers and preprints have highlighted some important considerations for using TRiP shRNA and sgRNA stocks. First, the Weake lab at Purdue University discovered that TRiP stocks contain a previously uncharacterized loss-of-function...

DRSC/TRiP and DRSC-BTRR Office Hours

News
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...

DRSC-Biomedical Technology Research Resource

News
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...

Micropublication relevant to TRiP fly stocks

News
Users of TRiP RNAi and sgRNA fly stocks take note: the Weake lab at Purdue University brought to our attention that some TRiP fly stocks carry a mutant allele of seveneless. Jonathan Zirin worked with Spencer Escobedo and Vikki Weake, as well as with...

DRSC & TRiP Workshop at ADRC

Event
The DRSC & TRiP will be hosting a workshop at the Annual Drosophila Research Conference in Philadelphia, PA. The workshop is scheduled for Friday, April 13th from 1:45 to 3:45 PM. Come hear from DRSC & TRiP leaders Norbert Perrimon, Jonathan Zirin...

New stocks added to the TRiP in vivo RNAi library

News
The TRiP has updated and curated our list of in vivo RNAi reagents for gene knockdown in fruitflies. To date, we have produced over 13,000 stocks for the benefit of the scientific community. Visit the in vivo RNAi fly stocks and vectors page to download...

DRSC & TRiP at ADRC 2017

Event
The DRSC & TRiP will be at the Annual Drosophila Research Conference (aka 'the fly meeting') in San Diego, CA, in March/April of 2017. Please feel free to contact us if you'd like to set up a meeting to discuss a screen project or other research need we...

Is it a hit? On mining our data sets.

News
The DRSC/TRiP-FGR's FlyRNAi database stores results from the many cell-based screens done since 2003 using DRSC Drosophila RNAi libraries. It also stores information about knockdown and phenotypes resulting from specific combinations of in vivo RNAi fly...

Publication describes TRiP resources

News
Liz Perkins and colleagues have published a paper describing the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School. The article, published in the November 1, 2015 issue of Genetics, details the TRiP production pipeline , reagents...

Bibliographic References tagged with in vivo fly RNAi

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Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila”. Insect Sci. doi:10.1111/1744-7917.12954
Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila”. Insect Sci. doi:10.1111/1744-7917.12954
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies”. Genetics. doi:10.1534/genetics.119.302964
Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies”. Genetics. doi:10.1534/genetics.119.302964
Michael Rotelli, Anna Bolling, Andrew Killion, Abraham Weinberg, Michael Dixon, and Brian Calvi. 2019. “An RNAi Screen for Genes Required for Growth of Wing Tissue”. G3 (Bethesda), 9, 10, Pp. 3087-3100. doi:10.1534/g3.119.400581
Michael Rotelli, Anna Bolling, Andrew Killion, Abraham Weinberg, Michael Dixon, and Brian Calvi. 2019. “An RNAi Screen for Genes Required for Growth of Wing Tissue”. G3 (Bethesda), 9, 10, Pp. 3087-3100. doi:10.1534/g3.119.400581
Stephanie E. Mohr and Norbert Perrimon. 2019. “Drosophila Melanogaster: A Simple System for Understanding Complexity.”. Dis Model Mech, 12, 10
Stephanie E. Mohr and Norbert Perrimon. 2019. “Drosophila Melanogaster: A Simple System for Understanding Complexity.”. Dis Model Mech, 12, 10
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet
Ian Flockhart, Matthew Booker, Yanhui Hu, Benjamin McElvany, Quentin Gilly, Bernard Mathey-Prevot, Norbert Perrimon, and Stephanie Mohr. 2012. “FlyRNAi.Org--the Database of the Drosophila RNAi Screening Center: 2012 Update.”. Nucleic Acids Res, 40, Database issue, Pp. D715-9. doi:10.1093/nar/gkr953
Ian Flockhart, Matthew Booker, Yanhui Hu, Benjamin McElvany, Quentin Gilly, Bernard Mathey-Prevot, Norbert Perrimon, and Stephanie Mohr. 2012. “FlyRNAi.Org--the Database of the Drosophila RNAi Screening Center: 2012 Update.”. Nucleic Acids Res, 40, Database issue, Pp. D715-9. doi:10.1093/nar/gkr953
Ian Flockhart, Matthew Booker, Amy Kiger, Michael Boutros, Susan Armknecht, Nadire Ramadan, Kris Richardson, Andrew Xu, Norbert Perrimon, and Bernard Mathey-Prevot. 2006. “FlyRNAi: The Drosophila RNAi Screening Center Database.”. Nucleic Acids Res, 34, Database issue, Pp. D489-94. doi:10.1093/nar/gkj114
Ian Flockhart, Matthew Booker, Amy Kiger, Michael Boutros, Susan Armknecht, Nadire Ramadan, Kris Richardson, Andrew Xu, Norbert Perrimon, and Bernard Mathey-Prevot. 2006. “FlyRNAi: The Drosophila RNAi Screening Center Database.”. Nucleic Acids Res, 34, Database issue, Pp. D489-94. doi:10.1093/nar/gkj114
Meghana Kulkarni, Matthew Booker, Serena Silver, Adam Friedman, Pengyu Hong, Norbert Perrimon, and Bernard Mathey-Prevot. 2006. “Evidence of Off-Target Effects Associated With Long DsRNAs in Drosophila Melanogaster Cell-Based Assays.”. Nat Methods, 3, 10, Pp. 833-8. doi:10.1038/nmeth935
Meghana Kulkarni, Matthew Booker, Serena Silver, Adam Friedman, Pengyu Hong, Norbert Perrimon, and Bernard Mathey-Prevot. 2006. “Evidence of Off-Target Effects Associated With Long DsRNAs in Drosophila Melanogaster Cell-Based Assays.”. Nat Methods, 3, 10, Pp. 833-8. doi:10.1038/nmeth935
Jian-Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker, Lizabeth Perkins, and Norbert Perrimon. 2008. “Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila Melanogaster.”. Nat Methods, 5, 1, Pp. 49-51. doi:10.1038/nmeth1146
Jian-Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker, Lizabeth Perkins, and Norbert Perrimon. 2008. “Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila Melanogaster.”. Nat Methods, 5, 1, Pp. 49-51. doi:10.1038/nmeth1146