URL
http://www.flyrnai.org/tools/dget/web
DGET- Drosophila Gene Expression Tool
DGET Overview
DGET was designed to facilitate the retrieval and analysis of RNA-Seq based expression data in a high-throughput fashion. The tool can be compared with expression features at FlyMine and FlyBase. FlyMine provides array-based expression data for high-throughput analysis. FlyBase provides both array-base and RNA-Seq based expression data one gene a time. As a supplement to FlyMine and FlyBase, DGET allows batch searches, filtration and enrichment analyses for a list of genes. In addition, at DGET users can search for genes with similar or opposite expression profiles and can build a synexpression network using any of the datasets.
Cite DGET
A preprint version of the DGET manuscript is available at http://biorxiv.org/content/early/2016/09/15/075358
Datasets currently covered at DGET
Cherbas, L. et al (2011). "The transcriptional diversity of 25 Drosophila cell lines." Genome Res 21(2): 301-314.
Graveley, B. R. et al (2011). "The developmental transcriptome of Drosophila melanogaster." Nature 471(7339): 473-479.
Graveley, B. R. et al (2011). "The developmental transcriptome of Drosophila melanogaster." Nature 471(7339): 473-479.
Graveley, B. R. et al (2011). "The developmental transcriptome of Drosophila melanogaster." Nature 471(7339): 473-479.
Marianes, A. and A. C. Spradling (2013). "Physiological and stem cell compartmentalization within the Drosophila midgut." Elife 2: e00886.
Dutta, D. et al (2015). "Regional Cell-Specific Transcriptome Mapping Reveals Regulatory Complexity in the Adult Drosophila Midgut." Cell Rep 12(2): 346-358.
Data visualization
- Heatmap view
Plotting of heat-maps for svg download is done in R using the gplot heatmap function.
- Network view
Plotting of network is done using Cytoscape and a JSON representation of the network is sent with the html. The nodes are input genes while the edges are drawn for gene pairs with Pearson correlation score that meets user defined cut-off
Please use our bug report form to ask questions or make suggestions.