#  sgRNA vectors 

 



 ##  

  expand\_more  

 
  

 

## Features of all vectors used for in vivo sgRNA expression in TRIP-CRISPR stocks:

- vermilion as a selectable marker
- attB sequence to allow for phiC31 targeted integration at genomic attP landing sites
- ubiquitous sgRNA expression driven by one or more RNA polymerase III-dependent promoters from U6 snRNA genes

## flySAM2.0 (Jia et al., 2018)

- ubiquitously expresses one sgRNA containing MS2 loops from the U6:2 promoter
- UAS control of dCas9-VP64-T2A-MCP
- vector for the TRiP-CRISPR Overexpression (TriP-OE) flySAM2.0 Collection
- [**PDF**](/file_url/640)

## U6B-sgRNA2.0 (Jia et al., 2018)

- ubiquitously expresses one sgRNA containing MS2 loops from the U6:2 promoter
- vector for the TRiP-CRISPR Overexpression (TriP-OE) flySAM1.0 Collection
- [**PDF**](/file_url/641)

## pCFD3 (Port, Chen, Lee, & Bullock, 2014)

- ubiquitously expresses one sgRNA from the U6:3 promoter
- vector for TRiP-CRISPR Knockout (TriP-KO) Collection
- For more information visit <http://www.crisprflydesign.org/plasmids/> and [addgene](https://www.addgene.org/49410/)
- [**PDF**](/file_url/549)

## pCFD4 (Port, Chen, Lee, &amp; Bullock, 2014)

- ubiquitously expresses two sgRNAs from the U6:1 and U6:3 promoters
- primary vector of the TRiP-CRISPR Overexpression (TRiP-OE) VPR Collection
- For more information visit <http://www.crisprflydesign.org/plasmids/> and [addgene](https://www.addgene.org/49411/)
- [**PDF**](/file_url/550)

## pCFD4-MS2

- ubiquitously expresses two sgRNAs containing MS2 loops from the U6:1 and U6:3 promoters
- compatible with the Synergistic Activation Mediator (SAM) system (Konermann et al., 2015)
- [**PDF**](/file_url/551)

## pl100 (Kondo and Ueda, 2013)

- ubiquitously expresses one sgRNA from the U6:1 promoter
- [**PDF**](/file_url/552)



 

##  Relevant publications 

 Yu Jia, Rong-Gang Xu, Xingjie Ren, Ben Ewen-Campen, Rajendhran Rajakumar, Jonathan Zirin, Donghui Yang-Zhou, Ruibao Zhu, Fang Wang, Decai Mao, Ping Peng, Huan-Huan Qiao, Xia Wang, Lu-Ping Liu, Bowen Xu, Jun-Yuan Ji, Qingfei Liu, Jin Sun, Norbert Perrimon, and Jian-Quan Ni. **"Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM"** PNAS. 2018 May 1;115(18):4719-4724. PubMed ID: [29666231](https://www.ncbi.nlm.nih.gov/pubmed/29666231)

 Silvana Konermann, Mark D. Brigham, Alexandro E. Trevino, Julia Joung, Omar O. Abudayyeh, Clea Barcena, Patrick D. Hsu, Naomi Habib, Jonathan S. Gootenberg, Hiroshi Nishimasu, Osamu Nureki &amp; Feng Zhang. 2015. **"Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex"** Nature. 2015 Jan 29;517(7536):583-8.. PubMed ID: [25494202](https://www.ncbi.nlm.nih.gov/pubmed/25494202)

 Fillip Port, Hui-Min Chen, Tzumin Lee, and Simon L. Bullock. 2014. **"Optimixed CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila."** PNAS. 2014 Jul 22;111(29):E2967-76. PubMed ID: [25002478](https://www.ncbi.nlm.nih.gov/pubmed/25002478)

 Shu Kondo, Ryu Ueda. 2013. **"Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in *Drosophila.*"** Genetics. 2013 Nov;195(3):715-21. PubMed ID: [24002648](https://www.ncbi.nlm.nih.gov/pubmed/24002648)