CRISPR gRNA design

Content tagged with CRISPR gRNA design

Not finding what you're looking for? Try using Advanced Search.
Not finding what you're looking for? Try using Advanced Search.

Virtual CRISPR Workshop 'at' ADRC 2023

Event
The DRSC is collaborating with other groups to present a virtual workshop on CRISPR technologies for Drosophila cells and in vivo as part of the GSA Annual Drosophila Research Conference 2023.

DRSC/TRiP and DRSC-BTRR Office Hours

News
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...

DRSC-Biomedical Technology Research Resource

News
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...

Pooled-format CRISPR screens in Drosophila cells

News
The DRSC/TRiP-FGR is pleased to support collaborations on pooled CRISPR screens using the method recently, reported in eLife by Viswanatha et al. (PDF download file below). From the abstract: "... Here, we developed a site-specific integration strategy...

DRSC & TRiP Workshop at ADRC

Event
The DRSC & TRiP will be hosting a workshop at the Annual Drosophila Research Conference in Philadelphia, PA. The workshop is scheduled for Friday, April 13th from 1:45 to 3:45 PM. Come hear from DRSC & TRiP leaders Norbert Perrimon, Jonathan Zirin...

The TRiP is now taking nominations for CRISPR stocks

News
The TRiP is excited to announce our new TRiP-CRISPR transgenic fly libraries for gene knockout and overexpression. Visit the sgRNA tracking site to download and search existing TRiP-CRISPR fly stocks by gene or stock ID to obtain information on sgRNA...

Presentation at Genome Editing and Trangenic Congress USA

Event
Stephanie will be presenting at the Genome Editing and Transgenic Congress USA in Boston, MA, on May 10, 2017 (time TBD). She plans to cover the following topics. Strategy and results of optimization of Cas9 fusion proteins for CRISPR activation in cells...

Now Available: Our 2017 NAR database issue update

News
Read about the DRSC/TRiP Functional Genomics Resources online tools and more in our 2017 Nucleic Acids Research database issue update publication. Tables 1 & 2 in particular are meant to give you a quick look at what online tools we offer and how they fit...

Bibliographic References tagged with CRISPR gRNA design

Not finding what you're looking for? Try using Advanced Search.
Not finding what you're looking for? Try using Advanced Search.
Raghuvir Viswanatha, Roderick Brathwaite, Yanhui Hu, Zhongchi Li, Jonathan Rodiger, Pierre Merckaert, Verena Chung, Stephanie Mohr, and Norbert Perrimon. 2019. “Pooled CRISPR Screens in Drosophila Cells”. Curr Protoc Mol Biol, 129, 1, Pp. e111. doi:10.1002/cpmb.111
Raghuvir Viswanatha, Roderick Brathwaite, Yanhui Hu, Zhongchi Li, Jonathan Rodiger, Pierre Merckaert, Verena Chung, Stephanie Mohr, and Norbert Perrimon. 2019. “Pooled CRISPR Screens in Drosophila Cells”. Curr Protoc Mol Biol, 129, 1, Pp. e111. doi:10.1002/cpmb.111
Oguz Kanca, Jonathan Zirin, Jorge Garcia-Marques, Shannon Marie Knight, Donghui Yang-Zhou, Gabriel Amador, Hyunglok Chung, Zhongyuan Zuo, Liwen Ma, Yuchun He, Wen-Wen Lin, Ying Fang, Ming Ge, Shinya Yamamoto, Karen Schulze, Yanhui Hu, Allan Spradling, Stephanie Mohr, Norbert Perrimon, and Hugo Bellen. 2019. “An Efficient CRISPR-Based Strategy to Insert Small and Large Fragments of DNA Using Short Homology Arms”. Elife, 8. doi:10.7554/eLife.51539
Oguz Kanca, Jonathan Zirin, Jorge Garcia-Marques, Shannon Marie Knight, Donghui Yang-Zhou, Gabriel Amador, Hyunglok Chung, Zhongyuan Zuo, Liwen Ma, Yuchun He, Wen-Wen Lin, Ying Fang, Ming Ge, Shinya Yamamoto, Karen Schulze, Yanhui Hu, Allan Spradling, Stephanie Mohr, Norbert Perrimon, and Hugo Bellen. 2019. “An Efficient CRISPR-Based Strategy to Insert Small and Large Fragments of DNA Using Short Homology Arms”. Elife, 8. doi:10.7554/eLife.51539
Yanhui Hu, Aram Comjean, Charles Roesel, Arunachalam Vinayagam, Ian Flockhart, Jonathan Zirin, Lizabeth Perkins, Norbert Perrimon, and Stephanie Mohr. 2016. “FlyRNAi.Org—the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2017 Update”. Nucleic Acids Research
Yanhui Hu, Aram Comjean, Charles Roesel, Arunachalam Vinayagam, Ian Flockhart, Jonathan Zirin, Lizabeth Perkins, Norbert Perrimon, and Stephanie Mohr. 2016. “FlyRNAi.Org—the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2017 Update”. Nucleic Acids Research
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet
Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences”. Nat Rev Genet
Benjamin Housden, Shuailiang Lin, and Norbert Perrimon. 2014. “Cas9-Based Genome Editing in Drosophila.”. Methods Enzymol, 546, Pp. 415-39. doi:10.1016/B978-0-12-801185-0.00019-2
Benjamin Housden, Shuailiang Lin, and Norbert Perrimon. 2014. “Cas9-Based Genome Editing in Drosophila.”. Methods Enzymol, 546, Pp. 415-39. doi:10.1016/B978-0-12-801185-0.00019-2
Stephanie Mohr, Yanhui Hu, Benjamin Ewen-Campen, Benjamin Housden, Raghuvir Viswanatha, and Norbert Perrimon. 2016. “CRISPR Guide RNA Design for Research Applications.”. FEBS J. doi:10.1111/febs.13777
Stephanie Mohr, Yanhui Hu, Benjamin Ewen-Campen, Benjamin Housden, Raghuvir Viswanatha, and Norbert Perrimon. 2016. “CRISPR Guide RNA Design for Research Applications.”. FEBS J. doi:10.1111/febs.13777
Xingjie Ren, Jin Sun, Benjamin Housden, Yanhui Hu, Charles Roesel, Shuailiang Lin, Lu-Ping Liu, Zhihao Yang, Decai Mao, Lingzhu Sun, Qujie Wu, Jun-Yuan Ji, Jianzhong Xi, Stephanie Mohr, Jiang Xu, Norbert Perrimon, and Jian-Quan Ni. 2013. “Optimized Gene Editing Technology for Drosophila Melanogaster Using Germ Line-Specific Cas9.”. Proc Natl Acad Sci U S A, 110, 47, Pp. 19012-7. doi:10.1073/pnas.1318481110
Xingjie Ren, Jin Sun, Benjamin Housden, Yanhui Hu, Charles Roesel, Shuailiang Lin, Lu-Ping Liu, Zhihao Yang, Decai Mao, Lingzhu Sun, Qujie Wu, Jun-Yuan Ji, Jianzhong Xi, Stephanie Mohr, Jiang Xu, Norbert Perrimon, and Jian-Quan Ni. 2013. “Optimized Gene Editing Technology for Drosophila Melanogaster Using Germ Line-Specific Cas9.”. Proc Natl Acad Sci U S A, 110, 47, Pp. 19012-7. doi:10.1073/pnas.1318481110