Cell-based RNAi (DRSC)
Quick link to Gene Lookup (search screen data)
Quick link to UP-TORR (view cell & in vivo reagents)
Drosophila cell-based RNAi screens have been supported by our facility since 2003. We provide reagents targeting individual genes, focused libraries, genome-scale libraries, and other resources for on-site screening at our facility or off-site screening at your institution.
- view our genome-wide and focused libraries (reagents)
- learn about making RNAi reagents or fly cell culture (protocols)
- mine data from past screens at ScreenSummary
See links below to relevant online tools, reagents, protocols, publications, and more.
News
Read the DRSC/TRiP Report to the Fly Board 2022
Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help. DRSC/TRiP Functional Genomics Resources at...
DRSC/TRiP and DRSC-BTRR Office Hours
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...
Contact Us
Please contact us for any questions.
Publications
2022
Shue Chen, Leah Rosin, Gianluca Pegoraro, Nellie Moshkovich, Patrick Murphy, Guoyun Yu, and Elissa Lei. 2022. “NURF301 Contributes to Gypsy Chromatin Insulator-Mediated Nuclear Organization”. Nucleic Acids Res, 50, 14, Pp. 7906-24. doi:10.1093/nar/gkac600
Shue Chen, Leah Rosin, Gianluca Pegoraro, Nellie Moshkovich, Patrick Murphy, Guoyun Yu, and Elissa Lei. 2022. “NURF301 Contributes to Gypsy Chromatin Insulator-Mediated Nuclear Organization”. Nucleic Acids Res, 50, 14, Pp. 7906-24. doi:10.1093/nar/gkac600
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Wei-Chun Tang, Chieh-Han Lu, Stephanie E. Mohr, Robert V. Farese, and Tobias C. Walther. 2022. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets”. Nature Cell Biol
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Wei-Chun Tang, Chieh-Han Lu, Stephanie E. Mohr, Robert V. Farese, and Tobias C. Walther. 2022. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets”. Nature Cell Biol
2021
Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila”. Insect Sci. doi:10.1111/1744-7917.12954
Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila”. Insect Sci. doi:10.1111/1744-7917.12954
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Chieh-Han Lu, Stephanie E. Mohr, Jr Robert V. Farese, and Tobias C. Walther. 2021. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets [NOTE: A Modified Final Version Was Published in Nat Cell Biol and Is Now Available]”
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Chieh-Han Lu, Stephanie E. Mohr, Jr Robert V. Farese, and Tobias C. Walther. 2021. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets [NOTE: A Modified Final Version Was Published in Nat Cell Biol and Is Now Available]”
2020
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
2019
Hilary Nicholson, Zeshan Tariq, Benjamin Housden, Rebecca Jennings, Laura Stransky, Norbert Perrimon, Sabina Signoretti, and William Kaelin. 2019. “HIF-Independent Synthetic Lethality Between CDK4 6 Inhibition and VHL Loss across Species”. Sci Signal, 12, 601. doi:10.1126/scisignal.aay0482
Hilary Nicholson, Zeshan Tariq, Benjamin Housden, Rebecca Jennings, Laura Stransky, Norbert Perrimon, Sabina Signoretti, and William Kaelin. 2019. “HIF-Independent Synthetic Lethality Between CDK4 6 Inhibition and VHL Loss across Species”. Sci Signal, 12, 601. doi:10.1126/scisignal.aay0482