@article {825331,
	title = {High-throughput RNAi screening in cultured cells: a user{\textquoteright}s guide.},
	journal = {Nat Rev Genet},
	volume = {7},
	year = {2006},
	month = {2006 May},
	pages = {373-84},
	abstract = {<p>RNA interference has re-energized the field of functional genomics by enabling genome-scale loss-of-function screens in cultured cells. Looking back on the lessons that have been learned from the first wave of technology developments and applications in this exciting field, we provide both a user{\textquoteright}s guide for newcomers to the field and a detailed examination of some more complex issues, particularly concerning optimization and quality control, for more advanced users. From a discussion of cell lines, screening paradigms, reagent types and read-out methodologies, we explore in particular the complexities of designing optimal controls and normalization strategies for these challenging but extremely powerful studies.</p>},
	keywords = {Animals, Automation, Cells, Cultured, drosophila melanogaster, Gene Expression Profiling, Gene Transfer Techniques, Humans, Models, Biological, Phenotype, Quality Control, RNA Interference, RNA, Small Interfering},
	issn = {1471-0056},
	doi = {10.1038/nrg1836},
	author = {Echeverri, Christophe J and Perrimon, Norbert}
}
