At the DRSC-BTRR, we've been doing a lot of pooled-format CRISPR knockout screens in Drosophila cells. We're finding the results to be robust and reproducible. And best of all, the results have been informative, providing insights into diverse areas of biology.
Thinking about how to do CRISPR knockout screens in cells is a little different from thinking about how to do a genetic or RNAi screen in vivo or doing an arrayed-format RNAi screen. Read on for tips on how to design and carry out a screen. Notably, pooled-format screens do not require...
Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help.
DRSC/TRiP Functional Genomics Resources at Harvard Medical School
Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly stocks? Questions about our new protocols and resources for CRISPR mosquito cell lines? Pop into our Zoom office hours to say hello and get our expert input! Registration is required (see below).
The DRSC bioinformatics team, led by Dr. Claire Yanhui Hu, has recently published two new papers.
One reports development of BioLitMine, an advanced literature mining resource. The other provides an overview of our online resources, which can be grouped into reagent, gene, and data-focused resources.
Did you miss the presentations from Claire Hu and Jonathan Zirin at the June 2020 Boston Area Drosophila Meeting? No problem! The slides can be accessed from this post. Click the title above to view the whole post, then scroll down to access the PDFs. These presentations describe what's new and next in bioinformatics and in vivo technologies at the DRSC/TRiP. Feel free to reach out with questions. Interested in the BAD meeting? Info about the meeting can be found here. Read more about DRSC/TRiP presentations from June 2020 Boston Area Drosophila Meeting
Paralogs can be defined as related genes within a genome that are thought to arise from gene duplication events. Because paralogous proteins share amino acid identity, they can have redundant functions. But the picture is not necessarily so straightforward. Indeed, there are examples in which paralogous genes have distinct functions in some tissues, and overlapping functions in others.
The DRSC/TRiP is engaged in a project in collaboration with the Perrimon and Bellen labs to generate resources useful for the study of paralogous genes in Drosophila.
In adherance with Harvard Medical School's response to the COVID-19 pandemic, the DRSC/TRiP-FGR and DRSC-BTRR will be closing our doors by 5 pm on Wed. March 16.
Services will be unavailable for at least 6-8 weeks but leaders and staff will be reachable by email, and related resources are available at the BDSC and DGRC in Indiana, and at Addgene, and information remains available at this site.
We are focused right now on minimizing the impact of the shut-down on our activities. In the future, we expect to be able to make use of the time to help our research...
Learn about DRSC/TRiP activities focused on using CRISPR activation to develop human disease models in a recently published Q&A with our Director, Dr. S.E. Mohr, who will be a speaker this spring at an upcoming genome editing conference in Boston, MA.