Thank you to all those who attended our workshop at last week's Annual Drosophila Research Conference in Philadelphia, PA, USA. It was great to talk fly stocks, cell screens, and bioinformatics with the community. We are here to help and look forward to continued feedback on the resources we are building to empower your research. PDFs of our workshop presentations are attached to this news item. The slides will help you learn more about our in vivo resources for CRISPR, new pooled cell-based CRISPR screen technology, and bioinformatics resources at our facility. Feel free to contact... Read more about Missed us at ADRC 2018? View our workshop slides!
The DRSC/TRiP-FGR is pleased to support collaborations on pooled CRISPR screens using the method recently reported in a BioRxiv pre-print by Viswanatha et al. From the abstract: "... Here, we developed a site-specific integration strategy for library delivery and performed a genome-wide CRISPR knockout screen in Drosophila S2R+ cells. Under basal growth conditions, 1235 genes were essential for cell fitness at a false-discovery rate of 5...
Eui Jae Sung, Stephen Shears, and colleagues have published a research report that includes a screen of dsRNAs from the DRSC reagent collection using S2 cells. We shipped dsRNA reagents to the lab for a screen at their home institution, in addition to providing consultation and data management support. The resulting study by Sung et al. was published on Dec. 11, 2017: Sung EJ, Ryuda M, Matsumoto H, Uryu O, Ochiai M, Cook ME, Yi NY, Wang H, Putney JW, Bird GS, Shears SB, Hayakawa Y. Cytokine signaling through Drosophila Mthl10 ties lifespan to environmental stress...
We are excited to report the publication of a paper from Benjamin Housden and colleagues describing development and use of the Variable Dose Analysis (VDA) approach. Ben developed a way to use existing TRiP shRNA plasmids originally developed for fly stock production in a new, effective approach to high-throughput cell screening.
The VDA approach is particularly useful for combinatorial approaches that are acutely sensitive to assay robustness. The screen Ben and colleagues report focused on synthetic effects in Drosophila tumor model cells. ...
DRSC/TRiP-FGR Director S. Mohr will be presenting information about our resources to a regular meeting of Seattle-area fly labs on Nov. 8, 2018. Information about our cell-based technologies including CRISPR cell modification and screening; in vivo technologies including our growing collection of fly stocks for CRISPR knockout and activation; and our growing portfolio bioinformatics resources will be covered.
DRSC/TRiP-FGR Director, Dr. Stephanie Mohr, will be presenting information about our three areas of focus -- cell-based screens, in vivo fly stock production, and bioinformatics -- to a group of fly labs at U Mass Worcester. Feel free to contact Stephanie if you are interested to have a similar presentation to a group of fly labs in your area. We can offer slide presentations, bioinformatics hands-on tutorials, Q&A, or other visits to help you learn about our resources and use them to the fullest.
DRSC/TRiP-FGR Director, Dr. Stephanie Mohr, will be presenting information about our three areas of focus -- cell-based screens, in vivo fly stock production, and bioinformatics -- to Brown Unviersity's Fly Club, a graduate student-run meeting of fly groups at Brown. Feel free to contact Stephanie if you are interested to have a similar presentation to a group of fly labs in your area. We can offer slide presentations, bioinformatics hands-on tutorials, Q&A, or other visits to help you learn about our resources and use them to the fullest.
The DRSC & TRiP will be hosting a workshop at the Annual Drosophila Research Conference in Philadelphia, PA. The workshop is scheduled for Friday, April 13th from 1:45 to 3:45 PM. Come hear from DRSC & TRiP leaders Norbert Perrimon, Jonathan Zirin (organizer), Claire Yanhui Hu, and Stephanie Mohr. At the workshop, you will learn about new opportunities for community nomination and experiments using CRISPR knockout and activation, as well as learn what's new and popular among our online software and database tools. There will be something for everyone -- we will provide information... Read more about DRSC & TRiP Workshop at ADRC
The DRSC & TRiP leadership consider the Annual Drosophila Research Conference the 'must-go' conference of the year. We use ADRC meetings as an opportunity to update the community on existing and new services, reagent collections, and online tools, as well as for one-on-one consults with researchers planning high-throughput screens. The next ADRC is scheduled for April 11-15. 2018, in Philadelphia, PA, USA. Feel free to contact us if you'll also be there and would... Read more about 59th Annual Drosophila Research Conference