Cell-based assays
Content tagged with Cell-based assays
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Event
We've submitted abstracts and plan to participate in the Entomological Society of America's Entomology 2024 conference in Phoneix, AZ, this fall to connect with new audiences as we continue development of our CRISPR screening platform for screens in...
New cell lines & new understandings using cutting-edge techniques
News
As a facility that supports large-scale screens in Drosophila and other insect cell lines, we get excited about reports of new Drosophila cell lines and related info. We'd like to highlight two recent papers. One report, a collaboration between Amanda...
Virtual CRISPR Workshop 'at' ADRC 2023
Event
The DRSC is collaborating with other groups to present a virtual workshop on CRISPR technologies for Drosophila cells and in vivo as part of the GSA Annual Drosophila Research Conference 2023.
DRSC & DRTC Genetics and Genomics Workshop
Event
The DRSC is collaborating with the Drosophila Research and Training Center of Nigeria and the laboratory of Prof. Ross Cagan (University of Glasgow) to present an online workshop on Drosophila genomics and genetics.
So you want to do a CRISPR pooled screen in insect cells? You can! Here's how
News
At the DRSC-BTRR, we've been doing a lot of pooled-format CRISPR knockout screens in Drosophila cells. We're finding the results to be robust and reproducible. And best of all, the results have been informative, providing insights into diverse areas of...
Read the DRSC/TRiP Report to the Fly Board 2022
News
Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help. DRSC/TRiP Functional Genomics Resources at...
DRSC/TRiP and DRSC-BTRR Office Hours
News
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...
DRSC-Biomedical Technology Research Resource
News
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...
Drosophila cell screen with DRSC reagent library contributes to identification of new therapeutic target for renal cancer
News
We here at the DRSC/TRiP are thrilled to see this study from Hilary Nicholson et al. published in Science Signaling. The study provides a great example of how screens in Drosophila cultured cells can be used as part of a cross-species platform aimed at...
DRSC&TRiP at ASCB|EMBO 2018
Event
The DRSC & TRiP will be represented at the ASCB|EMBO 2018 conference in the form of a poster that includes information about our collaboration with O. Kanca in the H. Bellen lab at Baylor College of Medicine to tag Drosophila S2R+ cell lines with GFP...
Bibliographic References tagged with Cell-based assays
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Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595
Enzo Mameli, George-Rafael Samantsidis, Raghuvir Viswanatha, Hyeogsun Kwon, David R Hall, Matthew Butnaru, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon, and Ryan C Smith. 2024. “A Genome-Wide CRISPR Screen in Mosquito Cells Identifies Essential Genes and Required Components of Clodronate Liposome Function.”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.24.614595
Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness”. BioRxiv, Pp. 2023.08.31.555785
Agustin Rolandelli, Hanna Laukaitis-Yousey, Haikel Bogale, Nisha Singh, Sourabh Samaddar, Anya O’Neal, Camila Ferraz, Matthew Butnaru, Enzo Mameli, Baolong Xia, Tays Mendes, Rainer Butler, Liron Marnin, Francy Paz, Luisa Valencia, Vipin Rana, Ciaran Skerry, Utpal Pal, Stephanie Mohr, Norbert Perrimon, David Serre, and Joao Pedra. 2023. “Tick Hemocytes Have Pleiotropic Roles in Microbial Infection and Arthropod Fitness”. BioRxiv, Pp. 2023.08.31.555785
Nikki Coleman-Gosser, Yanhui Hu, Shiva Raghuvanshi, Shane Stitzinger, Weihang Chen, Arthur Luhur, Daniel Mariyappa, Molly Josifov, Andrew Zelhof, Stephanie Mohr, Norbert Perrimon, and Amanda Simcox. 2023. “Continuous Muscle, Glial, Epithelial, Neuronal, and Hemocyte Cell Lines for Research”. Elife, 12. doi:10.7554/eLife.85814
Nikki Coleman-Gosser, Yanhui Hu, Shiva Raghuvanshi, Shane Stitzinger, Weihang Chen, Arthur Luhur, Daniel Mariyappa, Molly Josifov, Andrew Zelhof, Stephanie Mohr, Norbert Perrimon, and Amanda Simcox. 2023. “Continuous Muscle, Glial, Epithelial, Neuronal, and Hemocyte Cell Lines for Research”. Elife, 12. doi:10.7554/eLife.85814
Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells”. Elife, 12. doi:10.7554/eLife.85542
Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie Mohr, and Norbert Perrimon. 2023. “Pooled Genome-Wide CRISPR Activation Screening for Rapamycin Resistance Genes in Cells”. Elife, 12. doi:10.7554/eLife.85542
Shue Chen, Leah Rosin, Gianluca Pegoraro, Nellie Moshkovich, Patrick Murphy, Guoyun Yu, and Elissa Lei. 2022. “NURF301 Contributes to Gypsy Chromatin Insulator-Mediated Nuclear Organization”. Nucleic Acids Res, 50, 14, Pp. 7906-24. doi:10.1093/nar/gkac600
Shue Chen, Leah Rosin, Gianluca Pegoraro, Nellie Moshkovich, Patrick Murphy, Guoyun Yu, and Elissa Lei. 2022. “NURF301 Contributes to Gypsy Chromatin Insulator-Mediated Nuclear Organization”. Nucleic Acids Res, 50, 14, Pp. 7906-24. doi:10.1093/nar/gkac600
Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7
Ying Xu, Raghuvir Viswanatha, Oleg Sitsel, Daniel Roderer, Haifang Zhao, Christopher Ashwood, Cecilia Voelcker, Songhai Tian, Stefan Raunser, Norbert Perrimon, and Min Dong. 2022. “CRISPR Screens in Drosophila Cells Identify Vsg As a Tc Toxin Receptor”. Nature, 610, 7931, Pp. 349-55. doi:10.1038/s41586-022-05250-7
Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430
Hans Dalton, Raghuvir Viswanatha, Roderick Brathwaite, Jae Sophia Zuno, Alexys Berman, Rebekah Rushforth, Stephanie Mohr, Norbert Perrimon, and Clement Chow. 2022. “A Genome-Wide CRISPR Screen Identifies DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. PLoS Genet, 18, 9, Pp. e1010430. doi:10.1371/journal.pgen.1010430
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Wei-Chun Tang, Chieh-Han Lu, Stephanie E. Mohr, Robert V. Farese, and Tobias C. Walther. 2022. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets”. Nature Cell Biol
Jiunn Song, Arda Mizrak, Chia-Wei Lee, Marcelo Cicconet, Zon Weng Lai, Wei-Chun Tang, Chieh-Han Lu, Stephanie E. Mohr, Robert V. Farese, and Tobias C. Walther. 2022. “Identification of Two Pathways Mediating Protein Targeting from ER to Lipid Droplets”. Nature Cell Biol
Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. BioRxiv
Hans M. Dalton, Raghuvir Viswanatha, Ricky Brathwaite Jr., Jae Sophia Zuno, Stephanie Mohr, Norbert Perrimon, and Clement Y. Chow. 2021. “A Genome-Wide CRISPR Screen Identifies the Glycosylation Enzyme DPM1 As a Modifier of DPAGT1 Deficiency and ER Stress”. BioRxiv
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3