#  TRiP-CRISPR Knockout (TRiP-KO) 

 



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## For target gene mutagenesis

- Generate indels (small insertion or deletion mutations) in the soma by crossing to tissue-specific Gal4>Cas9 flies
- Generate indels in the germline by crossing to germline-specific-Cas9 flies
- For a detailed description of the TRiP-KO gene mutagenesis approach click [here](/vivo-crispr-0)

 ![TRiP-KO](/sites/g/files/omnuum5366/files/fly/files/trip_ko.png)

 

## Access TRiP-KO stocks

- Order TRiP-KO transgenic stocks at the [Bloomington Drosophila Stock Center](https://bdsc.indiana.edu/stocks/genome_editing/sgrna.html) (BDSC)
- If a TRiP-CRISPR stock has not yet been deposited at the BDSC, please contact the [TRiP facility at HMS](mailto:///TRiP@flyrnai.org) and we will send it to you
- [**Download**](https://www.flyrnai.org/tools/grna_tracker/web/sgRNA_finished_stocks.csv) a .XLS file of currently available TRiP-CRISPR stocks

## Search TRiP-KO stocks

- [**Search** the DRSC/TRiP sgRNA Stock Tracking System](http://www.flyrnai.org/tools/grna_tracker/web/) for TRiP-KO stocks and production status, and [**nominate genes for production**](http://www.flyrnai.org/tools/grna_tracker/web/nominate)

## About TRiP-KO stocks

- A single gene is targeted by expression of a single sgRNA from a U6 promoter
- Stocks are made in the pCFD3 vector, developed by Fillip Port and colleagues (<http://www.crisprflydesign.org/plasmids>)
- Crossing TRiP KO stocks to a Gal4 line expressing Cas9 induces cleavage of the target gene and mutagenesis via Non-Homologous End Joining (NHEJ) repair
- To learn more about the TRiP KO vectors, sgRNA design, and CRISPR mutagenesis please visit the [in vivo CRISPR protocols page](/vivo-crispr-0)
- For the benefit of the research community, production of TRiP-KO stocks is coordinated with similar efforts at the German Cancer Research Center (<http://www.crisprflydesign.org/>) and The National Institute of Genetics, Japan (<https://shigen.nig.ac.jp/fly/nigfly/cas9/>)