We are hiring! We are looking for strong candidates for research technician positions at entry and senior levels, as well as for a strong candidate to join our bioinformatics team. Details on the Perrimon lab website. Questions can be directed to Stephanie Mohr, Jonathan Zirin, or Claire Yanhui Hu. Job inquiries and resumes can be sent to Jonathan Zirin. Read more about Join our team!

Thank you to all those who attended our workshop at last week's Annual Drosophila Research Conference in Philadelphia, PA, USA. It was great to talk fly stocks, cell screens, and bioinformatics with the community. We are here to help and look forward to continued feedback on the resources we are building to empower your research. PDFs of our workshop presentations are attached to this news item. The slides will help you learn more about our in vivo resources for CRISPR, new pooled cell-based CRISPR screen technology, and bioinformatics resources at our facility.  Feel free to contact... Read more about Missed us at ADRC 2018? View our workshop slides!

The DRSC/TRiP-FGR is pleased to support collaborations on pooled CRISPR screens using the method recently reported in a BioRxiv pre-print by Viswanatha et al. From the abstract: "... Here, we developed a site-specific integration strategy for library delivery and performed a genome-wide CRISPR knockout screen in Drosophila S2R+ cells. Under basal growth conditions, 1235 genes were essential for cell fitness at a false-discovery rate of 5...

Read more about Pooled-format CRISPR screens in Drosophila cells

We are looking for strong candidates for a research assistant position at the DRSC/TRiP Functional Genomics Resources. Be sure to note in your resume if you have experience with Drosophila genetics or cell culture. Details and information about how to apply here. Read more about We're hiring at the research assistant level

Eui Jae Sung, Stephen Shears, and colleagues have published a research report that includes a screen of dsRNAs from the DRSC reagent collection using S2 cells. We shipped dsRNA reagents to the lab for a screen at their home institution, in addition to providing consultation and data management support. The resulting study by Sung et al. was published on Dec. 11, 2017: Sung EJ, Ryuda M, Matsumoto H, Uryu O, Ochiai M, Cook ME, Yi NY, Wang H, Putney JW, Bird GS, Shears SB, Hayakawa Y. Cytokine signaling through Drosophila Mthl10 ties lifespan to environmental stress...

Read more about Congrats to Sung, Shears, and colleagues: "Cytokine signaling through Drosophila Mthl10 ties lifespan to environmental stress"

We are excited to report the publication of a paper from Benjamin Housden and colleagues describing development and use of the Variable Dose Analysis (VDA) approach. Ben developed a way to use existing TRiP shRNA plasmids originally developed for fly stock production in a new, effective approach to high-throughput cell screening.

The VDA approach is particularly useful for combinatorial approaches that are acutely sensitive to assay robustness. The screen Ben and colleagues report focused on synthetic effects in Drosophila tumor model cells. ...

Read more about Variable Dose Analysis: a new DRSC-supported cell screen approach that leverages existing reagents to perform robust screens