Those of us lucky enough to be at the Annual Drosophila Research Conference this morning saw a great talk by Francois Payre about regulation of Shavenbaby by small ORFs. A genome-wide cell-based screen done at the DRSC by Emilie Benrabah identified the mechanism of regulation. As this exemplifies, cell screens can help identify key pathways and factors that can then be followed up with in vivo studies.
The TRiP has updated and curated our list of in vivo RNAi reagents for gene knockdown in fruitflies.To date, we have produced over 13,000 stocks for the benefit of the scientific community.
Visit the in vivo RNAi fly stocks and vectors page to download an excel file with the full list of fly stocks now available for order from the BloomingtonDrosophila Stock Center (BDSC) and the National Institute of Genetics, Japan (NIG-Fly).
Read about the DRSC/TRiP Functional Genomics Resources online tools and more in our 2017 Nucleic Acids Research database issue update publication. Tables 1 & 2 in particular are meant to give you a quick look at what online tools we offer and how they fit with a variety of research goals.
Our DIOPT ortholog search tool has been updated to include the option to search for orthologs of a gene in all other species included. So you can search with, for example, a fly gene, and see orthologs in human, mouse, rat, frog, worm, and yeast.
The DRSC/TRiP-FGR's FlyRNAi database stores results from the many cell-based screens done since 2003 using DRSC Drosophila RNAi libraries. It also stores information about knockdown and phenotypes resulting from specific combinations of in vivo RNAi fly stocks (including our TRiP stocks and also VDRC and NIG-Japan stocks). The in vivo data includes directly deposited data and results curated by FlyBase from the literature. Read more about Is it a hit? On mining our data sets.
Liz Perkins and colleagues have published a paper describing the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School. The article, published in the November 1, 2015 issue of Genetics, details the TRiP production pipeline, reagents generated, state of the collection, and validation efforts.
This is a great introduction to the many in vivo RNAi resources the DRSC/TRiP-FGR provides to the scientific community.