Search

CRISPR guide RNA design for research applications.

Journal Article

The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on-target and effective guide RNAs (gRNAs). Here, we review the state...

Find CRISPRs

Page

This tool provides access to a pre-computed database of short guide RNAs (gRNAs) for CRISPR approaches in Drosophila. The gRNAs are appropriate for CRISPR applications using S. pyogenes Cas9 (PAM sequence of NGG or NAG). You can view and filter gRNAs in...

CRISPR Efficiency Prediction

Page

Part of our "Find CRISPRs" design suite, our Efficiency Predictor evaluation tool compares the nucleotide sequence of short guide RNA (gRNA) designs against our algorithms for optimal efficiency. Note that this tool does not evaluate potential off targets...

Cell-based CRISPR

Page

We published a detailed protocol describing two methods and comparing among three methods for CRISPR knock-in in Drosophila. We also have expertise in knockout and have deposited both knock-in and knock-out cell lines at the Drosophila Genomics Resource...

CRISPR Efficiency Prediction Documentation

Page

URL http://www.flyrnai.org/evaluateCrispr/ CRISPR Efficiency Prediction Tool Help Page The tool reports a predicted efficiency score based on nucleotide sequence. The score reflects cumulative p-value for high efficiency based on in vitro data generated...

CRISPR sgRNA design tool now based on Drosophila genome assembly 6

News

We have updated our CRISPR sgRNA design tool. The results and JBrowse display are now based on Drosophila melanogaster FlyBase genome assembly release 6. We have also added seed scores in addition to efficiency prediction scores and other values available...

in vivo CRISPR (TRiP)

Page

Quick link to Find CRISPR sgRNA search Quick link to TRiP-CRISPR sgRNA stock database Using the TRiP pipeline, we are actively developing CRISPR fly stocks and resources for gene editing and other applications. These resources facilitate knockout, up...

SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing

Journal Article

CRISPR-Cas9 is a powerful genome editing technology in which a short guide RNA (sgRNA) confers target site specificity to achieve Cas9-mediated genome editing. Numerous sgRNA design tools have been developed based on reference genomes for humans and model...

Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Journal Article

Mosquito-borne diseases present a worldwide public health burden. Current efforts to understand and counteract them have been aided by the use of cultured mosquito cells. Moreover, application in mammalian cells of forward genetic approaches such as...

An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

Journal Article

We previously reported a CRISPR-mediated knock-in strategy into introns of genes, generating an - transgenic library for multiple uses (Lee et al., 2018b). The method relied on double stranded DNA (dsDNA) homology donors with ~1 kb homology arms. Here, we...

The TRiP is now taking nominations for CRISPR stocks

News

The TRiP is excited to announce our new TRiP-CRISPR transgenic fly libraries for gene knockout and overexpression. Visit the sgRNA tracking site to download and search existing TRiP-CRISPR fly stocks by gene or stock ID to obtain information on sgRNA...

Pooled CRISPR Screens in Drosophila Cells

Journal Article

High-throughput screens in Drosophila melanogaster cell lines have led to discovery of conserved gene functions related to signal transduction, host-pathogen interactions, ion transport, and more. CRISPR/Cas9 technology has opened the door to new types of...