RNAi reagent design

Content tagged with RNAi reagent design

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Important new considerations for TRiP stock users

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A handful of recent papers and preprints have highlighted some important considerations for using TRiP shRNA and sgRNA stocks. First, the Weake lab at Purdue University discovered that TRiP stocks contain a previously uncharacterized loss-of-function...

DRSC/TRiP and DRSC-BTRR Office Hours

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New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...

New publications from the DRSC bioinformatics team

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The DRSC bioinformatics team, led by Dr. Claire Yanhui Hu, has recently published two new papers. One reports development of BioLitMine, an advanced literature mining resource. The other provides an overview of our online resources, which can be grouped...

Micropublication relevant to TRiP fly stocks

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Users of TRiP RNAi and sgRNA fly stocks take note: the Weake lab at Purdue University brought to our attention that some TRiP fly stocks carry a mutant allele of seveneless. Jonathan Zirin worked with Spencer Escobedo and Vikki Weake, as well as with...

Now Available: Our 2017 NAR database issue update

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Read about the DRSC/TRiP Functional Genomics Resources online tools and more in our 2017 Nucleic Acids Research database issue update publication. Tables 1 & 2 in particular are meant to give you a quick look at what online tools we offer and how they fit...

Publication describes TRiP resources

News
Liz Perkins and colleagues have published a paper describing the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School. The article, published in the November 1, 2015 issue of Genetics, details the TRiP production pipeline , reagents...

Bibliographic References tagged with RNAi reagent design

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Jian-Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker, Lizabeth Perkins, and Norbert Perrimon. 2008. “Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila Melanogaster.”. Nat Methods, 5, 1, Pp. 49-51. doi:10.1038/nmeth1146
Jian-Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker, Lizabeth Perkins, and Norbert Perrimon. 2008. “Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila Melanogaster.”. Nat Methods, 5, 1, Pp. 49-51. doi:10.1038/nmeth1146
Stephanie Mohr, Chris Bakal, and Norbert Perrimon. 2010. “Genomic Screening With RNAi: Results and Challenges.”. Annu Rev Biochem, 79, Pp. 37-64. doi:10.1146/annurev-biochem-060408-092949
Stephanie Mohr, Chris Bakal, and Norbert Perrimon. 2010. “Genomic Screening With RNAi: Results and Challenges.”. Annu Rev Biochem, 79, Pp. 37-64. doi:10.1146/annurev-biochem-060408-092949
Norbert Perrimon, Jian-Quan Ni, and Lizabeth Perkins. 2010. “In Vivo RNAi: Today and Tomorrow.”. Cold Spring Harb Perspect Biol, 2, 8, Pp. a003640. doi:10.1101/cshperspect.a003640
Norbert Perrimon, Jian-Quan Ni, and Lizabeth Perkins. 2010. “In Vivo RNAi: Today and Tomorrow.”. Cold Spring Harb Perspect Biol, 2, 8, Pp. a003640. doi:10.1101/cshperspect.a003640
Matthew Booker, Anastasia Samsonova, Young Kwon, Ian Flockhart, Stephanie Mohr, and Norbert Perrimon. 2011. “False Negative Rates in Drosophila Cell-Based RNAi Screens: A Case Study.”. BMC Genomics, 12, Pp. 50. doi:10.1186/1471-2164-12-50
Matthew Booker, Anastasia Samsonova, Young Kwon, Ian Flockhart, Stephanie Mohr, and Norbert Perrimon. 2011. “False Negative Rates in Drosophila Cell-Based RNAi Screens: A Case Study.”. BMC Genomics, 12, Pp. 50. doi:10.1186/1471-2164-12-50
Stephanie Mohr and Norbert Perrimon. 2012. “RNAi Screening: New Approaches, Understandings, and Organisms.”. Wiley Interdiscip Rev RNA, 3, 2, Pp. 145-58. doi:10.1002/wrna.110
Stephanie Mohr and Norbert Perrimon. 2012. “RNAi Screening: New Approaches, Understandings, and Organisms.”. Wiley Interdiscip Rev RNA, 3, 2, Pp. 145-58. doi:10.1002/wrna.110
Max Staller, Dong Yan, Sakara Randklev, Meghan Bragdon, Zeba Wunderlich, Rong Tao, Lizabeth Perkins, Angela Depace, and Norbert Perrimon. 2013. “Depleting Gene Activities in Early Drosophila Embryos With the ‘maternal-Gal4-ShRNA’ System.”. Genetics, 193, 1, Pp. 51-61. doi:10.1534/genetics.112.144915
Max Staller, Dong Yan, Sakara Randklev, Meghan Bragdon, Zeba Wunderlich, Rong Tao, Lizabeth Perkins, Angela Depace, and Norbert Perrimon. 2013. “Depleting Gene Activities in Early Drosophila Embryos With the ‘maternal-Gal4-ShRNA’ System.”. Genetics, 193, 1, Pp. 51-61. doi:10.1534/genetics.112.144915
Yanhui Hu, Charles Roesel, Ian Flockhart, Lizabeth Perkins, Norbert Perrimon, and Stephanie Mohr. 2013. “UP-TORR: Online Tool for Accurate and Up-to-Date Annotation of RNAi Reagents.”. Genetics, 195, 1, Pp. 37-45. doi:10.1534/genetics.113.151340
Yanhui Hu, Charles Roesel, Ian Flockhart, Lizabeth Perkins, Norbert Perrimon, and Stephanie Mohr. 2013. “UP-TORR: Online Tool for Accurate and Up-to-Date Annotation of RNAi Reagents.”. Genetics, 195, 1, Pp. 37-45. doi:10.1534/genetics.113.151340
Stephanie Mohr, Yanhui Hu, Kevin Kim, Benjamin Housden, and Norbert Perrimon. 2014. “Resources for Functional Genomics Studies in Drosophila Melanogaster.”. Genetics, 197, 1, Pp. 1-18. doi:10.1534/genetics.113.154344
Stephanie Mohr, Yanhui Hu, Kevin Kim, Benjamin Housden, and Norbert Perrimon. 2014. “Resources for Functional Genomics Studies in Drosophila Melanogaster.”. Genetics, 197, 1, Pp. 1-18. doi:10.1534/genetics.113.154344