in vivo fly CRISPR

Content tagged with in vivo fly CRISPR

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Virtual CRISPR Workshop 'at' ADRC 2023

Event
The DRSC is collaborating with other groups to present a virtual workshop on CRISPR technologies for Drosophila cells and in vivo as part of the GSA Annual Drosophila Research Conference 2023.

DRSC & DRTC Genetics and Genomics Workshop

Event
The DRSC is collaborating with the Drosophila Research and Training Center of Nigeria and the laboratory of Prof. Ross Cagan (University of Glasgow) to present an online workshop on Drosophila genomics and genetics.

Important new considerations for TRiP stock users

News
A handful of recent papers and preprints have highlighted some important considerations for using TRiP shRNA and sgRNA stocks. First, the Weake lab at Purdue University discovered that TRiP stocks contain a previously uncharacterized loss-of-function...

Read the DRSC/TRiP Report to the Fly Board 2022

News
Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help. DRSC/TRiP Functional Genomics Resources at...

DRSC/TRiP and DRSC-BTRR Office Hours

News
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...

Transgenic Fly Stocks for Double Knockout of Paralog Pairs

News
Paralogs can be defined as related genes within a genome that are thought to arise from gene duplication events. Because paralogous proteins share amino acid identity, they can have redundant functions. But the picture is not necessarily so...

DRSC-Biomedical Technology Research Resource

News
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...

Micropublication relevant to TRiP fly stocks

News
Users of TRiP RNAi and sgRNA fly stocks take note: the Weake lab at Purdue University brought to our attention that some TRiP fly stocks carry a mutant allele of seveneless. Jonathan Zirin worked with Spencer Escobedo and Vikki Weake, as well as with...

Director S. Mohr presents at the Countway Longwood Author Series

Event
On June 18th, DRSC/TRiP-FGR Director Dr. Stephanie Mohr will present an introduction to Drosophila research and discuss her book First in Fly: Drosophila Research and Biological Discussion, as part of the Countway Longwood Author Series. The event will be...

Bibliographic References tagged with in vivo fly CRISPR

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Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
Justin A. Bosch, Norbert Perrimon, and Christian Dahmann. 2022. “Prime Editing for Precise Genome Engineering in Drosophila”. In Drosophila: Methods and Protocols, Pp. 113-34. New York, NY: Springer US
Justin A. Bosch, Norbert Perrimon, and Christian Dahmann. 2022. “Prime Editing for Precise Genome Engineering in Drosophila”. In Drosophila: Methods and Protocols, Pp. 113-34. New York, NY: Springer US
Jun Xu, Ah-Ram Kim, Ross W. Cheloha, Fabian A. Fischer, Joshua Shing Shun Li, Yuan Feng, Emily Stoneburner, Richard Binari, Stephanie E. Mohr, Jonathan Zirin, Hidde Ploegh, and Norbert Perrimon. 2021. “Protein Visualization and Manipulation in Drosophila through the Use of Epitope Tags Recognized by Nanobodies”. BioRxiv
Jun Xu, Ah-Ram Kim, Ross W. Cheloha, Fabian A. Fischer, Joshua Shing Shun Li, Yuan Feng, Emily Stoneburner, Richard Binari, Stephanie E. Mohr, Jonathan Zirin, Hidde Ploegh, and Norbert Perrimon. 2021. “Protein Visualization and Manipulation in Drosophila through the Use of Epitope Tags Recognized by Nanobodies”. BioRxiv
R. Viswanatha, M. Zaffagni, J. Zirin, N. Perrimon, and S. Kadener. 2020. “CRISPR-Cas13 Mediated Knock Down in Drosophila Cultured Cells”. BioRxiv
R. Viswanatha, M. Zaffagni, J. Zirin, N. Perrimon, and S. Kadener. 2020. “CRISPR-Cas13 Mediated Knock Down in Drosophila Cultured Cells”. BioRxiv
J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “Precise Genome Engineering in Drosophila Using Prime Editing”. Proc Natl Acad Sci U S A, 118
J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “Precise Genome Engineering in Drosophila Using Prime Editing”. Proc Natl Acad Sci U S A, 118
Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies”. Genetics. doi:10.1534/genetics.119.302964
Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies”. Genetics. doi:10.1534/genetics.119.302964
Oguz Kanca, Jonathan Zirin, Jorge Garcia-Marques, Shannon Marie Knight, Donghui Yang-Zhou, Gabriel Amador, Hyunglok Chung, Zhongyuan Zuo, Liwen Ma, Yuchun He, Wen-Wen Lin, Ying Fang, Ming Ge, Shinya Yamamoto, Karen Schulze, Yanhui Hu, Allan Spradling, Stephanie Mohr, Norbert Perrimon, and Hugo Bellen. 2019. “An Efficient CRISPR-Based Strategy to Insert Small and Large Fragments of DNA Using Short Homology Arms”. Elife, 8. doi:10.7554/eLife.51539
Oguz Kanca, Jonathan Zirin, Jorge Garcia-Marques, Shannon Marie Knight, Donghui Yang-Zhou, Gabriel Amador, Hyunglok Chung, Zhongyuan Zuo, Liwen Ma, Yuchun He, Wen-Wen Lin, Ying Fang, Ming Ge, Shinya Yamamoto, Karen Schulze, Yanhui Hu, Allan Spradling, Stephanie Mohr, Norbert Perrimon, and Hugo Bellen. 2019. “An Efficient CRISPR-Based Strategy to Insert Small and Large Fragments of DNA Using Short Homology Arms”. Elife, 8. doi:10.7554/eLife.51539
Spencer E. Escobedo, Jonathan Zirin, and Vikki M. Weake. 2019. “TRiP Stocks Contain a Previously Uncharacterized Loss-of-Function Sevenless Allele”. MicroPublication Biology
Spencer E. Escobedo, Jonathan Zirin, and Vikki M. Weake. 2019. “TRiP Stocks Contain a Previously Uncharacterized Loss-of-Function Sevenless Allele”. MicroPublication Biology
Ben Ewen-Campen, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2017. “Accessing the Phenotype Gap: Enabling Systematic Investigation of Paralog Functional Complexity With CRISPR”. Dev Cell, 43, 1, Pp. 6-9. doi:10.1016/j.devcel.2017.09.020
Ben Ewen-Campen, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2017. “Accessing the Phenotype Gap: Enabling Systematic Investigation of Paralog Functional Complexity With CRISPR”. Dev Cell, 43, 1, Pp. 6-9. doi:10.1016/j.devcel.2017.09.020
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet