#  TRiP-CRISPR Overexpression (TRiP-OE) 

 



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## For activation of target gene expression


## TRiP-OE: The flySAM2.0 Collection

- New simplified strategy for *in vivo* CRISPR activation
- flySAM generates stronger overexpression phenotypes, comparable to Gal4/UAS
- Activate expression of target gene by crossing to any Gal4 line

 ![flySAM](/sites/g/files/omnuum5366/files/fly/files/tripoe_sam.png)

 



## TRiP-OE: The VPR Collection

- Activate expression of target gene by crossing to Gal4>dCas9-VPR flies
- Generate InDels and larger genomic deletions by crossing to Gal4>Cas9 flies
- For a detailed description of the the TRiP-OE gene activation approach click [here](/vivo-crispr-0)

 ![VPR](/sites/g/files/omnuum5366/files/fly/files/tripoe_vpr.png)

 


## About TRiP-OE stocks

### flySAM

- A single gene is targeted by expression of one sgRNA from the U6:2 promoter
- Stocks are made in the flySAM2.0 vector, developed by Jian-quan Ni and colleagues
- TRiP-OE/flySAM stocks contain UAS-Cas9, so simply crossing to a Gal4 induces expression of the target gene
- To learn more about the TRiP-OE vectors, sgRNA design, and gene activation please visit the [in vivo CRISPR protocols page](/vivo-crispr-0)

### VPR

- A single gene is targeted by tandem expression of two sgRNAs from independent U6 promoters
- Stocks are made in the pCFD4 vector, developed by Fillip Port and colleagues (<http://www.crisprflydesign.org/plasmids>)
- Crossing TRiP-OE stocks to a Gal4 line expressing dCas9 fused to the chimeric activator domain VPR induces expression of the target gene
- To learn more about the TRiP-OE vectors, sgRNA design, and gene activation please visit the [in vivo CRISPR protocols page](/vivo-crispr-0)

## Access TRiP-OE stocks

- Order TRiP-OE transgenic stocks at the [Bloomington Drosophila Stock Center](https://bdsc.indiana.edu/stocks/genome_editing/sgrna.html) (BDSC)
- If a TRiP-CRISPR stock has not yet been deposited at the BDSC, please contact the [TRiP facility at HMS](mailto:///TRiP@flyrnai.org) and we will send it to you
- [**Download**](http://www.flyrnai.org/tools/grna_tracker/web/sgRNA_finished_stocks.csv) a .XLS file of currently available TRiP-CRISPR stocks

## Search TRiP-OE stocks

- [**Search** the DRSC/TRiP sgRNA Stock Tracking System](http://www.flyrnai.org/tools/grna_tracker/web/) for TRiP-OE stocks and production status, and [**nominate genes for productio**n](http://www.flyrnai.org/tools/grna_tracker/web/nominate)