Genome-wide screen

Content tagged with Genome-wide screen

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Entomological Society of America "Entomology 2024" meeting

Event
We've submitted abstracts and plan to participate in the Entomological Society of America's Entomology 2024 conference in Phoneix, AZ, this fall to connect with new audiences as we continue development of our CRISPR screening platform for screens in...

Virtual CRISPR Workshop 'at' ADRC 2023

Event
The DRSC is collaborating with other groups to present a virtual workshop on CRISPR technologies for Drosophila cells and in vivo as part of the GSA Annual Drosophila Research Conference 2023.

DRSC & DRTC Genetics and Genomics Workshop

Event
The DRSC is collaborating with the Drosophila Research and Training Center of Nigeria and the laboratory of Prof. Ross Cagan (University of Glasgow) to present an online workshop on Drosophila genomics and genetics.

DRSC/TRiP and DRSC-BTRR Office Hours

News
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...

DRSC-Biomedical Technology Research Resource

News
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...

Missed us at ADRC 2018? View our workshop slides!

News
Thank you to all those who attended our workshop at last week's Annual Drosophila Research Conference in Philadelphia, PA, USA. It was great to talk fly stocks, cell screens, and bioinformatics with the community. We are here to help and look forward to...

Pooled-format CRISPR screens in Drosophila cells

News
The DRSC/TRiP-FGR is pleased to support collaborations on pooled CRISPR screens using the method recently, reported in eLife by Viswanatha et al. (PDF download file below). From the abstract: "... Here, we developed a site-specific integration strategy...

DRSC at 17th annual World Preclinical Congress

Event
The DRSC/TRiP-FGR Director, Stephanie Mohr, will be co-teaching a short course on cell-based screening and single-cell studies, as well as co-moderating a discussion session, at the 17th annual World Preclinical Congress.

Bibliographic References tagged with Genome-wide screen

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Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells”. Elife, 12. doi:10.7554/eLife.85542
Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells”. Elife, 12. doi:10.7554/eLife.85542
Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7
Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7
Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430
Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Wei-Chun Tang, Chieh-Han Lu, Stephanie E. Mohr, Robert V. Farese, and Tobias C. Walther. 2022. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets”. Nature Cell Biol
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Wei-Chun Tang, Chieh-Han Lu, Stephanie E. Mohr, Robert V. Farese, and Tobias C. Walther. 2022. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets”. Nature Cell Biol
Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. BioRxiv
Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. BioRxiv
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Chieh-Han Lu, Stephanie E. Mohr, Jr Robert V. Farese, and Tobias C. Walther. 2021. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets [NOTE: A Modified Final Version Was Published in Nat Cell Biol and Is Now Available]”
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Chieh-Han Lu, Stephanie E. Mohr, Jr Robert V. Farese, and Tobias C. Walther. 2021. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets [NOTE: A Modified Final Version Was Published in Nat Cell Biol and Is Now Available]”
Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila”. Insect Sci. doi:10.1111/1744-7917.12954
Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila”. Insect Sci. doi:10.1111/1744-7917.12954
Raghuvir Viswanatha, Roderick Brathwaite, Yanhui Hu, Zhongchi Li, Jonathan Rodiger, Pierre Merckaert, Verena Chung, Stephanie Mohr, and Norbert Perrimon. 2019. “Pooled CRISPR Screens in Drosophila Cells”. Curr Protoc Mol Biol, 129, 1, Pp. e111. doi:10.1002/cpmb.111
Raghuvir Viswanatha, Roderick Brathwaite, Yanhui Hu, Zhongchi Li, Jonathan Rodiger, Pierre Merckaert, Verena Chung, Stephanie Mohr, and Norbert Perrimon. 2019. “Pooled CRISPR Screens in Drosophila Cells”. Curr Protoc Mol Biol, 129, 1, Pp. e111. doi:10.1002/cpmb.111