News & Events

News

Graphical image of tissue culture, fly pushing, and computer, and the team of people who work with them

Join our team!

May 30, 2018
We are hiring! We are looking for strong candidates for research technician positions at entry and senior levels, as well as for a strong candidate to join our bioinformatics team. Details on the Perrimon lab website. Questions can be directed to Stephanie Mohr, Jonathan Zirin, or Claire Yanhui Hu. Job inquiries and resumes can be sent to Jonathan Zirin. Read more about Join our team!

Missed us at ADRC 2018? View our workshop slides!

April 19, 2018
Thank you to all those who attended our workshop at last week's Annual Drosophila Research Conference in Philadelphia, PA, USA. It was great to talk fly stocks, cell screens, and bioinformatics with the community. We are here to help and look forward to continued feedback on the resources we are building to empower your research. PDFs of our workshop presentations are attached to this news item. The slides will help you learn more about our in vivo resources for CRISPR, new pooled cell-based CRISPR screen technology, and bioinformatics resources at our facility.  Feel free to contact... Read more about Missed us at ADRC 2018? View our workshop slides!
Cartoon of essential gene pooled screen (made using BioRender.io)

Pooled-format CRISPR screens in Drosophila cells

March 22, 2018

The DRSC/TRiP-FGR is pleased to support collaborations on pooled CRISPR screens using the method recently reported in a BioRxiv pre-print by Viswanatha et al. From the abstract: "... Here, we developed a site-specific integration strategy for library delivery and performed a genome-wide CRISPR knockout screen in Drosophila S2R+ cells. Under basal growth conditions, 1235 genes were essential for cell fitness at a false-discovery rate of 5...

Read more about Pooled-format CRISPR screens in Drosophila cells
Photo of 384-well assay plates

Congrats to Sung, Shears, and colleagues: "Cytokine signaling through Drosophila Mthl10 ties lifespan to environmental stress"

December 13, 2017

Eui Jae Sung, Stephen Shears, and colleagues have published a research report that includes a screen of dsRNAs from the DRSC reagent collection using S2 cells. We shipped dsRNA reagents to the lab for a screen at their home institution, in addition to providing consultation and data management support. The resulting study by Sung et al. was published on Dec. 11, 2017: Sung EJ, Ryuda M, Matsumoto H, Uryu O, Ochiai M, Cook ME, Yi NY, Wang H, Putney JW, Bird GS, Shears SB, Hayakawa Y. Cytokine signaling through Drosophila Mthl10 ties lifespan to environmental stress...

Read more about Congrats to Sung, Shears, and colleagues: "Cytokine signaling through Drosophila Mthl10 ties lifespan to environmental stress"
Figure 2 from Housden et al 2017 PNAS

Variable Dose Analysis: a new DRSC-supported cell screen approach that leverages existing reagents to perform robust screens

December 1, 2017

We are excited to report the publication of a paper from Benjamin Housden and colleagues describing development and use of the Variable Dose Analysis (VDA) approach. Ben developed a way to use existing TRiP shRNA plasmids originally developed for fly stock production in a new, effective approach to high-throughput cell screening.

The VDA approach is particularly useful for combinatorial approaches that are acutely sensitive to assay robustness. The screen Ben and colleagues report focused on synthetic effects in Drosophila tumor model cells. ...

Read more about Variable Dose Analysis: a new DRSC-supported cell screen approach that leverages existing reagents to perform robust screens
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Events

2018 Nov 08

DRSC/TRiP-FGR Director S. Mohr to present on our resources to Seattle-area fly labs

(All day)

Location: 

Seattle, WA
DRSC/TRiP-FGR Director S. Mohr will be presenting information about  our resources to a regular meeting of Seattle-area fly labs on Nov. 8, 2018. Information about our cell-based technologies including CRISPR cell modification and screening; in vivo technologies including our growing collection of fly stocks for CRISPR knockout and activation; and our growing portfolio bioinformatics resources will be covered.
2018 Sep 28

DRSC/TRiP-FGR Director S. Mohr to present at U Mass Worcester

12:00pm to 1:00pm

Location: 

Worcester, MA
DRSC/TRiP-FGR Director, Dr. Stephanie Mohr, will be presenting information about our three areas of focus -- cell-based screens, in vivo fly stock production, and bioinformatics -- to a group of fly labs at U Mass Worcester. Feel free to contact Stephanie if you are interested to have a similar presentation to a group of fly labs in your area. We can offer slide presentations, bioinformatics hands-on tutorials, Q&A, or other visits to help you learn about our resources and use them to the fullest.
2018 Jun 28

DRSC/TRiP-FGR Director S. Mohr to present at Brown University's Fly Club

12:00pm to 1:00pm

Location: 

Providence, RI
DRSC/TRiP-FGR Director, Dr. Stephanie Mohr, will be presenting information about our three areas of focus -- cell-based screens, in vivo fly stock production, and bioinformatics -- to Brown Unviersity's Fly Club, a graduate student-run meeting of fly groups at Brown. Feel free to contact Stephanie if you are interested to have a similar presentation to a group of fly labs in your area. We can offer slide presentations, bioinformatics hands-on tutorials, Q&A, or other visits to help you learn about our resources and use them to the fullest.
2018 Jun 06

Boston Area Drosophila Meeting

(All day)

Location: 

Boston, MA
The DRSC/TRiP is hosting the Boston Area Drosophila Meeting on Wed. June 6th. The agenda is available here as a PDF file download.
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