The TRiP is excited to announce our new TRiP-CRISPR transgenic fly libraries for gene knockout and overexpression. Visit the sgRNA tracking site to download and search existing TRiP-CRISPR fly stocks by gene or stock ID to obtain information on sgRNA sequence, function, vector, injection site, and availablility.
Those of us lucky enough to be at the Annual Drosophila Research Conference this morning saw a great talk by Francois Payre about regulation of Shavenbaby by small ORFs. A genome-wide cell-based screen done at the DRSC by Emilie Benrabah identified the mechanism of regulation. As this exemplifies, cell screens can help identify key pathways and factors that can then be followed up with in vivo studies.
The DRSC/TRiP-FGR is pleased to announce that we recently updated our high-throughput, high-content imaging system. Through funds from NIH, HHMI, and the Harvard Medical School Tools & Technology program, we were able to get a
We have updated our CRISPR sgRNA design tool. The results and JBrowse display are now based on Drosophila melanogasterFlyBase genome assembly release 6. We have also added seed scores in addition to efficiency prediction scores and other values available on detailed views of sgRNA designs. Quick tips: start a search with a gene symbol or other identifier, use the
DIOPT 6.0 went live this week. Newly added features include results from eggNOG, bringing the total number of alrogithms incorporated in our integrated search tool to 14. In addition, you can now search for paralogs. To do this, choose the same species for input and output. Examples for fly-fly and human-human paralog searches are shown. As always, your feedback is welcome.
Read about the DRSC/TRiP Functional Genomics Resources online tools and more in our 2017 Nucleic Acids Research database issue update publication. Tables 1 & 2 in particular are meant to give you a quick look at what online tools we offer and how they fit with a variety of research goals.
We recently udpated our Gene List Annotation for Drosophila (GLAD) online resource. At GLAD you could already view the members of a gene list, such as genes grouped as members of a pathway, process, or sharing a functional domain. Now, you can also ask if a gene of interest is a member of a given group. Please see examples of the two ways to use GLAD below. As always, we welcome your feedback, including suggestions for changes or additions to the curated lists, or for addition of new
Our DIOPT ortholog search tool has been updated to include the option to search for orthologs of a gene in all other species included. So you can search with, for example, a fly gene, and see orthologs in human, mouse, rat, frog, worm, and yeast.
Click on the button "show summary of top scores" to see a heat map view of the top-scoring ortholog matches in other species to your query species. This feature helps you see quickly if a gene has been conserved across many species or is, for example, only found in vertebrates.
As always, our tool supports batch-mode searches (you can