DRSC/TRiP Functional Genomics Resources & DRSC-BTRR

DRSC

The DRSC supports Drosophila cell screens using RNAi or CRISPR, generates new cell lines, and applies CRISPR screen tech to mosquito and other arthropod cell lines.

GFP-tagged and newly isolated Drosophila cell lines generated in the project funded by R24 OD019847 (to Perrimon, Mohr, and Simcox), showing GFP localized to specific organelles and example tissue-type-specific cell lines such as a muscle-like cell line

TRiP

The TRiP generates RNAi and CRISPR fly stocks for gene knockdown, knockout, and over-expression, and innovates in related areas of new in vivo resource development.

Top of a vial of Drosophila showning adult flies; the TRiP has generated many transgenic Drosophila fly stocks for knockdown, knockout, binary expression, and other uses

Online Tools

Our bioinformatics suite includes tools for identifying genes, orthologs, and reagents; analyzing, visualizing, and mining datasets; and more.

Screenshot of a grid layout of icons and links to our suite of online tools, used to demonstrate that we have a large suite of available online tools

Please Note: This website is now considered a legacy website and is not regularly updated. 

We invite you to view our new Arthropod Cell Screening Facility (ACSF) website for information about current initiatives and goals, current publication lists, and more. We will retain this site as long as feasible so that the community continues to have access to DRSC protocols, TRiP info, and more.

The Drosophila RNAi Screening Center (DRSC) & Transgenic RNAi Project (TRiP)-Functional Genomics Resources (DRSC/TRiP-FGR) at Harvard Medical School, also known as the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR), has been developing technology and resources for the Drosophila and broader research community since 2004.

Recent News

Nanobodies against NanoTags now easier to access
The nanobodies recognizing the VHH05-tag and 127D01-tag, two short epitope tags developed by our group for use in Drosophila , are now available in purified form from Cell Signaling Technology (CST). These "NanoTags" and their corresponding high-affinity...

PANGEA online tool updated
We recently updated our PANGEA gene set enrichment analysis tool. The tool now supports gene set enrichment analysis for the malaria mosquito Anopheles gambiae and the black-legged tick Ixodes scapularis. In addition, we have added new gene sets for gene...

DIOPT online ortholog search tool updated
We recently updated our DIOPT online ortholog search tool. Notable changes include addition of predictions for the black-legged tick, Ixodes scapularis, and removal of an outdated ortholog prediction set. Please see the attached PDF for a visual summary...
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Recent Preprints & Publications

2025

Raghuvir Viswanatha, Samuel Entwisle, Yanhui Hu, Ah-Ram Kim, Kelly Reap, Matthew Butnaru, Mujeeb Qadiri, Stephanie E Mohr, and Norbert Perrimon. 2025. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. Nature Communications, 16, 1, Pp. 6498. doi:10.1038/s41467-025-61692-3
Raghuvir Viswanatha, Samuel Entwisle, Yanhui Hu, Ah-Ram Kim, Kelly Reap, Matthew Butnaru, Mujeeb Qadiri, Stephanie E Mohr, and Norbert Perrimon. 2025. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. Nature Communications, 16, 1, Pp. 6498. doi:10.1038/s41467-025-61692-3
Yanhui Hu, Jonathan Rodiger, Yifang Liu, Chenxi Gao, Ying Liu, Mujeeb Qadiri, Austin Veal, Martha L Bulyk, and Norbert Perrimon. 2025. “TF2TG: An Online Resource Mining the Potential Gene Targets of Transcription Factors in Drosophila.”. Genetics. doi:10.1093/genetics/iyaf082
Yanhui Hu, Jonathan Rodiger, Yifang Liu, Chenxi Gao, Ying Liu, Mujeeb Qadiri, Austin Veal, Martha L Bulyk, and Norbert Perrimon. 2025. “TF2TG: An Online Resource Mining the Potential Gene Targets of Transcription Factors in Drosophila.”. Genetics. doi:10.1093/genetics/iyaf082
Alejandra Wu-Chuang, Hanna J Laukaitis-Yousey, Matthew Butnaru, Stephanie E Mohr, Norbert Perrimon, and Joao H F Pedra. 2025. “Decoding Arthropod Vector Immunology through Bona Fide Pathogens.”. Trends in Parasitology, 41, 5, Pp. 351-60. doi:10.1016/j.pt.2025.03.004
Alejandra Wu-Chuang, Hanna J Laukaitis-Yousey, Matthew Butnaru, Stephanie E Mohr, Norbert Perrimon, and Joao H F Pedra. 2025. “Decoding Arthropod Vector Immunology through Bona Fide Pathogens.”. Trends in Parasitology, 41, 5, Pp. 351-60. doi:10.1016/j.pt.2025.03.004
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