Cell-based assays
Regardless of the technology (RNAi, CRISPR, over-expression, etc.), a good cell-based assay is the best foundation for a cell-based screen. We have equipment, provide reagents, share protocols, and more to support development of high-throughput screen assays in Drosophila cells.
Reagents, consultation, and other support is available for screens off-site. We also support screens on-site at our facility. Assays can be done using a number of types of reagents, including reagents for knockdown or over-expression of protein-coding genes, and interrogation of miRNAs.
See links below to relevant reagents, protocols, publications, and more.
News
New cell lines & new understandings using cutting-edge techniques
As a facility that supports large-scale screens in Drosophila and other insect cell lines, we get excited about reports of new Drosophila cell lines and related info. We'd like to highlight two recent papers. One report, a collaboration between Amanda...
So you want to do a CRISPR pooled screen in insect cells? You can! Here's how
At the DRSC-BTRR, we've been doing a lot of pooled-format CRISPR knockout screens in Drosophila cells. We're finding the results to be robust and reproducible. And best of all, the results have been informative, providing insights into diverse areas of...
Read the DRSC/TRiP Report to the Fly Board 2022
Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help. DRSC/TRiP Functional Genomics Resources at...
DRSC/TRiP and DRSC-BTRR Office Hours
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...
DRSC-Biomedical Technology Research Resource
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...
Drosophila cell screen with DRSC reagent library contributes to identification of new therapeutic target for renal cancer
We here at the DRSC/TRiP are thrilled to see this study from Hilary Nicholson et al. published in Science Signaling. The study provides a great example of how screens in Drosophila cultured cells can be used as part of a cross-species platform aimed at...
Contact Us
Please contact us for any questions.
Publications
2013
Felix Muerdter, Paloma Guzzardo, Jesse Gillis, Yicheng Luo, Yang Yu, Caifu Chen, Richard Fekete, and Gregory Hannon. 2013. “A Genome-Wide RNAi Screen Draws a Genetic Framework for Transposon Control and Primary PiRNA Biogenesis in Drosophila.”. Mol Cell, 50, 5, Pp. 736-48. doi:10.1016/j.molcel.2013.04.006
Felix Muerdter, Paloma Guzzardo, Jesse Gillis, Yicheng Luo, Yang Yu, Caifu Chen, Richard Fekete, and Gregory Hannon. 2013. “A Genome-Wide RNAi Screen Draws a Genetic Framework for Transposon Control and Primary PiRNA Biogenesis in Drosophila.”. Mol Cell, 50, 5, Pp. 736-48. doi:10.1016/j.molcel.2013.04.006
Keren Imberg-Kazdan, Susan Ha, Alex Greenfield, Christopher Poultney, Richard Bonneau, Susan Logan, and Michael Garabedian. 2013. “A Genome-Wide RNA Interference Screen Identifies New Regulators of Androgen Receptor Function in Prostate Cancer Cells.”. Genome Res, 23, 4, Pp. 581-91. doi:10.1101/gr.144774.112
Keren Imberg-Kazdan, Susan Ha, Alex Greenfield, Christopher Poultney, Richard Bonneau, Susan Logan, and Michael Garabedian. 2013. “A Genome-Wide RNA Interference Screen Identifies New Regulators of Androgen Receptor Function in Prostate Cancer Cells.”. Genome Res, 23, 4, Pp. 581-91. doi:10.1101/gr.144774.112
Clément Carré, Caroline Jacquier, Anne-Laure Bougé, Fabrice Chaumont, Corinne Besnard-Guerin, Hélène Thomassin, Josette Pidoux, Bruno Da Silva, Eleftheria Chalatsi, Sarah Zahra, Jean-Christophe Olivo-Marin, Hélène Munier-Lehmann, and Christophe Antoniewski. 2013. “AutomiG, a Biosensor to Detect Alterations in MiRNA Biogenesis and in Small RNA Silencing Guided by Perfect Target Complementarity.”. PLoS One, 8, 9, Pp. e74296. doi:10.1371/journal.pone.0074296
Clément Carré, Caroline Jacquier, Anne-Laure Bougé, Fabrice Chaumont, Corinne Besnard-Guerin, Hélène Thomassin, Josette Pidoux, Bruno Da Silva, Eleftheria Chalatsi, Sarah Zahra, Jean-Christophe Olivo-Marin, Hélène Munier-Lehmann, and Christophe Antoniewski. 2013. “AutomiG, a Biosensor to Detect Alterations in MiRNA Biogenesis and in Small RNA Silencing Guided by Perfect Target Complementarity.”. PLoS One, 8, 9, Pp. e74296. doi:10.1371/journal.pone.0074296
Clemens Bergwitz, Mark Wee, Sumi Sinha, Joanne Huang, Charles DeRobertis, Lawrence Mensah, Jonathan Cohen, Adam Friedman, Meghana Kulkarni, Yanhui Hu, Arunachalam Vinayagam, Michael Schnall-Levin, Bonnie Berger, Lizabeth Perkins, Stephanie Mohr, and Norbert Perrimon. 2013. “Genetic Determinants of Phosphate Response in Drosophila.”. PLoS One, 8, 3, Pp. e56753. doi:10.1371/journal.pone.0056753
Clemens Bergwitz, Mark Wee, Sumi Sinha, Joanne Huang, Charles DeRobertis, Lawrence Mensah, Jonathan Cohen, Adam Friedman, Meghana Kulkarni, Yanhui Hu, Arunachalam Vinayagam, Michael Schnall-Levin, Bonnie Berger, Lizabeth Perkins, Stephanie Mohr, and Norbert Perrimon. 2013. “Genetic Determinants of Phosphate Response in Drosophila.”. PLoS One, 8, 3, Pp. e56753. doi:10.1371/journal.pone.0056753
2012
Mar Arias Garcia, Miguel Sanchez Alvarez, Heba Sailem, Vicky Bousgouni, Julia Sero, and Chris Bakal. 2012. “Differential RNAi Screening Provides Insights into the Rewiring of Signalling Networks During Oxidative Stress.”. Mol Biosyst, 8, 10, Pp. 2605-13. doi:10.1039/c2mb25092f
Mar Arias Garcia, Miguel Sanchez Alvarez, Heba Sailem, Vicky Bousgouni, Julia Sero, and Chris Bakal. 2012. “Differential RNAi Screening Provides Insights into the Rewiring of Signalling Networks During Oxidative Stress.”. Mol Biosyst, 8, 10, Pp. 2605-13. doi:10.1039/c2mb25092f
Joseph Dopie, Kari-Pekka Skarp, Eeva Kaisa Rajakylä, Kimmo Tanhuanpää, and Maria Vartiainen. 2012. “Active Maintenance of Nuclear Actin by Importin 9 Supports Transcription.”. Proc Natl Acad Sci U S A, 109, 9, Pp. E544-52. doi:10.1073/pnas.1118880109
Joseph Dopie, Kari-Pekka Skarp, Eeva Kaisa Rajakylä, Kimmo Tanhuanpää, and Maria Vartiainen. 2012. “Active Maintenance of Nuclear Actin by Importin 9 Supports Transcription.”. Proc Natl Acad Sci U S A, 109, 9, Pp. E544-52. doi:10.1073/pnas.1118880109