CRISPR gRNA design

Content tagged with CRISPR gRNA design

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Entomological Society of America "Entomology 2024" meeting

Event
We've submitted abstracts and plan to participate in the Entomological Society of America's Entomology 2024 conference in Phoneix, AZ, this fall to connect with new audiences as we continue development of our CRISPR screening platform for screens in...

DRSC/TRiP and DRSC-BTRR Office Hours

News
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...

New publications from the DRSC bioinformatics team

News
The DRSC bioinformatics team, led by Dr. Claire Yanhui Hu, has recently published two new papers. One reports development of BioLitMine, an advanced literature mining resource. The other provides an overview of our online resources, which can be grouped...

DRSC-BTRR at the GSA Molecular Parasitology Meeting XXXI

Event
The Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR) will be represented at the GSA's Molecular Parasitology Meeting XXXI (a virtual event). Look for a presentation by Viswanatha et al. on our work establishing...

DRSC-Biomedical Technology Research Resource

News
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...

DRSC & TRiP Workshop at ADRC

Event
The DRSC & TRiP will be hosting a workshop at the Annual Drosophila Research Conference in Philadelphia, PA. The workshop is scheduled for Friday, April 13th from 1:45 to 3:45 PM. Come hear from DRSC & TRiP leaders Norbert Perrimon, Jonathan Zirin...

The TRiP is now taking nominations for CRISPR stocks

News
The TRiP is excited to announce our new TRiP-CRISPR transgenic fly libraries for gene knockout and overexpression. Visit the sgRNA tracking site to download and search existing TRiP-CRISPR fly stocks by gene or stock ID to obtain information on sgRNA...

Bibliographic References tagged with CRISPR gRNA design

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Chiao-Lin Chen, Jonathan Rodiger, Verena Chung, Raghuvir Viswanatha, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2019. “SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing”. G3 (Bethesda). doi:10.1534/g3.119.400904
Chiao-Lin Chen, Jonathan Rodiger, Verena Chung, Raghuvir Viswanatha, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2019. “SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing”. G3 (Bethesda). doi:10.1534/g3.119.400904
Raghuvir Viswanatha, Roderick Brathwaite, Yanhui Hu, Zhongchi Li, Jonathan Rodiger, Pierre Merckaert, Verena Chung, Stephanie Mohr, and Norbert Perrimon. 2019. “Pooled CRISPR Screens in Drosophila Cells”. Curr Protoc Mol Biol, 129, 1, Pp. e111. doi:10.1002/cpmb.111
Raghuvir Viswanatha, Roderick Brathwaite, Yanhui Hu, Zhongchi Li, Jonathan Rodiger, Pierre Merckaert, Verena Chung, Stephanie Mohr, and Norbert Perrimon. 2019. “Pooled CRISPR Screens in Drosophila Cells”. Curr Protoc Mol Biol, 129, 1, Pp. e111. doi:10.1002/cpmb.111
Oguz Kanca, Jonathan Zirin, Jorge Garcia-Marques, Shannon Marie Knight, Donghui Yang-Zhou, Gabriel Amador, Hyunglok Chung, Zhongyuan Zuo, Liwen Ma, Yuchun He, Wen-Wen Lin, Ying Fang, Ming Ge, Shinya Yamamoto, Karen Schulze, Yanhui Hu, Allan Spradling, Stephanie Mohr, Norbert Perrimon, and Hugo Bellen. 2019. “An Efficient CRISPR-Based Strategy to Insert Small and Large Fragments of DNA Using Short Homology Arms”. Elife, 8. doi:10.7554/eLife.51539
Oguz Kanca, Jonathan Zirin, Jorge Garcia-Marques, Shannon Marie Knight, Donghui Yang-Zhou, Gabriel Amador, Hyunglok Chung, Zhongyuan Zuo, Liwen Ma, Yuchun He, Wen-Wen Lin, Ying Fang, Ming Ge, Shinya Yamamoto, Karen Schulze, Yanhui Hu, Allan Spradling, Stephanie Mohr, Norbert Perrimon, and Hugo Bellen. 2019. “An Efficient CRISPR-Based Strategy to Insert Small and Large Fragments of DNA Using Short Homology Arms”. Elife, 8. doi:10.7554/eLife.51539
Yanhui Hu, Aram Comjean, Charles Roesel, Arunachalam Vinayagam, Ian Flockhart, Jonathan Zirin, Lizabeth Perkins, Norbert Perrimon, and Stephanie Mohr. 2016. “FlyRNAi.Org—the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2017 Update”. Nucleic Acids Research
Yanhui Hu, Aram Comjean, Charles Roesel, Arunachalam Vinayagam, Ian Flockhart, Jonathan Zirin, Lizabeth Perkins, Norbert Perrimon, and Stephanie Mohr. 2016. “FlyRNAi.Org—the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2017 Update”. Nucleic Acids Research
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet
Benjamin Housden, Shuailiang Lin, and Norbert Perrimon. 2014. “Cas9-Based Genome Editing in Drosophila.”. Methods Enzymol, 546, Pp. 415-39. doi:10.1016/B978-0-12-801185-0.00019-2
Benjamin Housden, Shuailiang Lin, and Norbert Perrimon. 2014. “Cas9-Based Genome Editing in Drosophila.”. Methods Enzymol, 546, Pp. 415-39. doi:10.1016/B978-0-12-801185-0.00019-2
Stephanie Mohr, Yanhui Hu, Benjamin Ewen-Campen, Benjamin Housden, Raghuvir Viswanatha, and Norbert Perrimon. 2016. “CRISPR Guide RNA Design for Research Applications.”. FEBS J. doi:10.1111/febs.13777
Stephanie Mohr, Yanhui Hu, Benjamin Ewen-Campen, Benjamin Housden, Raghuvir Viswanatha, and Norbert Perrimon. 2016. “CRISPR Guide RNA Design for Research Applications.”. FEBS J. doi:10.1111/febs.13777
Xingjie Ren, Jin Sun, Benjamin Housden, Yanhui Hu, Charles Roesel, Shuailiang Lin, Lu-Ping Liu, Zhihao Yang, Decai Mao, Lingzhu Sun, Qujie Wu, Jun-Yuan Ji, Jianzhong Xi, Stephanie Mohr, Jiang Xu, Norbert Perrimon, and Jian-Quan Ni. 2013. “Optimized Gene Editing Technology for Drosophila Melanogaster Using Germ Line-Specific Cas9.”. Proc Natl Acad Sci U S A, 110, 47, Pp. 19012-7. doi:10.1073/pnas.1318481110
Xingjie Ren, Jin Sun, Benjamin Housden, Yanhui Hu, Charles Roesel, Shuailiang Lin, Lu-Ping Liu, Zhihao Yang, Decai Mao, Lingzhu Sun, Qujie Wu, Jun-Yuan Ji, Jianzhong Xi, Stephanie Mohr, Jiang Xu, Norbert Perrimon, and Jian-Quan Ni. 2013. “Optimized Gene Editing Technology for Drosophila Melanogaster Using Germ Line-Specific Cas9.”. Proc Natl Acad Sci U S A, 110, 47, Pp. 19012-7. doi:10.1073/pnas.1318481110