#  UAS overexpression 

 



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## Why overexpress a gene?

In many cases overexpression of a gene product can produce informative mutant phenotypes. Overexpression can also provide a useful approach to epistasis assays and screening. The TRiP developed an overexpression approach to meet community needs.

## Our overexpression approach

- is based on our previous strategy for knockdown vector design
- includes *vermilion* (VALIUM) and *mini-white* (WALIUM) visible marker versions of VALIUM10
- includes two different cloning strategies, 'roe' for recombination and 'moe' for multiple cloning site-containing overexpression vectors
- the two markers and two cloning strategies result in four differen options for overxpression constructs:
    - pVALIUM10-roe, pVALIUM10-moe, pWALIUM10-roe, pWALIUM10-moe
- to learn more visit the [TRiP plasmid vector sets](/trip-plasmid-vector-sets) page
- to obtain these vectors, visit the [plasmid repository of the DF/HCC DNA Resource Core](https://plasmid.med.harvard.edu/PLASMID/)