Genome-wide RNAi analysis of growth and viability in Drosophila cells.


Michael Boutros, Amy A Kiger, Susan Armknecht, Kim Kerr, Marc Hild, Britta Koch, Stefan A Haas, Renato Paro, Norbert Perrimon, and Norbert Perrimon. 2004. “Genome-wide RNAi analysis of growth and viability in Drosophila cells.” Science, 303, 5659, Pp. 832-5.
2004_Science_Boutros.pdf536 KB


A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale.

Last updated on 08/31/2016