Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.
Publication information:
Jian-Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker, Lizabeth Perkins, and Norbert Perrimon. 2008. “Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila Melanogaster”. Nat Methods, 5, 1, Pp. 49-51. doi:10.1038/nmeth1146
Abstract
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.