At the DRSC-BTRR, we've been doing a lot of pooled-format CRISPR knockout screens in Drosophila cells. We're finding the results to be robust and reproducible. And best of all, the results have been informative, providing insights into diverse areas of biology.
Thinking about how to do CRISPR knockout screens in cells is a little different from thinking about how to do a genetic or RNAi screen in vivo or doing an arrayed-format RNAi screen....
Below we share with the community at large the report we provided to the Fly Board in advance of the 2022 Fly Meeting. As always, your feedback on our technologies and services is welcome. We are here to help.
DRSC/TRiP Functional Genomics Resources at Harvard Medical School
Do you have questions about modifying Drosophila cell lines with CRISPR or performing large-scale cell screens? Questions about in vivo RNAi with TRiP fly stocks or CRISPR knockout or activation with our sgRNA fly stocks? Questions about our new protocols and resources for CRISPR mosquito cell lines? Pop into our Zoom office hours to say hello and get our expert input! Registration is required (see below).
The DRSC bioinformatics team, led by Dr. Claire Yanhui Hu, has recently published two new papers.
One reports development of BioLitMine, an advanced literature mining resource. The other provides an overview of our online resources, which can be grouped into reagent, gene, and data-focused resources.
Did you miss the presentations from Claire Hu and Jonathan Zirin at the June 2020 Boston Area Drosophila Meeting? No problem! The slides can be accessed from this post. Click the title above to view the whole post, then scroll down to access the PDFs. These presentations describe what's new and next in bioinformatics and in vivo technologies at the DRSC/TRiP. Feel free to reach out with questions. Interested in the BAD meeting? Info about the meeting can be found here. Read more about DRSC/TRiP presentations from June 2020 Boston Area Drosophila Meeting
The DRSC-Biomedical Technology Research Resource will present a 2-minute introduction to the group's efforts in bioinformatics and cell-based resources for mosquito research at the beginning of the March 18th meeting of the Virtual Vector Biology seminar series.