The TRiP is excited to announce our new TRiP-CRISPR transgenic fly libraries for gene knockout and overexpression. Visit the sgRNA tracking site to download and search existing TRiP-CRISPR fly stocks by gene or stock ID to obtain information on sgRNA sequence, function, vector, injection site, and availablility.
Those of us lucky enough to be at the Annual Drosophila Research Conference this morning saw a great talk by Francois Payre about regulation of Shavenbaby by small ORFs. A genome-wide cell-based screen done at the DRSC by Emilie Benrabah identified the mechanism of regulation. As this exemplifies, cell screens can help identify key pathways and factors that can then be followed up with in vivo studies.
The DRSC/TRiP-FGR is pleased to announce that we recently updated our high-throughput, high-content imaging system. Through funds from NIH, HHMI, and the Harvard Medical School Tools & Technology program, we were able to get a GE IN Cell 6000 automated imaging system.
The TRiP has updated and curated our list of in vivo RNAi reagents for gene knockdown in fruitflies.To date, we have produced over 13,000 stocks for the benefit of the scientific community.
Visit the in vivo RNAi fly stocks and vectors page to download an excel file with the full list of fly stocks now available for order from the BloomingtonDrosophila Stock Center (BDSC) and the National Institute of Genetics, Japan (NIG-Fly).
DIOPT 6.0 went live this week. Newly added features include results from eggNOG, bringing the total number of alrogithms incorporated in our integrated search tool to 14. In addition, you can now search for paralogs. To do this, choose the same species for input and output. Examples for fly-fly and human-human paralog searches are shown. As always, your feedback is welcome.
On Sept. 27, 2017, DRSC/TRiP-FGR Director S. Mohr will present a talk on "High-throughput CRISPR-based approaches to cancer target identification" at the 2017 CHI Discovery on Target conference at the Westin Copley Place, Boston, MA, USA.
The DRSC & TRiP will be at the Annual Drosophila Research Conference (aka 'the fly meeting') in San Diego, CA, in March/April of 2017. Please feel free to contact us if you'd like to set up a meeting to discuss a screen project or other research need we can help with.
The DRSC & TRiP will be represented at the HMS Research Cores Showcase. The event will be comprised of a poster session and reception. The event provides an opportunity for HMS students, postdocs, and faculty can get to know what offerings are available from facilities on the campus. If you are local to HMS, drop by to say hello or ask questions about our offerings in the area of cell-based screening, RNAi and CRISPR fly stocks, and bioinformatics.