The DRSC/TRiP-FGR is pleased to support collaborations on pooled CRISPR screens using the method recently reported in a BioRxiv pre-print by Viswanatha et al. From the abstract: "... Here, we developed a site-specific integration strategy for library delivery and performed a genome-wide CRISPR knockout screen in Drosophila S2R+ cells. Under basal growth conditions, 1235 genes were essential for cell fitness at a false-discovery rate of 5% ... We additionally performed context-specific fitness screens for resistance to or synergy with trametinib, a Ras/ERK/ETS inhibitor, or rapamycin, an mTOR inhibitor, and identified key regulators of each pathway. The results present a novel, scalable, and versatile platform for functional genomic screens in low-redundancy animal cells." Interested to design a CRISPR pooled drop-out or selection screen to interrogate a cellular function you are interested in? Please contact the Director S. Mohr. We are actively seeking collaborations on this exciting new approach.