TRiP plasmid vector sets

TRiP vectors are distributed by the Drosophila Genome Resource Center (Bloomington, IL, USA).
Please see the TRiP in vivo RNAi approach for information on vector design. Features of the  vectors are listed in the downloadable pdf files. Additional information and updated plasmid vector maps and sequence are available at the DGRC website (see notes below).

June 2019 notes:

  • UPDATED VECTOR MAPS and sequence are available at the DGRC (Bloomington, IL, USA). The updated maps and sequence are based on full-plasmid NGS sequencing done at the DRSC/TRiP in the spring of 2019, just prior to submission of the vectors to the DGRC. Most vectors are LoxP-5xUAS-LoxP-5xUAS but two are LoxP-2xUAS-LoxP-5xUAS.
  • Also now available at the DGRC--a LexAop version of the VALIUM series vectors, generated by the R. Mann lab and full-plasmid NGS sequenced by the DRSC/TRiP in the spring of 2019, just prior to submission of the LexAop version vector to the DGRC.
Knockdown Vectors
pVALIUM10 (GU931382.2) map and sequence Cloning Protocol
pWALIUM10 (GU931388.2) map and sequence Cloning Protocol
pVALIUM1 (GU931383.2) map and sequence  
pVALIUM20 map and sequence Cloning Protocol
pVALIUM22 map and sequence Cloning Protocol
pWALIUM20 map and sequence Cloning Protocol
pWALIUM22 map and sequence Cloning Protocol
pW20-QUAST map and sequence  
Overexpression Vectors
pVALIUM10-roe (GU931386.2) map and sequence Cloning Protocol
pVALIUM10-moe (GU931387.2) map and sequence Cloning Protocol
pWALIUM10-roe map and sequence Cloning Protocol
pWALIUM10-moe (GU931385.2) map and sequence Cloning Protocol
Cloning Tools
mENTRY (GU931384.1) map and sequence Cloning Protocol
For vector-specific questions please contact JianQuan Ni (jni@genetics.med.harvard.edu).