Cell-based assays
Regardless of the technology (RNAi, CRISPR, over-expression, etc.), a good cell-based assay is the best foundation for a cell-based screen. We have equipment, provide reagents, share protocols, and more to support development of high-throughput screen assays in Drosophila cells.
Reagents, consultation, and other support is available for screens off-site. We also support screens on-site at our facility. Assays can be done using a number of types of reagents, including reagents for knockdown or over-expression of protein-coding genes, and interrogation of miRNAs.
See links below to relevant reagents, protocols, publications, and more.
News
New cell lines & new understandings using cutting-edge techniques
As a facility that supports large-scale screens in Drosophila and other insect cell lines, we get excited about reports of new Drosophila cell lines and related info. We'd like to highlight two recent papers. One report, a collaboration between Amanda...
So you want to do a CRISPR pooled screen in insect cells? You can! Here's how
At the DRSC-BTRR, we've been doing a lot of pooled-format CRISPR knockout screens in Drosophila cells. We're finding the results to be robust and reproducible. And best of all, the results have been informative, providing insights into diverse areas of...
Read the DRSC/TRiP Report to the Fly Board 2022
Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help. DRSC/TRiP Functional Genomics Resources at...
DRSC/TRiP and DRSC-BTRR Office Hours
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...
DRSC-Biomedical Technology Research Resource
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...
Drosophila cell screen with DRSC reagent library contributes to identification of new therapeutic target for renal cancer
We here at the DRSC/TRiP are thrilled to see this study from Hilary Nicholson et al. published in Science Signaling. The study provides a great example of how screens in Drosophila cultured cells can be used as part of a cross-species platform aimed at...
Contact Us
Please contact us for any questions.
Publications
2012
Ian Flockhart, Matthew Booker, Yanhui Hu, Benjamin McElvany, Quentin Gilly, Bernard Mathey-Prevot, Norbert Perrimon, and Stephanie Mohr. 2012. “FlyRNAi.Org--the Database of the Drosophila RNAi Screening Center: 2012 Update.”. Nucleic Acids Res, 40, Database issue, Pp. D715-9. doi:10.1093/nar/gkr953
Ian Flockhart, Matthew Booker, Yanhui Hu, Benjamin McElvany, Quentin Gilly, Bernard Mathey-Prevot, Norbert Perrimon, and Stephanie Mohr. 2012. “FlyRNAi.Org--the Database of the Drosophila RNAi Screening Center: 2012 Update.”. Nucleic Acids Res, 40, Database issue, Pp. D715-9. doi:10.1093/nar/gkr953
Reid Aikin, Alexandra Cervantes, Gisela D’Angelo, Laurent Ruel, Sandra Lacas-Gervais, Sébastien Schaub, and Pascal Thérond. 2012. “A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila.”. PLoS One, 7, 3, Pp. e33665. doi:10.1371/journal.pone.0033665
Reid Aikin, Alexandra Cervantes, Gisela D’Angelo, Laurent Ruel, Sandra Lacas-Gervais, Sébastien Schaub, and Pascal Thérond. 2012. “A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila.”. PLoS One, 7, 3, Pp. e33665. doi:10.1371/journal.pone.0033665
Artyom Alekseyenko, Joshua Ho, Shouyong Peng, Marnie Gelbart, Michael Tolstorukov, Annette Plachetka, Peter Kharchenko, Youngsook Jung, Andrey Gorchakov, Erica Larschan, Tingting Gu, Aki Minoda, Nicole Riddle, Yuri Schwartz, Sarah Elgin, Gary Karpen, Vincenzo Pirrotta, Mitzi Kuroda, and Peter Park. 2012. “Sequence-Specific Targeting of Dosage Compensation in Drosophila Favors an Active Chromatin Context.”. PLoS Genet, 8, 4, Pp. e1002646. doi:10.1371/journal.pgen.1002646
Artyom Alekseyenko, Joshua Ho, Shouyong Peng, Marnie Gelbart, Michael Tolstorukov, Annette Plachetka, Peter Kharchenko, Youngsook Jung, Andrey Gorchakov, Erica Larschan, Tingting Gu, Aki Minoda, Nicole Riddle, Yuri Schwartz, Sarah Elgin, Gary Karpen, Vincenzo Pirrotta, Mitzi Kuroda, and Peter Park. 2012. “Sequence-Specific Targeting of Dosage Compensation in Drosophila Favors an Active Chromatin Context.”. PLoS Genet, 8, 4, Pp. e1002646. doi:10.1371/journal.pgen.1002646
Eric Joyce, Benjamin Williams, Tiao Xie, and C-Ting Wu. 2012. “Identification of Genes That Promote or Antagonize Somatic Homolog Pairing Using a High-Throughput FISH-Based Screen.”. PLoS Genet, 8, 5, Pp. e1002667. doi:10.1371/journal.pgen.1002667
Eric Joyce, Benjamin Williams, Tiao Xie, and C-Ting Wu. 2012. “Identification of Genes That Promote or Antagonize Somatic Homolog Pairing Using a High-Throughput FISH-Based Screen.”. PLoS Genet, 8, 5, Pp. e1002667. doi:10.1371/journal.pgen.1002667
Erica Larschan, Marcela Soruco, Ok-Kyung Lee, Shouyong Peng, Eric Bishop, Jessica Chery, Karen Goebel, Jessica Feng, Peter Park, and Mitzi Kuroda. 2012. “Identification of Chromatin-Associated Regulators of MSL Complex Targeting in Drosophila Dosage Compensation.”. PLoS Genet, 8, 7, Pp. e1002830. doi:10.1371/journal.pgen.1002830
Erica Larschan, Marcela Soruco, Ok-Kyung Lee, Shouyong Peng, Eric Bishop, Jessica Chery, Karen Goebel, Jessica Feng, Peter Park, and Mitzi Kuroda. 2012. “Identification of Chromatin-Associated Regulators of MSL Complex Targeting in Drosophila Dosage Compensation.”. PLoS Genet, 8, 7, Pp. e1002830. doi:10.1371/journal.pgen.1002830
Stephanie Stotz and David Clapham. 2012. “Anion-Sensitive Fluorophore Identifies the Drosophila Swell-Activated Chloride Channel in a Genome-Wide RNA Interference Screen.”. PLoS One, 7, 10, Pp. e46865. doi:10.1371/journal.pone.0046865
Stephanie Stotz and David Clapham. 2012. “Anion-Sensitive Fluorophore Identifies the Drosophila Swell-Activated Chloride Channel in a Genome-Wide RNA Interference Screen.”. PLoS One, 7, 10, Pp. e46865. doi:10.1371/journal.pone.0046865