Single-cell isolation

2018 Dec 08

DRSC&TRiP at ASCB|EMBO 2018

Sat Dec 8 (All day) to Wed Dec 12 (All day)

Location: 

San Diego, CA, USA
The DRSC & TRiP will be represented at the ASCB|EMBO 2018 conference in the form of a poster that includes information about our collaboration with O. Kanca in the H. Bellen lab at Baylor College of Medicine to tag Drosophila S2R+ cell lines with GFP using a CRISPR-based strategy. We have 'painted' a number of subcellular localizations green with this approach! If you're at the meeting, check out P1482 at Board Number B494, presented by Baolong Xia of the Perrimon lab.
Benjamin E Housden, Shuailiang Lin, and Norbert Perrimon. 2014. “Cas9-based genome editing in Drosophila.” Methods Enzymol, 546, Pp. 415-39.Abstract

Our ability to modify the Drosophila genome has recently been revolutionized by the development of the CRISPR system. The simplicity and high efficiency of this system allows its widespread use for many different applications, greatly increasing the range of genome modification experiments that can be performed. Here, we first discuss some general design principles for genome engineering experiments in Drosophila and then present detailed protocols for the production of CRISPR reagents and screening strategies to detect successful genome modification events in both tissue culture cells and animals.