#  Online tools 

 



 ##  

  expand\_more  

 
  

 

Sort***Used our tools in a study? Please cite*** [Hu et al. (2025) NAR Database Issue](https://pubmed.ncbi.nlm.nih.gov/39435987/) ***and individual tool publications.***

**Multi-Species**

 

 

 

 

 ![Square logo for DIOPT online resource](/sites/g/files/omnuum5366/files/fly/files/sq-diopt.jpg)

 

  
[DIOPT ortholog search](https://www.flyrnai.org/cgi-bin/DRSC_orthologs.pl)  
10 species, 18 algorithms  
\[[Demo Video](https://www.youtube.com/watch?v=-RX56ZGX7gY)\]



[ ![Square logo for G2F online resource](/sites/g/files/omnuum5366/files/fly/files/sq-g2f_01.jpg)

 ](http://www.gene2function.org/search/)  
[Gene2Function](http://www.gene2function.org/search/)  
orthologs & gene info summaries (orthologs, GO, & more)  
\[[Demo Video](https://youtu.be/ZUceSmUnjdc)\]



[ ![Square logo for BioLitMine online resource](/sites/g/files/omnuum5366/files/fly/files/sq-biolitmine.jpg)

 ](https://www.flyrnai.org/tools/biolitmine/web/)  
[BioLitMine](https://www.flyrnai.org/tools/biolitmine/web/)  
literature mining tool (genes, pathways, people, MeSH terms)  
\[[Demo Video](https://youtu.be/IzVaOmz517k)\]



[ ![Square logo for MIST](/sites/g/files/omnuum5366/files/fly/files/sq-mist.jpg)

 ](https://fgrtools.hms.harvard.edu/MIST/)  
[MIST](https://fgrtools.hms.harvard.edu/MIST/)  
protein-protein & genetic interactions  
(multi-source)  
\[[Demo Video](https://youtu.be/FoSWELNxVw0)\]



   ![Pangea logo (PAthway, Network and Gene-set Enrichment Analysis)](/sites/g/files/omnuum5366/files/styles/hwp_1_1__100x100_scale/public/fly/files/pangea-logov2.png?itok=o_tYm_fT) 

 

[PANGEA](https://www.flyrnai.org/tools/pangea/)

(**PA**thway, **N**etwork and **G**ene-set **E**nrichment **A**nalysis)



**Fly CRISPR**

 

 

 

**Fly PTMs**

 ![Square icon for fly sgRNA database and LIMS](/sites/g/files/omnuum5366/files/fly/files/sq-sgrna-db-lims-150.jpg)

 

  
[TRiP sgRNA LIMS](https://www.flyrnai.org/tools/grna_tracker/web/)  
nominate or track TRiP-KO & -OE fly stock production



[ ![Square icon for Find CRISPRs online resource](/sites/g/files/omnuum5366/files/fly/files/findcrisprs-close-150.jpg)

 ](https://www.flyrnai.org/crispr/)  
[Find CRISPRs](https://www.flyrnai.org/crispr/)  
fly sgRNA designs with genome view  
(2017 version)



 ![CRISPR3](/sites/g/files/omnuum5366/files/fly/files/crsipr3_thumb.jpg)

 

[Find CRISPRs 3](https://www.flyrnai.org/crispr3/)  
fly sgRNA designs with genome view  
(2019 version)  
\[[Demo Video](https://youtu.be/SNl6k-BhCmY)\]



 ![SNP CRISPR logo icon](/sites/g/files/omnuum5366/files/fly/files/snp-crispr_01.png)

 

[SNP CRISPR](https://www.flyrnai.org/tools/snp_crispr/web/)  
design allele-specific sgRNA for major model organisms



[ ![Square logo for iProteinDB online resource](/sites/g/files/omnuum5366/files/fly/files/sq-iproteindb.jpg)

 ](https://www.flyrnai.org/tools/iproteindb/web/)[iProteinDB](https://www.flyrnai.org/tools/iproteindb/web/)  
post-translational modifications  
\[[Demo Video](https://youtu.be/fuFmaF5or3M)\]



**Fly RNAi**

 

 

 

 

[ ![Square logo for UP-TORR online resource](/sites/g/files/omnuum5366/files/fly/files/sq-uptorr.jpg)

 ](https://www.flyrnai.org/up-torr/)  
[UP-TORR](https://www.flyrnai.org/up-torr/)  
cell and in vivo RNAi reagent search



[ ![Square logo for SnapDragon online resource](/sites/g/files/omnuum5366/files/fly/files/sq-snapdragon.jpg)

 ](https://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl)  
[SnapDragon](https://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl)  
design dsRNAs for  
fly cell RNAi  
\[[Demo Video](https://youtu.be/anXEZLATNIA)\]



[ ![Square logo for RSVP Plus](/sites/g/files/omnuum5366/files/fly/files/sq-rsvpplus.jpg)

 ](https://www.flyrnai.org/cgi-bin/RSVP_search.pl)[RSVP Plus](https://www.flyrnai.org/cgi-bin/RSVP_search.pl)  
in vivo CRISPR & RNAi phenotype data



[ ![Square logo for DRSC Screen Summary online data view](/sites/g/files/omnuum5366/files/fly/files/sq-screensummary.jpg)

 ](https://www.flyrnai.org/screensummary)  
[Screen Summary](https://www.flyrnai.org/screensummary)  
browse DRSC cell RNAi screen data sets



[GeneLookup](https://www.flyrnai.org/cgi-bin/DRSC_gene_lookup.pl)  
(search DRSC & TRiP reagents by gene)  
  
[TRiP Batch Query](https://www.flyrnai.org/cgi-bin/TRiP_batch_query.pl)  
(make a TRiP fly stock list from a gene list)

**RNASeq/ scRNASeq**  
**Resources**

 

**More fly resources**

 

 

[ ![Square logo for DGET online resource](/sites/g/files/omnuum5366/files/fly/files/sq-dget.jpg)

 ](https://www.flyrnai.org/tools/dget/web/)[DGET](https://www.flyrnai.org/tools/dget/web/)  
mine bulk RNAseq data for fly



 ![Screenshot and logo for the DRscDB single-cell resource](/sites/g/files/omnuum5366/files/fly/files/drscdb.jpg)

 

[DRscDB single-cell portal](https://www.flyrnai.org/tools/single_cell/web/)



[ ![Square logo for GLAD online resource](/sites/g/files/omnuum5366/files/fly/files/sq-glad.jpg)

 ](https://www.flyrnai.org/tools/glad/web/)[GLAD](https://www.flyrnai.org/tools/glad/web/)  
view grouped gene lists for fly



 ![Square logo for FlyPrimerBank](/sites/g/files/omnuum5366/files/fly/files/sq-flyprimerbank.jpg)

 

[FlyPrimerBank](https://www.flyrnai.org/cgi-bin/DRSC_primerbank.pl)  
find qPCR primers  
for fly studies  
\[[Demo Video\]](https://www.youtube.com/watch?v=TYq2aHTFSrU&t=32s)



[Paralogs Explorer](https://www.flyrnai.org/tools/paralogs/web/)  
find paralogs & info

More fly resource and utility tools

[Cell Line Expression](/cellexpressionlevels)  
  
[List of Utility Tools](/utility-tools)



**Network & Pathway**

 

 

 

**Transcription Factor-Target Gene Analyses**

[COMPLEAT](https://www.flyrnai.org/compleat/)  
protein complex enrichment analysis  
(fly, human, yeast)  
[[Demo Video](https://youtu.be/aR3t7qnki1s)]

 ![fly perdictome logo](/sites/g/files/omnuum5366/files/fly/files/screenshot_from_2024-04-11_14-40-18.png)

 

  
[FlyPredictome Data Portal](https://www.flyrnai.org/tools/fly_predictome/web/)



[ ![FlyPhone online resource logo icon](/sites/g/files/omnuum5366/files/fly/files/flyphone_logo_01.png)

 ](https://www.flyrnai.org/tools/fly_phone/web/)[Flyphone](https://www.flyrnai.org/tools/fly_phone/web/)  
predict cell-cell communication from scRNAseq data



[ ![Screenshot from the PathOn online resource home page](/sites/g/files/omnuum5366/files/fly/files/screen_shot_2020-10-08_at_9.28.52_am.png)

 ](https://www.flyrnai.org/tools/pathon/web/)  
[PathOn](https://www.flyrnai.org/tools/pathon/web/)  
find pathway status in your RNAseq data



Sort ![Logo for the TF2TG online resource representing transcriptional control of a target gene by transcription factors in cartoon form](/sites/g/files/omnuum5366/files/2026-05/TF2TG_logo3.png)

 

  
[TF2TG](https://www.flyrnai.org/tools/tf2tg/web/)













**Data Sets**

 

 

 

 

[List of Data Sets](/publications/publication-type/dataset-or-data-portal)  
[(DRSC & Perrimon)](/publications/publication-type/dataset-or-data-portal)  
[with links](/publications/publication-type/dataset-or-data-portal)

[Single-cell RNAseq data sets for specific tissues](https://www.flyrnai.org/scRNA/)

[InsulinNet fly PPI, RNAi, PTM data](https://fgrtools.hms.harvard.edu/InsulinNetwork/)  
[Vinayagam et al.](https://fgrtools.hms.harvard.edu/InsulinNetwork/)

[MitoMax fly mitochondrial proteomics data set](http://genepath.med.harvard.edu/perrimon/MitoMax.html)  
[Chen et al.](http://genepath.med.harvard.edu/perrimon/MitoMax.html)

[PhosphoSite fly kinase network data set](https://www.flyrnai.org/PhosphoSite.html)  
[Sopko et al.](https://www.flyrnai.org/PhosphoSite.html)

[Pooled CRISPR fly cell screen raw data sets](https://sharehost.hms.harvard.edu/genetics/?perrimon/CRISPR_fitness_screen_reads)  
[Viswanatha et al.](https://sharehost.hms.harvard.edu/genetics/?perrimon/CRISPR_fitness_screen_reads)

[Nucleolar fly cell RNAi image-based screen data set](https://www.flyrnai.org/tools/drsc_images/web/)  
[Neumuller et al.](https://www.flyrnai.org/tools/drsc_images/web/)

[DRSC RNA Binding RNAi library fly cell screen data set](https://www.flyrnai.org/DRSC-RBP_data.php)  
[Mohr et al.](https://www.flyrnai.org/DRSC-RBP_data.php)

[Table of all public DRSC cell-based RNAi screen data sets at Screen Summary](https://www.flyrnai.org/screensummary)

[FlyBi Drosophila Y2H binary interaction data DRSC/CCSB/BDGP](http://flybi.hms.harvard.edu/)

**Mosquito resources**

 

**Other**

 

 

[Ortholog Mapping](https://www.flyrnai.org/tools/fly2mosquito/web/)  
[(D. mel <-> mosquitos)](https://www.flyrnai.org/tools/fly2mosquito/web/)  
[[Demo Video](https://youtu.be/2qze_VMWNqo)]

[CRISPR sgRNA designs](https://www.flyrnai.org/tools/fly2mosquito/web/)  
[(mosquitos)](https://www.flyrnai.org/tools/fly2mosquito/web/)  
[[Demo Video](https://youtu.be/2qze_VMWNqo)]

[MARRVEL](http://marrvel.org/)  
Connect human gene variants to ortholog info (multi-source)

[DIOPT-DIST](https://www.flyrnai.org/diopt-dist)  
Connect disease genes to ortholog info or vice versa (OMIM & GWAS)



[InsulinNet](https://fgrtools.hms.harvard.edu/InsulinNetwork/)  
fly insulin network data (PPI, RNAi, PTM +/- insulin)  
[[Demo Video](https://youtu.be/zC83eFCH7IU)]

  
[CRIMIC](https://www.flyrnai.org/tools/crimic/web/)  
nominate for GDP gene trap fly stocks -->

Signed PPI/

DirectedPPI

No Longer available.







CONTACT US

[report a bug or make a suggestion](https://hms.az1.qualtrics.com/jfe/form/SV_cApK7Mvnw54HeyV)

***NOTE: Due to a planned institutional IT hardware move, DRSC online tools might not be reachable or functional during part or all of the period January 3 - January 10, 2025. Thank you for your understanding.***



 

##  Citations help us keep going 

Be sure to cite our online resources if they contribute to a published study. You can cite our latest *Nucleic Acids Research* Database Issue paper and/or the publication(s) corresponding to the specific online resource(s) that you used. Please see our [publications page](/publications "Publications").