Laboratories at the Skirball Institute at New York University and the Boyce Thompson Institute at Cornell University reported results of two different cell-based Drosophila RNAi screens in papers published this week. The screens have in common that they looked at interactions between the host insect cells and a microbe -- the endosymbiont Wolbachia in one study and baculovirus in the other. For more, check out the newly published studies. For both these screens, the DRSC provided libraries for screens that were then performed at the host institution.
Both continue our series of resources aimed at integrating existing information in new ways to facilitate data mining and development of new hypotheses. In addition, iProteinDB includes a new dataset related to post-transcriptional modification (in this case,...
At the DRSC/TRiP-Functional Genomics Resources, we are interested to let the community know about new resources we have built. We are also interested more broadly to help connect community members to additional resources that could help their research studies, foster collaborations, and build community.
One way in which we reach out to the community is by attending national and local fly group meetings. In 2018, this included or will include attendance at the ADRC fly meeting in Philly (spring 2018), and past or upcoming presentations at Flies on the Beach (FL),...
Thank you to all those who attended our workshop at last week's Annual Drosophila Research Conference in Philadelphia, PA, USA. It was great to talk fly stocks, cell screens, and bioinformatics with the community. We are here to help and look forward to continued feedback on the resources we are building to empower your research. PDFs of our workshop presentations are attached to this news item. The slides will help you learn more about our in vivo resources for CRISPR, new pooled cell-based CRISPR screen technology, and bioinformatics resources at our facility. Feel free to contact... Read more about Missed us at ADRC 2018? View our workshop slides!
The DRSC/TRiP-FGR is pleased to support collaborations on pooled CRISPR screens using the method recently, reported in eLife by Viswanatha et al. From the abstract: "... Here, we developed a site-specific integration strategy for library delivery and performed a genome-wide CRISPR knockout screen in Drosophila S2R+ cells. Under basal growth conditions, 1235 genes were essential for cell fitness at a false-discovery rate of 5...
The DRSC and TRiP will be represented at "the fly meeting" -- the GSA Annual Drosophila Research Converence -- in March of 2019. We look forward to attending this 60th ADRC to talk RNAi, CRISPR, and other technologies; present what's new in our three areas of focuse (cell-based studies, informatics, and in vivo studies); and provide consultation on specific projects. Please feel free to reach out if you want to be sure to connect with us at the meeting. See you in Dallas!
The DRSC & TRiP will be represented at the ASCB|EMBO 2018 conference in the form of a poster that includes information about our collaboration with O. Kanca in the H. Bellen lab at Baylor College of Medicine to tag Drosophila S2R+ cell lines with GFP using a CRISPR-based strategy. We have 'painted' a number of subcellular localizations green with this approach! If you're at the meeting, check out P1482 at Board Number B494, presented by Baolong Xia of the Perrimon lab.
The DRSC & TRiP will be represented at the Harvard Medical School showcase of facilities that provide research resources and services. The event takes place Nov. 29, 2018, at the Joseph B. Martin Conference Center in the New Research Building at 77 Avenue Louis Pasteur.
DRSC/TRiP-FGR Director S. Mohr will be presenting information about our resources to a regular meeting of Seattle-area fly labs on Nov. 8, 2018. Information about our cell-based technologies including CRISPR cell modification and screening; in vivo technologies including our growing collection of fly stocks for CRISPR knockout and activation; and our growing portfolio bioinformatics resources will be covered.