# in vivo RNAi fly stocks and vectors ## expand\_more To date the TRiP has generated over 12,000 Drosophila RNAi stocks, and developed efficient vectors for RNAi that work in all tissues. For a detailed description of the the TRiP in vivo RNAi approach click [here](/trip-vivo-rnai-approach). ## TRiP fly stock collections - [TRiPSoma](/trip-rnai-fly-stocks)- for effective knockdown in somatic cells - [TRiPGermline](/trip-rnai-fly-stocks)- for effective knockdown in the germline - [Human Disease Transgenic RNAi Project (HuDis-TRiP)](/hudis-trip-fly-stocks) - targeting orthologs of genes associated with human diseases - [TRiP Toolbox](/trip-toolbox) - injection stocks for labs wishing to generate their own RNAi lines and commonly used GAL4 lines with UAS-Dcr2 to enhance knockdown - [Controls](/trip-rnai-control-fly-stocks)- TRiP stocks for use as experimental controls - [Current TRiP stocks (xlsx) ](/file_url/734), including detailed information on hairpin design and target(s) ## Community Access to the TRiP Stocks The Transgenic RNAi Project continues to make new RNAi fly stocks for the community and to maintain and improve the current library of [TRiP RNAi stocks available at BDSC](http://flystocks.bio.indiana.edu/Browse/RNAi/RNAihome.htm). - All completed stocks are annotated on the TRiP website and on FlyBase, and transferred as soon as possible to the BDSC for distribution to the community - If a TRiP stock has not yet been deposited at the BDSC, please contact the [TRiP facility at HMS](mailto:///TRiP@flyrnai.org) and we will send it to you - If use of any of the TRiP stocks results in data that is included in a manuscript for publication, the TRiP requests that a version of the following statement be included in the Acknowledgements section: "We thank the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for providing transgenic RNAi fly stocks used in this study" ## Nominating genes for TRiP production - Selections will be in keeping with the BDSC mandate of one mutation per gene - We will meet the needs of screeners at the Drosophila RNAi Screening Center (DRSC) for in vivo follow-up studies subsequent to cell-based screens - We will meet the needs of the Drosophila community for in vivo phenotypic analyses through a nomination process. In keeping with our NIH/NIGMS funding, priority will go to nominations that help to fill in the phenotype gap and overcome issues associated with pleiotropy To nominate a gene(s), the PI of the lab should send a letter to that includes an explanation of why the specific gene is being nominated. ## TRiP vectors - [Knockdown vectors](/trip-plasmid-vector-sets) - 1st and 2nd generation VALIUM and WALIUM vectors and QUAS vector for transgenic RNAi - [Overexpression vectors](/trip-plasmid-vector-sets) - VALIUM10-moe, VALIUM10-roe, WALIUM10-moe, and WALIUM10-roe for in vivo overexpression. - [mENTRY](/trip-plasmid-vector-sets) - a Gateway donor/entry vector with multiple cloning site - TRiP vectors, including vermillion and white versions of vectors for over-expression, are available through the Drosophila Genome Resource Center (DGRC) in Bloomington, IN, USA. Look for [VALIUM and WALIUM plasmids in their online vectors catalog](https://dgrc.bio.indiana.edu/vectors/Catalog). ## Publications Download 6 citations download- [BibTeX](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=tagged) - [Marc](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=marc) - [PubMedId](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=no_pager&number_of_items=6&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_technology%5D%5B0%5D%5Btarget_id%5D=114101&&&format=ris) ### 2021 Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “[Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila](/publications/genome-wide-vitro-and-vivo-rnai-screens-reveal-fer3-be-important-regulator-kkv)”. Insect Sci. doi:10.1111/1744-7917.12954 Xiangzhao Yue, Yongkang Liang, Zhishuang Wei, Jun Lv, Yongjin Cai, Xiaobin Fan, Wenqing Zhang, and Jie Chen. 2021. “[Genome-Wide in Vitro and in Vivo RNAi Screens Reveal Fer3 to Be an Important Regulator of Kkv Transcription in Drosophila](/publications/genome-wide-vitro-and-vivo-rnai-screens-reveal-fer3-be-important-regulator-kkv)”. Insect Sci. doi:10.1111/1744-7917.12954 - add\_circle do\_not\_disturb\_on Abstract Krotzkopf verkehrt (kkv) is a key enzyme that catalyzes the synthesis of chitin, an important component of the Drosophila epidermis, trachea, and other tissues. Here, we report the use of comprehensive RNA interference (RNAi) analyses to search for kkv... ### 2020 Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “[FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update](/publications/flyrnaiorg-database-drosophila-rnai-screening-center-and-transgenic-rnai-project)”. Nucleic Acids Res. doi:10.1093/nar/gkaa936 Yanhui Hu, Aram Comjean, Jonathan Rodiger, Yifang Liu, Yue Gao, Verena Chung, Jonathan Zirin, Norbert Perrimon, and Stephanie Mohr. 2020. “[FlyRNAi.Org-the Database of the Drosophila RNAi Screening Center and Transgenic RNAi Project: 2021 Update](/publications/flyrnaiorg-database-drosophila-rnai-screening-center-and-transgenic-rnai-project)”. Nucleic Acids Res. doi:10.1093/nar/gkaa936 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdfgkaa936.pdf](/sites/g/files/omnuum5366/files/fly/files/gkaa936.pdf) The FlyRNAi database at the Drosophila RNAi Screening Center and Transgenic RNAi Project (DRSC/TRiP) provides a suite of online resources that facilitate functional genomics studies with a special emphasis on Drosophila melanogaster. Currently, the... - [ picture\_as\_pdfgkaa936.pdf](/sites/g/files/omnuum5366/files/fly/files/gkaa936.pdf) Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “[Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies](/publications/large-scale-transgenic-resource-collections-loss-and-gain-function-studies)”. Genetics. doi:10.1534/genetics.119.302964 Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Ping Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie Mohr, Jianquan Ni, and Norbert Perrimon. 2020. “[Large-Scale Transgenic Resource Collections for Loss- and Gain-of-Function Studies](/publications/large-scale-transgenic-resource-collections-loss-and-gain-function-studies)”. Genetics. doi:10.1534/genetics.119.302964 - add\_circle do\_not\_disturb\_on Abstract The Transgenic RNAi Project (TRiP), a functional genomics platform at Harvard Medical School, was initiated in 2008 to generate and distribute a genome-scale collection of RNAi fly stocks. To date, the TRiP has generated >15,000 RNAi fly stocks. As this... ### 2019 Michael Rotelli, Anna Bolling, Andrew Killion, Abraham Weinberg, Michael Dixon, and Brian Calvi. 2019. “[An RNAi Screen for Genes Required for Growth of Wing Tissue](/publications/rnai-screen-genes-required-growth-wing-tissue)”. G3 (Bethesda), 9, 10, Pp. 3087-3100. doi:10.1534/g3.119.400581 Michael Rotelli, Anna Bolling, Andrew Killion, Abraham Weinberg, Michael Dixon, and Brian Calvi. 2019. “[An RNAi Screen for Genes Required for Growth of Wing Tissue](/publications/rnai-screen-genes-required-growth-wing-tissue)”. G3 (Bethesda), 9, 10, Pp. 3087-3100. doi:10.1534/g3.119.400581 - add\_circle do\_not\_disturb\_on Abstract - [ picture\_as\_pdf3087.full\_.pdf](/sites/g/files/omnuum5366/files/fly/files/3087.full_.pdf) Cell division and tissue growth must be coordinated with development. Defects in these processes are the basis for a number of diseases, including developmental malformations and cancer. We have conducted an unbiased RNAi screen for genes that are... - [ picture\_as\_pdf3087.full\_.pdf](/sites/g/files/omnuum5366/files/fly/files/3087.full_.pdf) Stephanie E. Mohr and Norbert Perrimon. 2019. “[Drosophila Melanogaster: A Simple System for Understanding Complexity.](/publications/drosophila-melanogaster-simple-system-understanding-complexity)”. Dis Model Mech, 12, 10 Stephanie E. Mohr and Norbert Perrimon. 2019. “[Drosophila Melanogaster: A Simple System for Understanding Complexity.](/publications/drosophila-melanogaster-simple-system-understanding-complexity)”. Dis Model Mech, 12, 10 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://dmm.biologists.org/content/12/10/dmm041871.long) - [ picture\_as\_pdf2019\_DMM\_Mohr.pdf](/sites/g/files/omnuum5366/files/fly/files/2019_DMM_Mohr.pdf) Understanding human gene function is fundamental to understanding and treating diseases. Research using the model organism *Drosophila melanogaster* benefits from a wealth of molecular genetic resources and information useful for efficient *in* *vivo* experimen... - [ descriptionPublisher's Version](https://dmm.biologists.org/content/12/10/dmm041871.long) - [ picture\_as\_pdf2019\_DMM\_Mohr.pdf](/sites/g/files/omnuum5366/files/fly/files/2019_DMM_Mohr.pdf) ### 2016 Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “[Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences](/publications/loss-function-genetic-tools-animal-models-cross-species-and-cross-platform)”. Nat Rev Genet Benjamin Housden, Matthias Muhar, Matthew Gemberling, Charles Gersbach, Didier YR Stainier, Geraldine Seydoux, Stephanie Mohr, Johannes Zuber, and Norbert Perrimon. 2016. “[Loss-of-Function Genetic Tools for Animal Models: Cross-Species and Cross-Platform Differences](/publications/loss-function-genetic-tools-animal-models-cross-species-and-cross-platform)”. Nat Rev Genet - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.ncbi.nlm.nih.gov/pubmed/27795562) - [ picture\_as\_pdf2016\_Nat Rev Gene\_Housden...](/sites/g/files/omnuum5366/files/fly/files/2016_NatRevGene_Housden.pdf) Our understanding of the genetic mechanisms that underlie biological processes has relied extensively on loss-of-function (LOF) analyses. LOF methods target DNA, RNA or protein to reduce or to ablate gene function. By analysing the phenotypes that are... - [ descriptionPublisher's Version](https://www.ncbi.nlm.nih.gov/pubmed/27795562) - [ picture\_as\_pdf2016\_Nat Rev Gene\_Housden...](/sites/g/files/omnuum5366/files/fly/files/2016_NatRevGene_Housden.pdf) [ More arrow\_circle\_right ](/publications/technology/in-vivo-fly-rnai)