# TRiP-CRISPR toolbox fly stocks ## expand\_more ## Cas9 stocks for use with [TRiP-CRISPR](/crispr-fly-stocks-and-vectors) sgRNA fly stock collections ## Completed TRiP-CRISPR/CAS9 Toolbox stocks are available at the [BDSC](https://bdsc.indiana.edu/stocks/genome_editing/triptoolbox_grna.html). ## Click [here](/file_url/647) to download list in .XLS format or see below Gal4+VPR (tissue-specific gene overexpression) Gal4+Cas9 (for tissue specific gene KO) nanos-Cas9 (for gene KO in germline) ## Gal4+VPR (tissue-specific gene overexpression) ![TRiP-OE VPR cross](/sites/g/files/omnuum5366/files/fly/files/screen_shot_2018-07-20_at_9.35.19_am.png) **Cross Gal4+dCas9-VPR stocks to sgRNA stocks from the [TRiP-OE VPR collection](/trip-overexpression-stocks) (sgRNAs cloned into pCFD4) to overexpress target gene.** GAL4/UAS expression of Cas9 proteins with dead nuclease activity (dCas9), fused to VPR transcriptional activator (dCas9-VPR). Not compatible with TRiP-OE sgRNA stocks from the flySAM collections (sgRNAs cloned into U6BsgRNA2.0 or flySAM2.0). Sort ### Gene activation - cross to TRiP-OE sgRNA stocks (VPR colletion) Nickname Availability Genotype elav>VPR in progress P{w[+mW.hs]=GawB}elav[C155];P{UAS-3XFLAG-dCas9-VPR}attp40/CyO MS1096>VPR [67039](https:/bdsc.indiana.edu/stocks/67039) w[1118] P{w[+mW.hs]=GawB}Bx[MS1096];P{UAS-3XFLAG-dCas9-VPR}attp40/CyO pnr>VPR [67040](https:/bdsc.indiana.edu/stocks/67040) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/(TM6B,Tb[1]) dMef2 > VPR [67041](https:/bdsc.indiana.edu/stocks/67041) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mC]=GAL4-Mef2.R}R1/(TM6B,Tb[1]) C96 > VPR [67042](https:/bdsc.indiana.edu/stocks/67042) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mW.hs]=GawB}bbg[C96]/TM6B,Tb[1] Lpp > VPR [67043](https:/bdsc.indiana.edu/stocks/67043) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO;P{Lpp-GAL4.B}/TM6B,Tb[1] Mhc > VPR [67044](https:/bdsc.indiana.edu/stocks/67044) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO;P{[ry+]MHC-82-Gal4}/TM6B, Tb[1] dpp > VPR [67045](https:/bdsc.indiana.edu/stocks/67045) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; P{w[+mW.hs]=GAL4-dpp.blk1}TM6B,Tb[1] hh > VPR [67046](https:/bdsc.indiana.edu/stocks/67046) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; hh-Gal4 /TM6B,Tb[1] Delta[ts] > VPR in progress w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO;P{w[+mW.hs]=GawB}Dl[05151] ,P{w[+mC]=tubP-GAL80[ts]}[*] /TM6B,Tb[1] tubulin > VPR in progress w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40/CyO; tub-Gal4 /TM6B,Tb[1] tubulin > VPR [67048](https:/bdsc.indiana.edu/stocks/67048) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40; tub-Gal4 / SM5;TM6B Tb[1] en > VPR [67049](https:/bdsc.indiana.edu/stocks/67049) w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/ CyO;UASP{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] spalt > VPR [67050](https:/bdsc.indiana.edu/stocks/67050) w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] blistered > VPR [67051](https:/bdsc.indiana.edu/stocks/67051) w[*]; P{w[+mC]=bs-GAL4.Term}G1/ CyO;UAS:P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] nos > VPR [67052](https:/bdsc.indiana.edu/stocks/67052) w[*]; P{w[+mC]=GAL4-nos.NGT}40 / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] twi > VPR [67053](https:/bdsc.indiana.edu/stocks/67053) w[*]; P{w[+mC]=GAL4-twi.2xPE}1 / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] esg > VPR + GFP [67054](https:/bdsc.indiana.edu/stocks/67054) w[*];esg-Gal4,P{ UAS-GFP} / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] nubbin > VPR [67055](https:/bdsc.indiana.edu/stocks/67055) w[*]; P{w[+mW.hs]=GawB}nubbin-AC-62 / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] 3.1Lsp2 > VPR in progress w[*]; P{w{[+mC]=Gal4-Lsp2-3.1} / CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] Myo1A > VPR [67057](https:/bdsc.indiana.edu/stocks/67057) w[*]; P{GawB}Myo31DFNP0001/ CyO;P{UAS-3XFLAG-dCas9-VPR}attp2/TM6B, Tb[1] ### Temporal control of gene activation - cross to TRiP-OE sgRNA stocks (VPR colletion) Nickname Availability Genotype elav[ts] > VPR [67058](https:/bdsc.indiana.edu/stocks/67058) P{w[+mW.hs]=GawB}elav[C155]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO Lpp[ts] > VPR [67061](https:/bdsc.indiana.edu/stocks/67061) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO;P{Lpp-GAL4.B}/TM6B,Tb[1] Mhc[ts] > VPR [67062](https:/bdsc.indiana.edu/stocks/67062) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO; P{[ry+]MHC-82-Gal4}/TM6B, Tb[1] dMef2[ts] > VPR [67063](https:/bdsc.indiana.edu/stocks/67063) w[*]; P{UAS-3XFLAG-dCas9-VPR}attp40, P{w[+mC]=tubP-GAL80[ts]}10]/CyO; P{w[+mC]=GAL4-Mef2.R}R1/(TM6B,Tb[1]) Myo1A[ts] > VPR [67067](https:/bdsc.indiana.edu/stocks/67067) w[*]; P{GawB}Myo31DFNP0001/CyO; P{UAS-3XFLAG-dCas9-VPR}attp2, P{w[+mC]=tubP-GAL80[ts]}2/TM6B,Tb[1] 3.1Lsp2 > VPR in progress w[*]; P{w{[+mC]=Gal4-Lsp2-3.1}/CyO; P{UAS-3XFLAG-dCas9-VPR}attp2, P{w[+mC]=tubP-GAL80[ts]}2/TM6B,Tb[1] bs[ts]> VPR [67071](https:/bdsc.indiana.edu/stocks/67071) w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2 en[ts]> VPR [67069](https:/bdsc.indiana.edu/stocks/67069) w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2 spalt[ts] > VPR [67070](https:/bdsc.indiana.edu/stocks/67070) w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2 dpp[ts]> VPR [67066](https:/bdsc.indiana.edu/stocks/67066) w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GAL4-dpp.blk1}40C.6/TM6B, Tb[1] C96[ts] > VPR [67064](https:/bdsc.indiana.edu/stocks/67064) w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mW.hs]=GawB}bbg[C96] MS1096[ts]>VPR [67059](https:/bdsc.indiana.edu/stocks/67059) w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10 Dl[ts]> VPR [67047](https:/bdsc.indiana.edu/stocks/67047) w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40/CyO; P{w[+mW.hs]=GawB}Dl[05151-G], P{w[+mC]=tubP-GAL80[ts]}2/TM6B, Tb[1] pnr[ts]>VPR [67060](https:/bdsc.indiana.edu/stocks/67060) w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/TM6B, Tb[1] tubulin > VPR [67065](https:/bdsc.indiana.edu/stocks/67065) w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[1] esg[ts]> VPR [67072](https:/bdsc.indiana.edu/stocks/67072) w[*]; P{w[+mW.hs]=GawB}NP5130 P{w[+*]=UAS-GFP.U}2; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP2, P{w[+mC]=tubP-GAL80[ts]}2 ## Gal4+Cas9 (for tissue specific gene KO) ![Gal4+Cas9 stocks](/sites/g/files/omnuum5366/files/fly/files/screen_shot_2018-07-20_at_11.01.32_am.png) **Cross Gal4+Cas9 stocks to sgRNA stocks from the [TRiP-KO collection](/trip-knockout) (sgRNAs cloned into pCFD3 or pCFD4) to cut target gene.** Somatic tissue-specific GAL4/UAS expression of Cas9 Sort ### Gene cutting/knockout in somatic cells to make mutant tissue - cross to TRiP-KO sgRNA stocks Nickname Availability Genotype elav > Cas9 [67073](https://bdsc.indiana.edu/stocks/67073) P{w[+mW.hs]=GawB}elav[C155]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO ms1096 > Cas9 [67074](https://bdsc.indiana.edu/stocks/67074) w[1118] P{w[+mW.hs]=GawB}Bx[MS1096]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO dMef2 > Cas9 [67075](https://bdsc.indiana.edu/stocks/67075) w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mC]=GAL4-Mef2.R}R1/(TM6B,Tb[1]) C96 > Cas9 [67076](https://bdsc.indiana.edu/stocks/67076) w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mW.hs]=GawB}bbg[C96]/TM6B,Tb[1] Pannier > Cas9 [67077](https://bdsc.indiana.edu/stocks/67077) w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{w[+mW.hs]=GawB}pnr[MD237]/(TM6B,Tb[1]) Lpp > Cas9 in progress w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{Lpp-GAL4.B}/TM6B,Tb[1] Mhc > Cas9 [67079](https://bdsc.indiana.edu/stocks/67079) w[*]; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP40/CyO; P{[ry+]MHC-82-Gal4}/TM6B, Tb[1] actin > Cas9 in progress w[*]; P{w[+mC]=Act5C-GAL4}25FO1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu en > Cas9 in progress w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-2xEGFP}AH2/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu spalt > Cas9 [67081](https://bdsc.indiana.edu/stocks/67081) w[*]; P{w[+mW.hs]=GawB}salm[LP39]/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu blistered > Cas9 [67082](https://bdsc.indiana.edu/stocks/67082) w[*]; P{w[+mC]=bs-GAL4.Term}G1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu twi > Cas9 [67084](https://bdsc.indiana.edu/stocks/67084) w[*]; P{w[+mC]=GAL4-twi.2xPE}1/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu esg > Cas9 + GFP [67085](https://bdsc.indiana.edu/stocks/67085) w[*];esg-Gal4,P{ UAS-GFP}/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B nubbin > Cas9 [67086](https://bdsc.indiana.edu/stocks/67086) w[*]; P{w[+mW.hs]=GawB}nubbin-AC-62/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu 3.1Lsp2 > Cas9 [67087](https://bdsc.indiana.edu/stocks/67087) w[*]; P{w{[+mC]=Gal4-Lsp2-3.1}/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B Myo1A > Cas9 [67088](https://bdsc.indiana.edu/stocks/67088) w[*]; P{GawB}Myo31DFNP0001/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu ## nanos-Cas9 (for gene KO in germline) ![Germline+Cas9](/sites/g/files/omnuum5366/files/fly/files/screen_shot_2018-07-20_at_11.21.42_am.png) **Cross nanos+Cas9 stocks to sgRNA stocks from the [TRiP-KO collection](/trip-knockout) (sgRNAs cloned into pCFD3 or pCFD4) to cut target gene.** germline-specific GAL4/UAS expression of Cas9 to make mutant animals Sort ### Gene cutting/knockout in somatic cells to make mutant tissue - cross to TRiP-KO sgRNA stocks Nickname Availability Genotype nos-Cas9 in progress y[1] sc[*] v[1]; P{Nos-Cas9}Attp40 nos-Cas9 in progress y[1] sc[*] v[1]; P{Nos-Cas9}Attp2 nos > Cas9 [67083](https://bdsc.indiana.edu/stocks/67083) w[*]; P{w[+mC]=GAL4-nos.NGT}40/CyO; P{y[+t7.7] w[+mC]=UAS-Cas9.P2}attP2/TM6B,Tb,Hu