#  Reagents 

 



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Our reagents fall into two main categories, those for *Drosophila* cell culture and those for *in vivo* studies, and include two main technologies, RNAi and CRISPR applications. We also have reagents relevant to the study of miRNAs and for overexpression of open reading frames (ORFs).

Reagents for *in vivo* *Drosophila* studies include

- TRiP RNAi fly stocks, which can be
    - learned about at [our TRiP RNAi fly stocks overview page](/trip-in-vivo-fly-rnai)
    - searched at [UP-TORR](/up-torr) (search for RNAi fly stocks vs. up-to-date gene annotations)
    - searched at [RSVP](/rsvp) (view existing data on knockdown efficiency or phenotypes alongside stock information)
    - requested from the [Bloomington *Drosophila* Stock Center (BDSC)](http://flystocks.bio.indiana.edu/)
    - viewed as curated lists (e.g. kinases) on [our TRiP fly stocks page](/trip-rnai-fly-stocks)
- TRiP-CRISPR fly stocks, which can be
    - viewed at [our CRISPR fly stocks page](/crispr-fly-stocks-and-vectors)
    - searched and nominated at the [DRSC/TRiP sgRNA Stock Tracking System](http:/www.flyrnai.org/tools/grna_tracker/web/)
- molecular reagents for building your own shRNA or overexpression fly stocks based on [the VALIUM series of plasmid vectors](/trip-plasmid-vector-sets) (requests via the [DF/HCC DNA Resource Core PlasmID repository](https://plasmid.med.harvard.edu/PLASMID/Home.xhtml))

Reagents for cell-based studies are directly distributed by our facility and include

- [DRSC 2.0 genome-wide RNAi library](/drsc-20-genome-wide-screening-library) in 384-well plate format
- [DRSC focused sub-libraries (e.g. kinases)](/drsc-focused-sub-libraries) in 384-well plate format
- 'cherry picks' of PCR templates from our collection for in vitro transcription in your lab
- custom 96-well format plates of dsRNAs made from templates in our collection
- for all of the above, orders should be initiated via [our DRSC-FGR PPMS system](/ordering)