Cell-based assays
Regardless of the technology (RNAi, CRISPR, over-expression, etc.), a good cell-based assay is the best foundation for a cell-based screen. We have equipment, provide reagents, share protocols, and more to support development of high-throughput screen assays in Drosophila cells.
Reagents, consultation, and other support is available for screens off-site. We also support screens on-site at our facility. Assays can be done using a number of types of reagents, including reagents for knockdown or over-expression of protein-coding genes, and interrogation of miRNAs.
See links below to relevant reagents, protocols, publications, and more.
News
New cell lines & new understandings using cutting-edge techniques
As a facility that supports large-scale screens in Drosophila and other insect cell lines, we get excited about reports of new Drosophila cell lines and related info. We'd like to highlight two recent papers. One report, a collaboration between Amanda...
So you want to do a CRISPR pooled screen in insect cells? You can! Here's how
At the DRSC-BTRR, we've been doing a lot of pooled-format CRISPR knockout screens in Drosophila cells. We're finding the results to be robust and reproducible. And best of all, the results have been informative, providing insights into diverse areas of...
Read the DRSC/TRiP Report to the Fly Board 2022
Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help. DRSC/TRiP Functional Genomics Resources at...
DRSC/TRiP and DRSC-BTRR Office Hours
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...
DRSC-Biomedical Technology Research Resource
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...
Drosophila cell screen with DRSC reagent library contributes to identification of new therapeutic target for renal cancer
We here at the DRSC/TRiP are thrilled to see this study from Hilary Nicholson et al. published in Science Signaling. The study provides a great example of how screens in Drosophila cultured cells can be used as part of a cross-species platform aimed at...
Contact Us
Please contact us for any questions.
Publications
2005
Ramanuj DasGupta, Ajamete Kaykas, Randall Moon, and Norbert Perrimon. 2005. “Functional Genomic Analysis of the Wnt-Wingless Signaling Pathway.”. Science, 308, 5723, Pp. 826-33. doi:10.1126/science.1109374
Ramanuj DasGupta, Ajamete Kaykas, Randall Moon, and Norbert Perrimon. 2005. “Functional Genomic Analysis of the Wnt-Wingless Signaling Pathway.”. Science, 308, 5723, Pp. 826-33. doi:10.1126/science.1109374
Sara Cherry, Tammy Doukas, Susan Armknecht, Sean Whelan, Hui Wang, Peter Sarnow, and Norbert Perrimon. 2005. “Genome-Wide RNAi Screen Reveals a Specific Sensitivity of IRES-Containing RNA Viruses to Host Translation Inhibition.”. Genes Dev, 19, 4, Pp. 445-52. doi:10.1101/gad.1267905
Sara Cherry, Tammy Doukas, Susan Armknecht, Sean Whelan, Hui Wang, Peter Sarnow, and Norbert Perrimon. 2005. “Genome-Wide RNAi Screen Reveals a Specific Sensitivity of IRES-Containing RNA Viruses to Host Translation Inhibition.”. Genes Dev, 19, 4, Pp. 445-52. doi:10.1101/gad.1267905
Susan Armknecht, Michael Boutros, Amy Kiger, Kent Nybakken, Bernard Mathey-Prevot, and Norbert Perrimon. 2005. “High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells.”. Methods Enzymol, 392, Pp. 55-73. doi:10.1016/S0076-6879(04)92004-6
Susan Armknecht, Michael Boutros, Amy Kiger, Kent Nybakken, Bernard Mathey-Prevot, and Norbert Perrimon. 2005. “High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells.”. Methods Enzymol, 392, Pp. 55-73. doi:10.1016/S0076-6879(04)92004-6
2004
Ulrike Eggert, Amy Kiger, Constance Richter, Zachary Perlman, Norbert Perrimon, Timothy Mitchison, and Christine Field. 2004. “Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets.”. PLoS Biol, 2, 12, Pp. e379. doi:10.1371/journal.pbio.0020379
Ulrike Eggert, Amy Kiger, Constance Richter, Zachary Perlman, Norbert Perrimon, Timothy Mitchison, and Christine Field. 2004. “Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets.”. PLoS Biol, 2, 12, Pp. e379. doi:10.1371/journal.pbio.0020379
Michael Boutros, Amy Kiger, Susan Armknecht, Kim Kerr, Marc Hild, Britta Koch, Stefan Haas, Renato Paro, Norbert Perrimon, and Heidelberg Fly Array Consortium. 2004. “Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells.”. Science, 303, 5659, Pp. 832-5. doi:10.1126/science.1091266
Michael Boutros, Amy Kiger, Susan Armknecht, Kim Kerr, Marc Hild, Britta Koch, Stefan Haas, Renato Paro, Norbert Perrimon, and Heidelberg Fly Array Consortium. 2004. “Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells.”. Science, 303, 5659, Pp. 832-5. doi:10.1126/science.1091266
2003
Kiger, Baum, Jones, Jones, Coulson, Echeverri, and Perrimon. 2003. “A Functional Genomic Analysis of Cell Morphology Using RNA Interference.”. J Biol, 2, 4, Pp. 27. doi:10.1186/1475-4924-2-27
Kiger, Baum, Jones, Jones, Coulson, Echeverri, and Perrimon. 2003. “A Functional Genomic Analysis of Cell Morphology Using RNA Interference.”. J Biol, 2, 4, Pp. 27. doi:10.1186/1475-4924-2-27