For activation of target gene expression
TRiP-OE: The flySAM2.0 Collection
- New simplified strategy for in vivo CRISPR activation
- flySAM generates stronger overexpression phenotypes, comparable to Gal4/UAS
- Activate expression of target gene by crossing to any Gal4 line
TRiP-OE: The VPR Collection
- Activate expression of target gene by crossing to Gal4>dCas9-VPR flies
- Generate InDels and larger genomic deletions by crossing to Gal4>Cas9 flies
- For a detailed description of the the TRiP-OE gene activation approach click here
About TRiP-OE stocks
flySAM
- A single gene is targeted by expression of one sgRNA from the U6:2 promoter
- Stocks are made in the flySAM2.0 vector, developed by Jian-quan Ni and colleagues
- TRiP-OE/flySAM stocks contain UAS-Cas9, so simply crossing to a Gal4 induces expression of the target gene
- To learn more about the TRiP-OE vectors, sgRNA design, and gene activation please visit the in vivo CRISPR protocols page
VPR
- A single gene is targeted by tandem expression of two sgRNAs from independent U6 promoters
- Stocks are made in the pCFD4 vector, developed by Fillip Port and colleagues (http://www.crisprflydesign.org/plasmids)
- Crossing TRiP-OE stocks to a Gal4 line expressing dCas9 fused to the chimeric activator domain VPR induces expression of the target gene
- To learn more about the TRiP-OE vectors, sgRNA design, and gene activation please visit the in vivo CRISPR protocols page
Access TRiP-OE stocks
- Order TRiP-OE transgenic stocks at the Bloomington Drosophila Stock Center (BDSC)
- If a TRiP-CRISPR stock has not yet been deposited at the BDSC, please contact the TRiP facility at HMS and we will send it to you
- Download a .XLS file of currently available TRiP-CRISPR stocks
Search TRiP-OE stocks
- Search the DRSC/TRiP sgRNA Stock Tracking System for TRiP-OE stocks and production status, and nominate genes for production