CRISPR gRNA design

Content tagged with CRISPR gRNA design

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Virtual CRISPR Workshop 'at' ADRC 2023

Event
The DRSC is collaborating with other groups to present a virtual workshop on CRISPR technologies for Drosophila cells and in vivo as part of the GSA Annual Drosophila Research Conference 2023.

DRSC/TRiP and DRSC-BTRR Office Hours

News
New this fall: Online office hours! Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly...

DRSC-Biomedical Technology Research Resource

News
We are pleased to announce that we have been funded by NIH NIGMS to form the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR). The P41-funded DRSC-BTRR (N. Perrimon, PI; S. Mohr, Co-I) builds upon and extends past...

Pooled-format CRISPR screens in Drosophila cells

News
The DRSC/TRiP-FGR is pleased to support collaborations on pooled CRISPR screens using the method recently, reported in eLife by Viswanatha et al. (PDF download file below). From the abstract: "... Here, we developed a site-specific integration strategy...

DRSC & TRiP Workshop at ADRC

Event
The DRSC & TRiP will be hosting a workshop at the Annual Drosophila Research Conference in Philadelphia, PA. The workshop is scheduled for Friday, April 13th from 1:45 to 3:45 PM. Come hear from DRSC & TRiP leaders Norbert Perrimon, Jonathan Zirin...

The TRiP is now taking nominations for CRISPR stocks

News
The TRiP is excited to announce our new TRiP-CRISPR transgenic fly libraries for gene knockout and overexpression. Visit the sgRNA tracking site to download and search existing TRiP-CRISPR fly stocks by gene or stock ID to obtain information on sgRNA...

Presentation at Genome Editing and Trangenic Congress USA

Event
Stephanie will be presenting at the Genome Editing and Transgenic Congress USA in Boston, MA, on May 10, 2017 (time TBD). She plans to cover the following topics. Strategy and results of optimization of Cas9 fusion proteins for CRISPR activation in cells...

Now Available: Our 2017 NAR database issue update

News
Read about the DRSC/TRiP Functional Genomics Resources online tools and more in our 2017 Nucleic Acids Research database issue update publication. Tables 1 & 2 in particular are meant to give you a quick look at what online tools we offer and how they fit...

Bibliographic References tagged with CRISPR gRNA design

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Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos [NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.]”. BioRxiv. doi:10.1101/2021.03.29.437496
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos [NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.]”. BioRxiv. doi:10.1101/2021.03.29.437496
R. Viswanatha, M. Zaffagni, J. Zirin, N. Perrimon, and S. Kadener. 2020. “CRISPR-Cas13 Mediated Knock Down in Drosophila Cultured Cells”. BioRxiv
R. Viswanatha, M. Zaffagni, J. Zirin, N. Perrimon, and S. Kadener. 2020. “CRISPR-Cas13 Mediated Knock Down in Drosophila Cultured Cells”. BioRxiv
J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “Precise Genome Engineering in Drosophila Using Prime Editing”. Proc Natl Acad Sci U S A, 118
J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “Precise Genome Engineering in Drosophila Using Prime Editing”. Proc Natl Acad Sci U S A, 118
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies”. Genetics. doi:10.1534/genetics.119.302964
Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies”. Genetics. doi:10.1534/genetics.119.302964
Justin Bosch, Shannon Knight, Oguz Kanca, Jonathan Zirin, Donghui Yang-Zhou, Yanhui Hu, Jonathan Rodiger, Gabriel Amador, Hugo Bellen, Norbert Perrimon, and Stephanie Mohr. 2020. “Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes”. Curr Protoc Mol Biol, 130, 1, Pp. e112. doi:10.1002/cpmb.112
Justin Bosch, Shannon Knight, Oguz Kanca, Jonathan Zirin, Donghui Yang-Zhou, Yanhui Hu, Jonathan Rodiger, Gabriel Amador, Hugo Bellen, Norbert Perrimon, and Stephanie Mohr. 2020. “Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes”. Curr Protoc Mol Biol, 130, 1, Pp. e112. doi:10.1002/cpmb.112
Chiao-Lin Chen, Jonathan Rodiger, Verena Chung, Raghuvir Viswanatha, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2019. “SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing”. G3 (Bethesda). doi:10.1534/g3.119.400904
Chiao-Lin Chen, Jonathan Rodiger, Verena Chung, Raghuvir Viswanatha, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2019. “SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing”. G3 (Bethesda). doi:10.1534/g3.119.400904