A genome-scale shRNA resource for transgenic RNAi in Drosophila.


Jian-Quan Ni, Rui Zhou, Benjamin Czech, Lu-Ping Liu, Laura Holderbaum, Donghui Yang-Zhou, Hye-Seok Shim, Rong Tao, Dominik Handler, Phillip Karpowicz, Richard Binari, Matthew Booker, Julius Brennecke, Lizabeth A Perkins, Gregory J Hannon, and Norbert Perrimon. 2011. “A genome-scale shRNA resource for transgenic RNAi in Drosophila.” Nat Methods, 8, 5, Pp. 405-7.
2011_Nat Met_Ni.pdf908 KB
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Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.

Last updated on 10/06/2016