CRISPR gRNA design

Content tagged with CRISPR gRNA design

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DRSC & TRiP at ADRC 2017

Event
The DRSC & TRiP will be at the Annual Drosophila Research Conference (aka 'the fly meeting') in San Diego, CA, in March/April of 2017. Please feel free to contact us if you'd like to set up a meeting to discuss a screen project or other research need we...

Beta-testing a "Drosophila Protocols Portal"

News
The DRSC-FGR has developed a beta version of a database and online search for protocols, the Drosophila Protocols Portal, relevant to Drosophila research. The goal is to provide a central portal for protocols distributed across the web. We collected...

Bibliographic References tagged with CRISPR gRNA design

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Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
Raghuvir Viswanatha, Samuel Entwisle, Claire Hu, Kelly Reap, Matthew Butnaru, Stephanie E Mohr, and Norbert Perrimon. 2024. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2024.09.19.613976
Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
Jonathan Zirin, Justin Bosch, Raghuvir Viswanatha, Stephanie Mohr, and Norbert Perrimon. 2022. “State-of-the-Art CRISPR for in Vivo and Cell-Based Studies in Drosophila”. Trends Genet, 38, 5, Pp. 437-53. doi:10.1016/j.tig.2021.11.006
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya Colpitts, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos”. Nat Commun, 12, 1, Pp. 6825. doi:10.1038/s41467-021-27129-3
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos [NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.]”. BioRxiv. doi:10.1101/2021.03.29.437496
Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, and Norbert Perrimon. 2021. “Bioinformatic and Cell-Based Tools for Pooled CRISPR Knockout Screening in Mosquitos [NOTE: A Modified Final Version Was Published in Nat Comm and Is Now Available.]”. BioRxiv. doi:10.1101/2021.03.29.437496
R. Viswanatha, M. Zaffagni, J. Zirin, N. Perrimon, and S. Kadener. 2020. “CRISPR-Cas13 Mediated Knock Down in Drosophila Cultured Cells”. BioRxiv
R. Viswanatha, M. Zaffagni, J. Zirin, N. Perrimon, and S. Kadener. 2020. “CRISPR-Cas13 Mediated Knock Down in Drosophila Cultured Cells”. BioRxiv
J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “Precise Genome Engineering in Drosophila Using Prime Editing”. Proc Natl Acad Sci U S A, 118
J. A. Bosch, G. Birchak, and N. Perrimon. 2021. “Precise Genome Engineering in Drosophila Using Prime Editing”. Proc Natl Acad Sci U S A, 118
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update”. Nucleic Acids Res. doi:10.1093/nar/gkaa936
Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies”. Genetics. doi:10.1534/genetics.119.302964
Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies”. Genetics. doi:10.1534/genetics.119.302964
Justin Bosch, Shannon Knight, Oguz Kanca, Jonathan Zirin, Donghui Yang-Zhou, Yanhui Hu, Jonathan Rodiger, Gabriel Amador, Hugo Bellen, Norbert Perrimon, and Stephanie Mohr. 2020. “Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes”. Curr Protoc Mol Biol, 130, 1, Pp. e112. doi:10.1002/cpmb.112
Justin Bosch, Shannon Knight, Oguz Kanca, Jonathan Zirin, Donghui Yang-Zhou, Yanhui Hu, Jonathan Rodiger, Gabriel Amador, Hugo Bellen, Norbert Perrimon, and Stephanie Mohr. 2020. “Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes”. Curr Protoc Mol Biol, 130, 1, Pp. e112. doi:10.1002/cpmb.112
Chiao-Lin Chen, Jonathan Rodiger, Verena Chung, Raghuvir Viswanatha, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2019. “SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing”. G3 (Bethesda). doi:10.1534/g3.119.400904
Chiao-Lin Chen, Jonathan Rodiger, Verena Chung, Raghuvir Viswanatha, Stephanie Mohr, Yanhui Hu, and Norbert Perrimon. 2019. “SNP-CRISPR: A Web Tool for SNP-Specific Genome Editing”. G3 (Bethesda). doi:10.1534/g3.119.400904