News & Events

News

sgRNA tracking

The TRiP is now taking nominations for CRISPR stocks

May 12, 2017

The TRiP is excited to announce our new TRiP-CRISPR transgenic fly libraries for gene knockout and overexpression. Visit the sgRNA tracking site to download and search existing TRiP-CRISPR fly stocks by gene or stock ID to obtain information on sgRNA sequence, function, vector, injection site, and availablility.

The database also has a nominations page that serves as the online access point for the public to nominate genes for eith… Read more about The TRiP is now taking nominations for CRISPR stocks

Screenshot of a 2015 Science paper from Payre and colleagues

Francois Payre's plenary talk at ADRC 2017 features results from DRSC cell-based screen

March 30, 2017

Those of us lucky enough to be at the Annual Drosophila Research Conference this morning saw a great talk by Francois Payre about regulation of Shavenbaby by small ORFs. A genome-wide cell-based screen done at the DRSC by Emilie Benrabah identified the mechanism of regulation. As this exemplifies, cell screens can help identify key pathways and factors that can then be followed up with in vivo studies.

New stocks added to the TRiP in vivo RNAi library

January 6, 2017

The TRiP has updated and curated our list of in vivo RNAi reagents for gene knockdown in fruitflies. To date, we have produced over 13,000 stocks for the benefit of the scientific community.

Visit the in vivo RNAi fly stocks and vectors page to download an excel file with the full list of fly stocks now available for order from the Bloomington Drosophila Stock Center (BDSC) and the National Institute of Genetics, Japan (NIG-Fly).

 

CRISPR sgRNA design tool now based on Drosophila genome assembly 6

December 21, 2016

We have updated our CRISPR sgRNA design tool. The results and JBrowse display are now based on Drosophila melanogaster FlyBase genome assembly release 6. We have also added seed scores in addition to efficiency prediction scores and other values available on detailed views of sgRNA designs. Quick tips: start a search with a gene symbol or other identifier, use the "choose tracks" tab to view options and choose your settings, then view designs (little green… Read more about CRISPR sgRNA design tool now based on Drosophila genome assembly 6

Amino acid alignment of the fly paralogs kek1 and kek2

DIOPT 6.0 released -- with eggNOG and paralog searches added

November 29, 2016

DIOPT 6.0 went live this week. Newly added features include results from eggNOG, bringing the total number of alrogithms incorporated in our integrated search tool to 14. In addition, you can now search for paralogs. To do this, choose the same species for input and output. Examples for fly-fly and human-human paralog searches are shown. As always, your feedback is welcome.

Read more about DIOPT 6.0 released -- with eggNOG and paralog searches added

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Events

2017 May 10

Presentation at Genome Editing and Trangenic Congress USA

10:00am

Location: 

Boston, MA

Stephanie will be presenting at the Genome Editing and Transgenic Congress USA in Boston, MA, on May 10, 2017 (time TBD). She plans to cover the following topics.

  • Strategy and results of optimization of Cas9 fusion proteins for CRISPR activation in cells and in vivo
  • Genome-scale design of single guide RNAs (sgRNAs) for CRISPR activation
  • Use of CRISPR activation in Drosophila for large-scale gene function discovery and disease modeling
2017 Mar 29

DRSC & TRiP at the fly meeting

Mar 29 (All day) to Apr 02 (All day)

Location: 

San Diego, CA

The DRSC and TRiP are represented at the fly meeting! Stop by posters 712A and 709A to learn about new technologies and online resources. Photos of the posters are welcome!

2017 Mar 29

DRSC & TRiP at ADRC 2017

Mar 29 (All day) to Apr 02 (All day)

Location: 

San Diego, CA

The DRSC & TRiP will be at the Annual Drosophila Research Conference (aka 'the fly meeting') in San Diego, CA, in March/April of 2017. Please feel free to contact us if you'd like to set up a meeting to discuss a screen project or other research need we can help with.

2016 Dec 01

Harvard Med School Research Core Facilities Showcase

3:30pm to 5:30pm

Location: 

TMEC Atrium on the Harvard Medical School campus

The DRSC & TRiP will be represented at the HMS Research Cores Showcase. The event will be comprised of a poster session and reception. The event provides an opportunity for HMS students, postdocs, and faculty can get to know what offerings are available from facilities on the campus. If you are local to HMS, drop by to say hello or ask questions about our offerings in the area of cell-based screening, RNAi and CRISPR fly stocks, and bioinformatics.

2016 Sep 28

Functional genomics techniques in Drosophila and their potential application in non-model insects

11:45am to 12:00pm

Location: 

Orlando, FL

DRSC-FGR Director S. Mohr will be presenting in the symposium Insect Genetic Technologies: State of the Art and Promise for the Future at the International Congress of Entomology (ICE 2016). Come hear what is possible in Drosophila that might be applied to other insect species. Wednesday, September 28, 2016 at 11:45 am (symposium from 9:30 - 12:30).

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